A distributed non-point source pollution model: calibration and validation in the Yellow River Basin

The applicability of a non-point source pollution model—SWAT(soil and water assessment tools) in a large river basin with high sediment runoff modulus(770 t/km2 in the Yellow River) was examined. The basic database, which includes DEM, soil and landuse map, weather data, and land management data, was established for the study area using GIS. A two-stage “Brute Force' optimization method was used to calibrate the parameters with the observed monthly flow and sediment data from 1992 to 1997. In the process of calibration automated digital filter technique was used to separate direct runoff and base flow. The direct runoff was firstly calibrated, and the base flow, then the total runoff was matched. The sediment yield was calibrated to match well. Keeping input parameters set during the calibration process unchanged, the model was validated with 1998—1999's observed monthly flow and sediment. The evaluation coefficients for simulated and observed flow and sediment showed that SWAT was successfully applied in the study area: relative error was within 20%, coefficient of determination and Nash-Suttcliffe simulation efficiency were all equal to or above 0.70 during calibration and validation period.

Effect of Bcl-2 and caspase-3 on calcium distribution in apoptosis of HL-60 cells

Apoptosis manifests in two major execution programs downstream of the death signal: the caspase pathway and organelle dysfunction. An important antiapoptosis factor, Bcl-2 protein, contributes in caspase pathway of apoptosis. Calcium, an important intracellular signal element in cells, is also observed to have changes during apoptosis, which maybe affected by Bcl-2 protein. We have previously reported that in Harringtonine (HT) induced apoptosis of HL-60 cells, there’s a change of intracellular calcium distribution, moving from cytoplast especially Golgi’s apparatus to nucleus and accumulating there with the highest concentration. We report here that caspase-3 becomes activated in HT-induced apoptosis of HL-60 cells, which can be inhibited by overexpression of Bcl-2 protein. No sign of apoptosis or intracellular calcium movement from Golgi’s apparatus to nucleus in HL-60 cells overexpressing Bcl-2 or treated with Ac-DEVD-CHO, a specific inhibitor of caspase-3. The results indicate that activated caspase-3 can promote the movement of intracellular calcium from Golgi’s apparatus to nucleus, and the process is inhibited by Ac-DEVD-CHO (inhibitor of caspase-3), and that Bcl-2 can inhibit the movement and accumulation of intracellular calcium in nucleus through its inhibition on caspase3. Calcium relocalization in apoptosis seems to be irreversible, which is different from the intracellular calcium changes caused by growth factor.

Projection pursuit cluster model and its application in water quality assessment

One of the difficulties frequently encountered in water quality assessment is that there are many factors and they cannot be assessed according to one factor, all the effect factors associated with water quality must be used. In order to overcome this issues the projection pursuit principle is introduced into water quality assessment, and projection pursuit cluster(PPC) model is developed in this study. The PPC model makes the transition from high dimension to one-dimension. In other words, based on the PPC model, multifactor problem can be converted to one factor problem. The application of PPC model can be divided into four parts: (1) to estimate projection index function Q(); (2) to find the right projection direction ; (3) to calculate projection characteristic value of the i th sample z-i, and (4) to draw comprehensive analysis on the basis of z-i. On the other hand, the empirical formula of cutoff radius R is developed, which is benefit for the model to be used in practice. Finally, a case study of water quality assessment is proposed in this paper. The results showed that the PPC model is reasonable, and it is more objective and less subjective in water quality assessment. It is a new method for multivariate problem comprehensive analysis.

