TCM Stage Differentiation Treatment of Diabetic Gangrene——An Observation on Microcirculatory Changes

Microcirculatory changes and therapeutic effects in 39 non-insulin dependent diabetes mellitus (NIDDM) cases were evaluated. Under the same basic treatment in both groups, the treatment group (21 cases) was treated with TCM drugs according to its stages and compared with the control group (18 cases, treated with 654-2). The results showed that the microcirculatory cumulative scores were significantly decreased in both groups (P<0.05), but the decrease in the treatment group was more marked (P<0.05). In the treatment group, 15 cases were cured, 3 markedly effective, 2 effective and 1 ineffective; while in the control group, 13 cases were cured, 1 markedly effective and 4 ineffective. Statistically, there is no significant difference between the 2 groups (P>0.05) in therapeutic effects.

Relationship of Adrenocortical Function and TCM Syndrome Typing in Elderly Patients with Severe Sepsis

Objective: To explore the relationship between TCM Syndrome typing and adrenocortical function in elderly patients with severe sepsis, and to see whether TCM Syndrome Differentiation can provide clinical clues in identifying relative adrenal insufficiency (RAI) in patients with severe sepsis. Methods: Sixty-one old patients with severe sepsis were classified into four types according to TCM Syndrome Differentiation: The severe invasion of toxic-heat type (Type SITH, n = 21); the Qi stagnation and blood stasis type (Type QSBS, n = 11); the sudden depletion of Yang-Qi type (Type SDYQ, n = 16); and the exhaustion of Qi-Yin type (Type EOQY, n = 13). The base-line level of plasma cortisol in patients of different types and their response to corticotropin stimulation were compared, which were also compared with those of 12 healthy elderly persons synchronously. Results: The base-line level of plasma cortisol was not significantly different between patients of different Syndrome types ( P>0. 05), but they were all sgnificantly higher than that in the healthy persons (P<0. 05). Compared with Type QSBS and Type EOQY, Type SITH and Type SDYQ showed less cortisol concentration increment after corticotropin stimulation ( P<0. 05). RAI was more prevalent in patients of Type SITH and Type SDYQ than in patients of Type QSBS and Type EOQY (57% vs 25% , P<0. 01). Conclusion: In old patients with severe sepsis, different TCM Syndrome types are associated with different adrenocortical function status. TCM Syndrome differentiation can provide clinical clues in i-dentifying old patients with severe sepsis who have also RAI.

Relationship between Traditional Chinese Medicine Syndrome Type and Coronary Arteriography of Acute Coronary Syndrome

Objective: To explore the relationship of Traditional Chinese Medicine (TCM) Syndrome type and coronary arteriography (CAG) with respect to the number and degree of stenosed branches of coronary artery (CA) and ACC/AHA stage of acute coronary syndrome (ACS), to provide an objective evidence for TCM Syndrome typing on ACS Methods: Ninety patients of ACS with their TCM Syndrome typing and CAG successfully conducted were enrolled in this study. They were classified into 3 Syndrome types, the blood stasis type (type I ), the phlegm stagnant with blood stasis type (type II ),and the endogenous collateral Wind type (type III) The scores of the number and severity of the stenosis branch of CA and ACC/AHA lesion stage in different Syndrome types were calculated respectively and analysed statistically by Ridit analysis. Results: The number of stenosed branches increased gradually with the Syndrome type changing from I -II-III, compared the type III with the other two types(P<0. 01). The severity of stenosis in type I and II were similar, but that of Type III, much aggravated was significantly different from that in the former two (P<0. 01) . The ACC/AHA stage of coronary lesion tended to be more complex as the Syndrome type changed, patients of TCM type I and II had mostly lesion of stage A or Bl , while lesion in majority of patients of type III belonged to stage B2 or C, comparison between the three types showed significant difference (P<0.01). Conclusion: Most ACS patients of TCM Syndrome type III with tri-branch, severe stenosed coronary arteries, belong to the complex ACC/AHA stage of B2 and C.

