AIM: To summarize clinical features of probable severe acute respiratory syndrome (SARS) in Beijing.METHODS: Retrospective cases involving 801 patients admitted to hospitals in Beijing between March and June 2003, wit...AIM: To summarize clinical features of probable severe acute respiratory syndrome (SARS) in Beijing.METHODS: Retrospective cases involving 801 patients admitted to hospitals in Beijing between March and June 2003, with a diagnosis of probable SARS, moderate type.The series of clinical manifestation, laboratory and radiograph data obtained from 801 cases were analyzed. RESULTS: One to three days after the onset of SARS, the major clinical symptoms were fever (in 88.14% of patients), fatigue, headache, myalgia, arthralgia (25-36%), etc. The counts of WBC (in 22.56% of patients) lymphocyte (70.25%)and CD3, CD4, CD8 positive T cells (70%) decreased. From 4-7 d, the unspecific symptoms became weak; however, the rates of low respiratory tract symptoms, such as cough (24.18%), sputum production (14.26%), chest distress (21.04%) and shortness of breath (9.23%) increased, so did the abnormal rates on chest radiograph or CT. The low counts of WBC, lymphocyte and CD3, CD4, CD8 positiveT cells touched bottom. From 8 to 16 d, the patients presented progressive cough (29.96%), sputum production (13.09%), chest distress (29.96%) and shortness of breath (35.34%). All patients had infiltrates on chest radiograph or CT, some even with multi-infiltrates. Two weeks later, patients' respiratory symptoms started to alleviate, the infiltrates on the lung began to absorb gradually, the counts of WBC, lymphocyte and CD3, CD4, CD8 positive T cells were restored to normality.CONCLUSION: The data reported here provide evidence that the course of SARS could be divided into four stages, namely the initial stage, progressive stage, fastigium and convalescent stage.展开更多
Objective To investigate whether the osteocyte apoptosis exists in orbital bones and to discuss its effect on the orbital development. Methods Seven young Newzealand white rabbits were selected as experimental animals...Objective To investigate whether the osteocyte apoptosis exists in orbital bones and to discuss its effect on the orbital development. Methods Seven young Newzealand white rabbits were selected as experimental animals.At two-month-old ,all rabbits were killed and then zygomas were made into paraffin and electron microscope sections after they were decalcified. Apoptosis of osteocytes was observed by light microscope and transmission electron microscopes and detected by TUNEL staining. Results The classical apoptosis of osteocytes was found under light and transmission electron microscopes. Apoptosis of osteocytes was diffused irregularly in the zygomatic tissue. Conclusion Osteocyte can apoptosis and it may participate in the development of the bony orbit.展开更多
Purpose:According to Quigley's hypothesis the dense of connective tissue is the least and the pores of the lamina cribrosa are the largest at the superior and infe-rior poles of the lamina cribrosa,therefore they ...Purpose:According to Quigley's hypothesis the dense of connective tissue is the least and the pores of the lamina cribrosa are the largest at the superior and infe-rior poles of the lamina cribrosa,therefore they are the most vulnerable location to be involved in the characteristic glaucomatous optic nerve damage,To get clin-ical evidence for the hypltheses,the reversal of optic cup in adults glaucoma after reduction of intraocular pressure(IOP)was examined.Methods:The stereoscopic flicker comparison on with computerized image sys-tem was used to monitor the optic cup's changing,2 serial superposed stereo pairs were displayed alternatively and rapidly and the changing parts appeared moving Under the stereoscopic observation ,hthe changes of 3-dimention optic cup could be seen and the false positive phenomena caused by photographic angle variation,vascular pulsation could be differentiated from the characteristic change of the cup.Stereo fundus photographs were taken from 31eyes with hy-pertension glaucoma before and after treatment of reduction of IOPs which was either diamox administration or trabeculectomy.Results:the result showed that the reversal of optic cups after reduction of IOPs were mostly asymmetrical,especially at the inferior and/or superior poles.By multiple stepwise regression.it is known that the amount of the change is only correlated with the initial elevated IOP.Conclusions:The study indicated that it was the distortion and deformity of the lamina cribrosa leading to shearing stress that results in glaucomatous character-istic damage in structure and function.The individul tolerance of the lamina cribrosa to the high intraocular pressure and the locations of indivdual vulnerable at he optic nerve head are various.Eye Science1995;11:155-160.展开更多