Ecological and environmental water demand of the lakes in the Haihe-Luanhe Basin of North China

The purpose of this paper is to present a brief concept of the ecological and environmental water demand of lake. The present situation and affecting factors of lake ecological system in the Haihe\|Luanhe Basin of North China was analyzed. The calculating method of the ecological and environmental water demand of the lake basis on the water body and the calculating method of the ecological and environmental water demand of the lake basis on the aquatic ecosystem, wetland and vegetation were compared and discussed. As the examples of Baiyangdian Lake and Beidagang Lake in Haihe\|Luanhe river basin, the ecological and environmental water demand of the two lakes was calculated to be 27×10\+8m\+3. It is 6.75 times to the water demand according to the calculating method of the ecological and environmental water demand of the lake basis on the water body. The research result indicated: (1) The calculating methods of the ecological and environmental water demand of the lake basis on the aquatic ecosystem should be better than only basis on the water body of lake. (2) The data, such as area of the vegetation kind around and in the lake, the vegetation coefficient, the evaporating amount of the vegetation and the vegetation water demand itself around and in the lake are lack and urgent need. Some suggestions for controlling and regulating the water resource of the lake in North China were proposed.

Mitotic cell death in BEL-7402 cells induced by enediyne antibiotic lidamycin is associated with centrosome overduplication

AIM: Mitotic cell death has been focused on in tumor therapy.However, the precise mechanisms underlying it remain unclear. We have reported previously that enediyne antibiotic lidamycin induces mitotic cell death at low concentrations in human epithelial tumor cells. The aim of this study was to investigate the possible link between centrosome dynamics and lidamycin-induced mitotic cell death in human hepatoma BEL-7402 cells.METHODS: Growth curve was established by Ml-l assay.Cell multinucleation was detected by staining with Hoechst 33342. Flow cytometry was used to analyze cell cycle.Aberrant centrosomes were detected by indirect immunofluorescence. Western blot and senescenceassociated β-galactosidase (SA-β-gal) staining were used to analyze protein expression and senescence-like phenotype,respectively.RESULTS: Exposure of BEL-7402 cells to a low concentration of lidamycin resulted in an increase in cells containing multiple centrosomes in association with the appearance of mitotic cell death and activation of SA-β-gal in some cells, accompanied by the changes of protein expression for the regulation of proliferation and apoptosis. The mitochondrial signaling pathway, one of the major apoptotic pathways, was not activated during mitotic cell death. The aberrant centrosomes contributed to the multipolar mitotic spindles formation, which might lead to an unbalanced division of chromosomes and mitotic cell death characterized by the manifestation of multi- or micronucleated giant cells.Cell cycle analysis revealed that the lidamycin treatment provoked the retardation at G2/M phase, which might be involved in the centrosome overduplication.CONCLUSION: Mitotic cell death and senescence can be induced by treatment of BEL-7402 cells with a low concentration of lidamycin. Centrosome dysregulation may play a critical role in mitotic failure and ultimate cell death following exposure to intermediate dose of lidamycin.

A review of soil erodibility in water and wind erosion research

Soil erodibility is an important index to evaluate the soil sensitivity to erosion. The research on soil erodibility is a crucial tache in understanding the mechanism of soil erosion. Soil erodibility can be evaluated by measuring soil physiochemical properties, scouring experiment, simulated rainfall experiment, plot experiment and wind tunnel experiment. We can use soil erosion model and nomogram to calculate soil erodibility. Many soil erodibility indices and formulae have been put forward. Soil erodibility is a complex concept, it is influenced by many factors, such as soil properties and human activities. Several obstacles restrict the research of soil erodibility. Firstly, the research on soil erodibility is mainly focused on farmland; Secondly, soil erodibility in different areas cannot be compared sufficiently; and thirdly, the research on soil erodibility in water-wind erosion is very scarce. In the prospective research, we should improve method to measure and calculate soil erodibility, strengthen the research on the mechanism of soil erodibility, and conduct research on soil erodibility by both water and wind agents.

Risk forecasting and evaluating model of Environmental pollution accident

Environmental risk(ER) factors come from ER source and they are controlled by the primary control mechanism(PCM) of environmental risk, due to the self failures or the effects of external environment risk trigger mechanism, the PCM could not work regularly any more, then, the ER factors will release environmental space, and an ER field is formed up. The forming of ER field does not mean that any environmental pollution accident(EPA) will break out; only the ER receptors are exposed in the ER field and damaged seriously, the potential ER really turns into an actual EPA. Researching on the general laws of evolving from environmental risk to EPA, this paper bring forwards a relevant concept model of risk forecasting and evaluating of EPA. This model provides some scientific methods for risk evaluation, prevention and emergency response of EPA. This model not only enriches and develops the theory system of environment safety and emergency response, but also acts as an instruction for public safety, enterprise's safety management and emergency response of the accident.