Effect of Kaiyu Qingwei Granule (开郁清胃颗粒) on Insulin Receptor in Liver and Skeletal Muscular Cell Membrane in Diabetes Mellitus Rats

Objective: To investigate the effect of Kaiyu Qingwei granule (KYQWG, on the insulin binding capacity of liver and skeletal muscular cell membrane and serum insulin-like growth factor-1 (IGF-1) in streptozotocin-induced diabetic rats. Methods: Rats in four experimental groups were investigated: the control group, the model group, the KYQWG group and the Metformin group. The insulin binding rate (IBR) of liver and skeletal muscular cell membrane was detected by receptor-ligand ra-diometric method and changes of serum levels of glucose, insulin and IGF-1 were observed before and after 4 weeks of medication. Results: The KYQWG group had a lower blood glucose level and ffiR of liver and muscular cell membrane, as compared with those in the model group (P<0. 01 or P<0.05), and a higher level of IGF-1 than that in the model group(P<0.01), but had no obvious changes in the serum level of insulin. Conclusion: KYQWG may increase the serum level of IGF-1 in diabetic rats, thus to decrease the insulin resistance at ante-receptor sites and improve the sugar metabolic disturbance in rats with diabetes mellitus.

Anti-inflammatory and profibrinolytic effect of tetramethylpyrazine in acute coronary syndromes

Tetramethylpyrazine (TMP) is a herb used widely in Traditional Chinese Medicine (TCM) as an antianginal drug. The exact mechanism whereby TMP treat ischemic heart disease is still not fully understood. The purpose of this study is to examine the anti-inflammatory effect of TMP in patients with acute coronary syndromes (ACS). Methods Thirty-two patients with acute myocardial infarction or unstable angina were randomly assigned to TMP group or control group. All patients received the same standard treatment. Patients in TMP group received TMP 3mg/kg every 12 hours for 5 days. Plasma concentrations of high-sensitivity Creactive protein (CRP), serum amyloid A (SAA) and plasminogen activator inhibitor-1 (PAI-1) were measured at baseline and after 5 days of therapy. Results Both CRP and SAA concentrations increased significantly in control group (P＜0.05) whilst in TMP group, only SAA had a significant increase (P＜0.05); the absolute increase of CRP, SAA, and PAI-1 were significantly less in TMP group than in control group (P＜0.05). Conclusion TMP has an anti-inflammatory and profibrinolytic effect in patients with ACS. These effects may contribute to the clinical benefits of TMP in ischemic heart disease.

The Mechanism of the combination of radix paeoniae rubra-cortex moutan in treating cerebral hemorrhage based on network pharmacology

Objective:To clarify the material basis of Chinese medicine pair“Radix Paeoniae Rubra-Cortex Moutan”(Chishao-Mudanpi)and explore their mechanism in the treatment of ICH with network pharmacology.Methods:The active ingredients contained in Radix Paeoniae Rubra and Cortex Moutan were searched and selected based on the oral bioavailability prediction and drug-likeness prediction from the TCMSP database.Then the targets of cerebral hemorrhage were collected from GeneCards,OMIM,and DrugBank databases.After obtained the intersections of drugs and disease,the active component target disease interactive network diagram was drawn by Cytoscape software.The obtained key targets were uploaded to the STRING database for analysis and construct a PPI network map.GO function enrichment analysis and KEGG analysis were performed on the key target proteins.Results:Collected the active ingredients of Radix Paeoniae 119,Radix Paeoniae 55,including paeoniflorin,baicalin,β-sitosterol,etc.Related drug target protein 1190,ICH disease-related genes 823,"Radix Paeoniae-Radix Paeoniae"and 72 common targets of ICH,mainly acting on Akt1,IL6,VEGFA,CASP3,EGF,involving 133 related signaling pathways such as AGE-RAGE,TNF,IL-17,HIF1,PI3K-Akt.Conclusion:The combination of"Radix Paeoniae Rubra-Cortex Moutan"in the treatment of ICH has the characteristics of multiple pathways and multiple targets,which provides a reference and basis for further molecular biology verification in the future.

Pseudobulbar Paralysis Treated by Acupuncture——Clinical Observation in 36 Cases

Pseudobulbar paralysis is a kind of common clinical syndromes of cerebral vascular diseases, which is manifested as dyslalia, dysphagia and choking. By several-year clinical observations, 36 cases were treated with satisfactory therapeutic effects as reported in the following. Clinical Data Of 36 in-patients, there were 24 males and 12 females, aged from 44 to 81 years, averaging 64.92 years. Of 36 cases, 24 were at the acute stage and 12 at the recovery stage. All the cases were diagnosed as cerebral vascular diseases by cranial CT scan and MRI, of which, 4 were cerebral infarction, 26 lacunar cerebral infarction, 5 cerebral hemorrhage and 1 mixed type. Of 36 cases, 15 were the first attack of wind-stroke, 15 the second attack, 5 the third attack and 1 the forth attack. There were 26 patients with hypertension among 36 cases, of which, 8 patients suffered from hypertension within 10 years, 6 for more than 10 years, 9 for more than 20 years and 3 for more than 30 years. All the 36 cases were associated with dysphagia and agreeable to Standard on Diagnosis and Evaluation of Therapeutic Effects of Wind-stroke issued by the State Scientific Committee 85-919-01-01, 1995.