Objective To investigate the association between the polymorphism of codon-54 of the fatty acid binding protein 2 (FABP2) gene and patients with type 2 diabetes, and how to infect metabolism of lipoprotein. Methods Th...Objective To investigate the association between the polymorphism of codon-54 of the fatty acid binding protein 2 (FABP2) gene and patients with type 2 diabetes, and how to infect metabolism of lipoprotein. Methods The Ala54Thr variation of FABP2 was detected by PCR and Hae-Ⅱ digestion in 225 Chinese subjects, including 117 cases of type 2 diabetes mellitus and 108 cases of normal controls. All cases were detected for fasting plasma lipoprotein. Results (1)The polymorphism restriction site of codon-54 of FABP2 gene results in the substitution of threonine(Thr) for alanine(Ala). Of the 117 diabetic patients screened, 64 (54.7%) were heterozygous, 32 (27.4%) were homozygous for Ala-54 allele and 21 (17.9%) were homozygous for the Thr-54 allele. (2)The frequence of genotype Ala54/Thr54 and Thr54/Thr54 significiantly increased in the type 2 diabetes as compared with that in healthy subjects (P:0.018).(3)The odds ratio of FABP2 genotype Ala54/Thr54 and Thr54/Thr54 for the patients with type 2 diabetes was 1.97(95% confidence intervals is 1.13-3.44). (4)The frequency of the FABP2 mutant thr54 allele was similar in men and women (33.3% and 32.0%, respectively, P=0.675). (5)The patients with type 2 diabetes who carry Ala54/Thr54 and Thr54/Thr54 genotype had a significantly higher level of fasting plasma triglyceride (P=0.003) and lower level of high density lipoprotein cholesterol (HDL-C) (P=0.001)than those with the wild-type. Conclusions (1)FABP2 gene polymorphism seems to be significantly associated with type 2 diabetes; the codon-54 of mutant genotypic frequencies were in Hardy-Weinberg equilibrium. (2)The FABP2 ala54thr polymorphism appears to have no significant difference in men and women. (3)FABP2 polymorphism may have a certain contribution to the abnormity of lipoprotein metabolism in individuals.展开更多
基金Supported by the National High Technology Research and Development Program of China (863 Program), No. 2003AA208107 Correspondence to: Xiao-Yuan Xu
文摘AIM: To summarize clinical features of probable severe acute respiratory syndrome (SARS) in Beijing.METHODS: Retrospective cases involving 801 patients admitted to hospitals in Beijing between March and June 2003, with a diagnosis of probable SARS, moderate type.The series of clinical manifestation, laboratory and radiograph data obtained from 801 cases were analyzed. RESULTS: One to three days after the onset of SARS, the major clinical symptoms were fever (in 88.14% of patients), fatigue, headache, myalgia, arthralgia (25-36%), etc. The counts of WBC (in 22.56% of patients) lymphocyte (70.25%)and CD3, CD4, CD8 positive T cells (70%) decreased. From 4-7 d, the unspecific symptoms became weak; however, the rates of low respiratory tract symptoms, such as cough (24.18%), sputum production (14.26%), chest distress (21.04%) and shortness of breath (9.23%) increased, so did the abnormal rates on chest radiograph or CT. The low counts of WBC, lymphocyte and CD3, CD4, CD8 positiveT cells touched bottom. From 8 to 16 d, the patients presented progressive cough (29.96%), sputum production (13.09%), chest distress (29.96%) and shortness of breath (35.34%). All patients had infiltrates on chest radiograph or CT, some even with multi-infiltrates. Two weeks later, patients' respiratory symptoms started to alleviate, the infiltrates on the lung began to absorb gradually, the counts of WBC, lymphocyte and CD3, CD4, CD8 positive T cells were restored to normality.CONCLUSION: The data reported here provide evidence that the course of SARS could be divided into four stages, namely the initial stage, progressive stage, fastigium and convalescent stage.