Influence of Arctic Oscillation on winter climate over China

In this study the relationship between the Arctic Oscillation (AO) and climate in China in boreal winter are investigated. Correlation analysis for the last 41 years shows that the winter temperature and precipitation in China change in phase with AO. High positive correlation (>0.4) between temperature and AO appears in the northern China. High correlation coefficients between precipitation and AO cover the southern China (close to the South China Sea) and the central China (between 30 o -40 o N and east of ~100 o E), with the values varying between +0.3 and +0.4. It is found that during the past several decades the precipitation was strongly affected by AO, but for the temperature the Siberian High plays a more important role. At the interdecadal time scale the AO has significant influence on both temperature and precipitation. Multivariate regression analysis demonstrates that AO and the Siberian High related variance in temperature and precipitation is 35% and 11% respectively. For precipitation, however the portion is rather low, implying that some other factors may be responsible for the changes in precipitation, in addition to AO and the Siberian High.

Extreme climate events over northern China during the last 50 years

Climate extremes for agriculture-pasture transitional zone, northern China, are analyzed on the basis of daily mean temperature and precipitation observations for 31 stations in the period 1956-2001. Analysis season for precipitation is May-September, i.e., the rainy season. For temperature is the hottest three months, i.e., June through August. Heavy rain events, defined as those with daily precipitation equal to or larger than 50 mm, show no significant secular trend. A jump-like change, however, is found occurring in about 1980. For the period 1980-1993, the frequency of heavy rain events is significantly lower than the previous periods. Simultaneously, the occurring time of heavy rains expanded, commencing about one month early and ending one month later. Long dry spells are defined as those with longer than 10 days without rainfall. The frequency of long dry spells displays a significant (at the 99% confidence level) trend at the value of +8.3% /10a. That may be one of the major causes of the frequent droughts emerging over northern China during the last decades. Extremely hot and low temperature events are defined as the uppermost 10% daily temperatures and the lowest 10% daily temperatures, respectively. There is a weak and non-significant upward trend in frequency of extremely high temperatures from the 1950s to the mid-1990s. But the number of hot events increases as much as twice since 1997. That coincides well with the sudden rise in mean summer temperature for the same period. Contrary to that, the frequency of low temperature events have been decreasing steadily since the 1950s, with a significant linear trend of-15%/10a.

A Preliminary Analysis of Non-small Cell Lung Cancer Biomarkers in Serum

To identify potential serum biomarkers that could be used to discriminate lung cancers from normal. Methods Proteomic spectra of twenty-eight serum samples from patients with non-small cell lung cancer and twelve from normal individuals were generated by SELDI (Surfaced Enhanced Laser Desorption/Ionization) Mass Spectrometry. Anion-exchange columns were used to fractionate the sera into 6 designated pH groups. Two different types of protein chip arrays, IMAC-Cu and WCX2, were employed. Samples were examined in PBSII Protein Chip Reader (Ciphergen Biosystem Inc) and the discriminatory profiling between cancer and normal samples was analyzed with Biomarker Pattern software. Results Five distinct potential lung cancer biomarkers with higher sensitivity and specificity were found, with four common biomarkers in both IMAC-Cu and WCX2 chip; the remaining biomarker occurred only in WCX2 chip. Two biomarkers were up-regulated while three biomarkers were down-regulated in the serum samples from patients with non-small cell lung cancer. The sensitivities provided by the individual biomarkers were 75%-96.43% and specificities were 75%-100%. Conclusions The preliminary results suggest that serum is a capable resource for detecting specific non-small cell lung cancer biomarkers. SELDI mass spectrometry is a useful tool for the detection and identification of new potential biomarker of non-small cell lung cancer in serum.