ACUPUNCTURE AND GLAUCOMA

It is to recognize glaucoma in view of Chinese medicine and synthesize the treatments and mechanisms of acupuncture on glaucoma in the paper. It is indicated in the results that acupuncture plays the importance in lowering ocular tension, improving ocular circulation and protecting optic nerve. Additionally, acupuncture inhibits probably the cellular apoptosis of optic ganglion and provides definite actions on psychological factors of glaucoma. Acupuncture cannot replace operation and medicinal therapy in lowing ocular tension, but their coordinated and auxiliary effects are quite clear and definite.

Study on the relationship between syndrome characteristics degree and inflammatory factor and negative emotional scale in"double heart disease"patients with Qi stagnation blood stasis and heart gallbladder heat stagnation

Objective:To Discuss the correlation between Hypersensitivity C-reactive Protein(Hs-CRP),Total Cholesterol(TC),Triglyceride(TG),negative emotion scale and TCM syndrome scores in“Double Heart Disease”patients with Qi stagnation blood stasis and heart gallbladder heat stagnation.Method:Fifty-two patients in Western Medicine Diagnosis of Double Heart Disease,in TCM syndrome identified as Qi stagnation blood stasis Heart gallbladder heat stagnation syndrome,detects it Hs-CRP,TC,TG levels by ELISA,use TCM Syndromes Scale to evaluate TCM Syndrome,use the Pittsburgh Sleep Quality Index(PSQI)and Self-rating symptom scale(SCL-90)to assess anxiety and sleep levels,analyze the correlation between TCM syndrome scores and Hs-CRP,TC,TG level,PSQI index,SCL-90 index.Result:There was a significant positive correlation between Hs-CRP,TG level and TCM Syndrome scores(P<0.05);TC level was postively correlated with TCM Syndrome scores,but there was no statistical significance(P>0.05);There was a significant positive correlation between PSQI index,SCL-90 index and TCM Syndrome scores(P<0.05).Conclusion:In“Double Heart Disease”patients with Qi stagnation blood stasis and heart gallbladder heat stagnation,there have characteristic syndrome changes in terms of inflammatory factor level,blood lipid level and negative emotion score;Which the above indexes can reflect the severity of TCM syndromes to a certain extent and provide the basis for the effective intervention treatment of TCM.

Clinical Observation on Treatment of Hyperplasia of Mammary Gland by Lirukang Granule (利乳康颗粒)

Objective： To explore the efficacy and mechanism of Lirukang Granule （利乳康颗粒, LRKG） in treating hyperplasia of mammary gland （HMG）. Methods： One hundred patients with HMG were randomly assigned to two groups, 50 in each group. The patients in the treated group were orally administered with LRKG thrice a day, one package each time, and those in the control group were given orally Rukuaixiao Tablet （乳块消, RKX） thrice a day, 4 tablets each time. The therapeutic course for both groups was 4 months. The clinical efficacy, pain alleviating rate, as well as changes of local sign and symptom scores were observed before and after treatment. The changes of serum estradiol （E2）, progesterone （P）, testosterone （T）, follicle stimulating hormone （FSH）, luteinizing hormone （LH） and prolactin （PRL） in some randomly selected patients （24 patients in the treated group and 24 in the control group） before and after treatment were measured with radioimmunoassay. Results： The total clinical efficacy in the treated group was superior to that in the control group, significant difference was shown between the two groups （P〈 0.01）. The cure-effective rate and total effective rate in the treated group were 70.0%and 88.0% respectively, significantly higher than those in the control group （38.0% and 64.0%） respectively （P〈 0.01）, and the pain alleviating rate in the treated group was also significantly higher in the former than that in the latter （88.0% vs 64.0%, P 〈 0.05）. Moreover, the treated group showed obvious superiority in improving the patients＇ symptom and sign scores （P 〈 0.01）, and abnormalities of gonadal hormone as compared with the respective items in the control group （P〈0.01）. Conclusion： LRKG has good efficacy in the treatment of HMG, and its mechanism may be related to the regulation on endocrine and immune function.