文摘Objective To investigate whether the osteocyte apoptosis exists in orbital bones and to discuss its effect on the orbital development. Methods Seven young Newzealand white rabbits were selected as experimental animals.At two-month-old ,all rabbits were killed and then zygomas were made into paraffin and electron microscope sections after they were decalcified. Apoptosis of osteocytes was observed by light microscope and transmission electron microscopes and detected by TUNEL staining. Results The classical apoptosis of osteocytes was found under light and transmission electron microscopes. Apoptosis of osteocytes was diffused irregularly in the zygomatic tissue. Conclusion Osteocyte can apoptosis and it may participate in the development of the bony orbit.
文摘Purpose:According to Quigley's hypothesis the dense of connective tissue is the least and the pores of the lamina cribrosa are the largest at the superior and infe-rior poles of the lamina cribrosa,therefore they are the most vulnerable location to be involved in the characteristic glaucomatous optic nerve damage,To get clin-ical evidence for the hypltheses,the reversal of optic cup in adults glaucoma after reduction of intraocular pressure(IOP)was examined.Methods:The stereoscopic flicker comparison on with computerized image sys-tem was used to monitor the optic cup's changing,2 serial superposed stereo pairs were displayed alternatively and rapidly and the changing parts appeared moving Under the stereoscopic observation ,hthe changes of 3-dimention optic cup could be seen and the false positive phenomena caused by photographic angle variation,vascular pulsation could be differentiated from the characteristic change of the cup.Stereo fundus photographs were taken from 31eyes with hy-pertension glaucoma before and after treatment of reduction of IOPs which was either diamox administration or trabeculectomy.Results:the result showed that the reversal of optic cups after reduction of IOPs were mostly asymmetrical,especially at the inferior and/or superior poles.By multiple stepwise regression.it is known that the amount of the change is only correlated with the initial elevated IOP.Conclusions:The study indicated that it was the distortion and deformity of the lamina cribrosa leading to shearing stress that results in glaucomatous character-istic damage in structure and function.The individul tolerance of the lamina cribrosa to the high intraocular pressure and the locations of indivdual vulnerable at he optic nerve head are various.Eye Science1995;11:155-160.
文摘Objective To investigate the association between the polymorphism of codon-54 of the fatty acid binding protein 2 (FABP2) gene and patients with type 2 diabetes, and how to infect metabolism of lipoprotein. Methods The Ala54Thr variation of FABP2 was detected by PCR and Hae-Ⅱ digestion in 225 Chinese subjects, including 117 cases of type 2 diabetes mellitus and 108 cases of normal controls. All cases were detected for fasting plasma lipoprotein. Results (1)The polymorphism restriction site of codon-54 of FABP2 gene results in the substitution of threonine(Thr) for alanine(Ala). Of the 117 diabetic patients screened, 64 (54.7%) were heterozygous, 32 (27.4%) were homozygous for Ala-54 allele and 21 (17.9%) were homozygous for the Thr-54 allele. (2)The frequence of genotype Ala54/Thr54 and Thr54/Thr54 significiantly increased in the type 2 diabetes as compared with that in healthy subjects (P:0.018).(3)The odds ratio of FABP2 genotype Ala54/Thr54 and Thr54/Thr54 for the patients with type 2 diabetes was 1.97(95% confidence intervals is 1.13-3.44). (4)The frequency of the FABP2 mutant thr54 allele was similar in men and women (33.3% and 32.0%, respectively, P=0.675). (5)The patients with type 2 diabetes who carry Ala54/Thr54 and Thr54/Thr54 genotype had a significantly higher level of fasting plasma triglyceride (P=0.003) and lower level of high density lipoprotein cholesterol (HDL-C) (P=0.001)than those with the wild-type. Conclusions (1)FABP2 gene polymorphism seems to be significantly associated with type 2 diabetes; the codon-54 of mutant genotypic frequencies were in Hardy-Weinberg equilibrium. (2)The FABP2 ala54thr polymorphism appears to have no significant difference in men and women. (3)FABP2 polymorphism may have a certain contribution to the abnormity of lipoprotein metabolism in individuals.