Shedding of TNFR1 in regenerative liver can be induced with TNF α and PMA

AIM: Liver regeneration is associated with apoptosis of hepatocytes, which is mediated via tumor necrosis factor receptor 1(TNFR1). The shedding of TNFR1 in liver regeneration and its mechanism to regulate this shedding were investigated.METHODS: The shedding of TNFR1 in liver regeneration and changes of TNF-α, PMA and plasma membrane purified from hepatocytes on this shedding process were measured with Western blot. Then, the relationship between TNFR1 shedding and apoptosis of hepatocytes induced by TNFα was studied by detecting apoptotic index.RESULTS: The shedding of TNFR1 began at 4 hours and terminated before 2 months after partial hepatectomy. In culture system, serum from rats at 36 h after partial hepatectomy could also promote this shedding process. With the stimulation of TNF α, PMA or purified plasma membrane from hepatocytes at 36 h after partial hepatectomy or from hepatocytes treated with TNF α for 2 h, membranous TNFR1 was also shed. With the stimulation of both TNF α and plasma membrane from hepatocytes affected with TNF α for 2 hor from hepatocytes at 36 h after partial hepatectomy, apoptotic index of hepatocytes decreased from 21% to 7.52 % and 8.45 %, respectively. PMA could also reduce apoptotic index to 13.67 %. This descent occurred in hepatocytes cultured in serum from rats at 36 h after partial hepatectomy too,but not in serum from rats at 2 months after partial hepatectomy and sham-operated rats.CONCLUSION: Shedding of TNFR1 may help reduce apoptosis of hepatocytes induced by TNFα. Membraneanchored metalloprotases could play a role in shedding membranous TNFR1. At the same time, PKC may take part in regulation of this shedding process.

The Role of Relationships with Teachers in Adolescent Development among a National Sample of Chinese Urban Adolescents

A nationally representative sample of 1640 Chinese urban adolescents (mean age=14.5 years) and their parents and teachers were surveyed to examine the associations between relationships with teachers and parents and adolescents' developmental outcomes such as academic performance, psychological well-being, altruistic behaviors, and misconduct. Results showed that the quality of adolescents' relationships with teachers was highly correlated with adolescent outcomes, in most cases more so than for relationships with parents. Furthermore, such associations were the same across both genders and three grade levels. These findings suggest that teachers may play an especially significant role in Chinese adolescent development. This study adds to the small but growing literature on the importance of teachers as well as other non-parental adults in adolescent psychosocial development.

BOUNDEDNESS OF COMMUTATORS RELATED TO MARCINKIEWICZ INTEGRALS ON HARDY TYPE SPACES

In this paper, the authors study the boundedness of the operator [μΩ,b], the commutator generated by a function b ∈ Lipβ(Rn)(0 <β≤1) and the Marcinkiewicz integrals μΩ, on the classical Hardy spaces and the Herz-type Hardy spaces in the case Ω ∈ Lipα(Sn-1)(0 <α≤ 1).

Application of ion chromatography to the determination of water-soluble inorganic and organic ions in atmospheric aerosols

A simple, sensitive and convenient ion chromatography(IC) method was established for the simultaneous determination of twelve water-soluble inorganic anions(F -, Cl -, NO - 2, NO - 3, SO 2- 3, SO 2- 4, PO 3- 4), and fifteen water-soluble organic ions(formate, acetate, MSA, oxalate, malonate, succinate, phthalates, etc.) in atmospheric aerosols. The linear concentrations ranged from 0.005 μg/m 3 to 500 μg/m 3(r = 0.999—0.9999). The relative standard deviation(RSD) were 0.43%—2.00% and the detection limits were from 2.7 ng/m 3 to 88 ng/m 3. The proposed method was successfully applied to the simultaneous determination of those inorganic ions and organic ions in PM 2.5 of Beijing.