Effect of Acupuncture at Acupoint Neiguan(PC6)on the Myocardial c-fos Gene Expression of Acute Myocardial Ischemia in Rats

目的:探讨电针"内关"改善急性心肌缺血的机制。方法:将96只大鼠随机分为假手术组、心肌缺血模型组、心肌缺血模型加电针组,采用结扎冠状动脉左前降支建立急性心肌缺血实验模型,造模成功后,电针双侧"内关"。用生化方法检测血清心肌酶活性,逆转录聚合酶链反应(RT-PCR)方法检测心肌中 c-fos 基因的表达。结果:心肌缺血模型组血清心肌酶活性和心肌 c-fos mRNA 表达显著高于假手术组(P<0.05),电针后降低,差异有显著性意义(P<0.05)。结论:电针"内关"改善急性心肌缺血的机制与下调心肌组织 c-fos mRNA 的表达有关。

Experience of Prof. ZHANG-Ji in Treatment of Intractable Diseases： A Report of 5 Cases

Prof. ZHANG Ji, male, born in Jinzhou City, Liaoning Province in May 1929, distinguished acupuncture expert in China, professor of Beijing University of TCM,doctorate supervisor, the all-China second-batch distinguished and elderly TCM expert, receives the special government allowance issued by the State Council. So far he has been doing acupuncture teaching,scientific research and clinical practice for more than 40years. He once gave lectures in different countries including Italy, Japan, the United States, and France and got favorable comments from the international colleagues. Up to date, he has published more than 50academic papers and 16 books including Differentiation Analysis of Neijing Disease Syndrome, Collections of Acupuncture Classics,Medical Classics of Varieties of Acupuncturists and Acupuncture & Moxibustion. And he has trained more than 20 students for either master degree or doctorate and obtained several scientific and technology achievements.

Observations on the Curative Effect of Auricular Point Sticking Therapy on Vertebral Artery Type of Cervical Spondylosis

目的:评价耳穴贴压法治疗椎动脉型颈椎病的疗效。方法:采用同期随机单盲对照方法。治疗组42例采用耳穴贴压法,对照组38例采用中成药治疗。15 d为1个疗程,最多不超过2个疗程治疗。结果与结论:治疗组总有效率为97.6%,对照组总有效率为73.7%。两组比较差异有统计学意义,治疗组明显优于对照组。

Clinical Study on Effect of Xianlong Capsule (仙龙胶囊) in Treating Senile Vascular Dementia

Objective: To assess the efficacy of Xianlong Capsule (XLC) in treating senile vascular dementia (VD). Methods: Forty six patients of VD were randomly divided into two groups. The patients in XLC group (n=24) were treated with XLC and the patients in the control group (n=22) were treated with Hydergine. The therapeutic effect on dementia, TCM Syndrome, neurofunction deficit score, activities of daily living (ADL) score, Hasegawa Dementia Score (HDS) and mini mental state examination (MMSE), etc. were examined and compared before and after treatment.Results: XLC could markedly increase MMSE scores and HDS of VD patients. It showed effect in ameliorating the TCM Syndromes and neurofunction deficit score significantly and could markedly improve the daily living activity of patients. The effect of XLC was better than that of Hydergine.Conclusion: XLC has definite effect in treating senile vascular dementia.

Effect of Xiaoke Granule(消渴冲剂) on Blood Sugar and Blood Rheological Property in Experimental Diabetic Animals

Objective:To study the pharmacological effect of Xiaoke Granule (XKG, 消渴冲剂) on blood sugar and blood rheological property in the diabetic animals.Methods: Alloxan induced diabetic mice or rats were grouped randomly. The effects of XKG on blood sugar, appetite, capacity of drinking, glucose tolerance, blood lipid and blood rheological property were observed and compared among groups.Results:XKG showed a trend in reducing the appetite and capacity of drinking, increasing the body weight, and significantly inhibiting the increase of blood sugar coused by ectogenic glucose in mice, and could improve the blood lipid and blood rheological property in rats.Conclusion:XKG is effective in reducing serum total cholesterol, lowering the blood viscosity, improving the blood rheological property of alloxan induced diabetic animals. Therefore, it might effective in treating and preventing the occurrence of diabetes mellitus and the complications of blood stasis.