A simulation analysis of the migration and transformation of pollutants contained in landfill leachate

A dynamic composite model for a soil-water system that can be used to simulate the movement of leachate from a landfill. The composite model includes nine sub-models that trace water movement and the migration and transformation of five pollutants(organic N, NH - 4, NO - 3, NO - 2, and Cl -) in saturated and unsaturated soil. The model to simulate the movement of leachate from a landfill in Laogang Town, Shanghai City was used. In this application, the values for the model parameters were obtained by performing a laboratory simulation experiment of water movement and pollutant migration and transformation in soil columns. Soil and leachate obtained from the landfill site and its vicinity were used in the laboratory experiments. The model was then used to simulate leachate movement and pollutant activity during the ten-year period when the landfill was in operation and in the twenty-year period following its closure. The simulation results revealed that the leachate migrated into the groundwater at the rate of 90—100 meters per year. This model can be applied in the design of future landfills in China for the purpose of assessing and forecasting leachate plumes.

Unusual Charge Transport and Spin Response of Doped Bilayer Triangular Antiferromagnets

Within the t-J model, the charge transport and spin response of the doped bilayer triangular antiferromagnetare studied by considering the bilayer interaction. Although the bilayer interaction leads to the band splitting in theelectronic structure, the qualitative behaviors of the physical properties are the same as in the single layer case. Theconductivity spectrum shows the low-energy peak and unusual midinfrared band, the temperature-dependent resistivityis characterized by the nonlinearity metallic-like behavior in the higher temperature range and the deviation from themetallic-like behavior in the lower temperature range and the commensurate neutron scattering peak near the half-fillingis split into six incommensurate peaks in the underdoped regime, with the incommensurability increasing with the holeconcentration at lower dopings, and saturating at higher dopings.

Average Cycle Period in Asymmetrical Flashing Ratchet

The directed motion of a Brownian particle in a flashing potential with various transition probabilities and waiting times in one of two states is studied. An expression for the average cycle period is proposed and the steady current J of the particle is calculated via Langevin simulation. The results show that the optimal cycle period rm,which takes the maximum of J, is shifted to a small value when the transition probability λ from the potential on to the potential off decreases, the maximalcurrent appears in the case of the average waiting time in the potential on being longer than in the potential off, and the direction of current depends on the ratio of the average times waiting in two states.

Simulation of astronomical solar radiation over Yellow River Basin based on DEM

Based on the developed distributed model for calculating astronomical solar radiation (ASR), monthly ASR with a resolution of 1 km×1 km for the rugged terrains of Yellow River Basin was calculated, with DEM data as the general characterization of terrain. This model gives an all-sided consideration on factors that influence the ASR. Results suggest that (1) Annual ASR has a progressive decrease trend from south to north; (2) the magnitude order of seasonal ASR is: summer>spring>autumn>winter; (3) topographical factors have robust effect on the spatial distribution of ASR, particularly in winter when a lower sun elevation angle exists; (4) the ASR of slopes with a sunny exposure is generally 2 or 3 times that of slopes with a shading exposure and the extreme difference of ASR for different terrains is over 10 times in January; (5) the spatial differences of ASR are relatively small in summer when a higher sun elevation angle exists and the extreme difference of ASR for different terrains is only 16% in July; and (6) the sequence of topographical influence strength is: winter>autumn>spring>summer.

Thermal Fission Rate Calculated Numerically by Particles Multi-passing over SaddlePoint

Langevin simulation of the particles multi-passing over the saddle point is proposed to calculate thermal fission rate.Due to finite friction and the corresponding thermal flutuation,a backstreaming exists in the process of the particle descent from the saddle to the scissio.This leads to that the diffusion behind the saddle point has influence upon the stationary flow across the saddle point.A dynamical correction factor,as a ratio of the flows of multi-and firstoverpassing the saddle point,is evaluated analytically.The results show that the fission rate calculated by the particles multi-passing over the saddle point is lower than the one calculated by the particle firstly passing over the saddle point,and the fomer approaches the results at the scission point.