In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis...In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis, the injured radial nerve was enwrapped with the prepared nerve conduit, which was fixed to the epineurium by sutures, with the cell on the inner surface of the conduit. Simultaneously, a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals. A total of 1.75 x 107 cells were seeded on the amniotic membrane. In the control group, patients received only neurolysis. At 12 weeks after cell transplantation, more than 80% of patients exhibited obvious improvements in muscular strength, and touch and pain sensations. In contrast, these improvements were observed only in 55-65% of control patients. At 8 and 12 weeks, muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group. After cell transplantation, no immunological rejections were observed. These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury.展开更多
The effect of Panaxatriol Ginsenoside (PTGS) on Immune functions in bone marrow suppressed mice induced by injection of cyclophosphamide (CY) has been studied. Bone marrow suppressed mice were made by injection of CY ...The effect of Panaxatriol Ginsenoside (PTGS) on Immune functions in bone marrow suppressed mice induced by injection of cyclophosphamide (CY) has been studied. Bone marrow suppressed mice were made by injection of CY (150 mg/kg) parenterally. Subcutaneous injection of PTGS three days earlier partially restored the number and the activity of bone marrow cells, significantly enhanced the production of IL-1, IL- 3 and IL- 6 like substances and promoted the reactivity of murlne spleen cells to Con-A In bone marrow suppressed mice.展开更多
By means of electron paramagnetic resonance(EPR), chemistry luminescence and fluorescent spectroscopy, the free radical, superoxide dismutase and water soluble lipid peroxide in tears of normal eyes(150 eyes, 100 case...By means of electron paramagnetic resonance(EPR), chemistry luminescence and fluorescent spectroscopy, the free radical, superoxide dismutase and water soluble lipid peroxide in tears of normal eyes(150 eyes, 100 cases), Moorens ulcer(9 eyes), coreal grafts rejection(16 eyes) were studied. The results showed that the spin density of the free radical was 36(±058)×1012 spins/mL tear, the content of the superoxide dismutase(SOD) was 384(±145) ng/mL tear, the opposite fluorescent density of the water soluble lipid peroxide was 12912(±1691) U/mL tear in normal tears. The normal values are 25—48×1012 spins/mL tear for free radical, 239—529 ng/mL tear for SOD, 9598—16225 U/mL tear for water soluble lipid peroxide. There are significant differences in different eyes, different sexes and different ages. The free radical and lipid peroxide are higher obviously in the tears of patients with Moorens ulcer and rejected corneal grafts, compared with those of the normal control subjects(P<001), SOD is lower evidently(P<001). The above fact shows the pathogenic mechanism of Moorens ulcer and keractoplasty rejection is significantly related to toxicities injuring effect of the free radical. These results have provided important experimental data for studying lacrimalogy and new methods for clinical diagnosis and treatrment.展开更多
Duck hepatitis B vims (DHBV) DNA was detected in different tumorous nodules of ducks with hepatic multicentric cancer or intrahepatic metastasis by Southern blot technique. Among 7 ducks with hepatocellular carcinoma ...Duck hepatitis B vims (DHBV) DNA was detected in different tumorous nodules of ducks with hepatic multicentric cancer or intrahepatic metastasis by Southern blot technique. Among 7 ducks with hepatocellular carcinoma of multiple tumor nodules, the hybridization pattern of Integrated DHBV DNA In different tumorous nodules was identical in 3 cases and different in 2 cases. One case showed a similar hybridization pattern in two tumorous nodules and other one was negative tor DHBV DNA. Integrated DHBV DNA was also identified in a metastatic lung cancer of ducks with hepatocellular carcinoma. The hybridization pattern of metastasis of lungs was as the some as that in primary hepatocellular carcinoma. The same discrete hybridization bands In the different tumorous nodules indicate that these nodules might arise from one transformed cell. The different hybridization patterns In various tumorous nodules show that these tumorous nodules might arise from various transformed cells. The results suggest that the hybridization pattern of different nodules of hepatocellular carcinoma with viral DNA probe could make a cell clone origin marker of tumor nodule to differentiate hepatic multlcentric cancer from Intrahepatic metastatic cancer.展开更多
Four human vascular endothelial growth factor receptor Flt-1 cDNA fragments containing extracellular domain loops 2, 1-2, 2-3 and 1-3 respectively were amplified from human placen-tal cDNA library by PCR and used for ...Four human vascular endothelial growth factor receptor Flt-1 cDNA fragments containing extracellular domain loops 2, 1-2, 2-3 and 1-3 respectively were amplified from human placen-tal cDNA library by PCR and used for screening ligand binding domains by yeast two-hybrid system. The result showed that, not only loop 1-3, but also the smaller fragment loop 2-3 could bind to hVEGF165. Recombinant expression plasmids pPIC9K/Flt-1(1-3) and pPIC9K/Flt-1 (2-3) were constructed and transformed to Pichia. pastoris host strain GS115, cultured in flasks, and expressed under the induction of 1 % methanol. The expressed product existed in supernatant in the form of soluble molecules and contained more than 60% of total protein after being induced for 4d. After being purified by CM-Sepharose FF and Sephacryl S-100 chromatography, its purity reached above 90%. Biological assay in vitro showed that the binding capacity of expressed soluble Flt-1 (2-3) to hVEGF165 and its inhibiting effect on the proliferation of human umbilical veins endothelial cells (HUVEC) stimulated with hVEGF165 were close to those of sFlt-1(1-3). Animal test showed that sFlt-1(2-3) could inhibit the formation of regenerate blood vessels stimulated with hVEGF165 significantly.展开更多
The roots of oplopanax elatus NAKAI (Chinese name, Cirensen) (Araliaceae) have been used as a medicine in China for tonica,antitussis, eliminating fever,regulating blood pressure and so on.The chemical studies of the ...The roots of oplopanax elatus NAKAI (Chinese name, Cirensen) (Araliaceae) have been used as a medicine in China for tonica,antitussis, eliminating fever,regulating blood pressure and so on.The chemical studies of the leaves of this plant were reported previously.The present note describes the isolation and展开更多
The rare earth elements at large dose are poisonous to organisms. The mechanism of the poisoning may be complex. But the rare earth elements poisoning as well as some other metal poisonings (e. g. lead, gold and mercu...The rare earth elements at large dose are poisonous to organisms. The mechanism of the poisoning may be complex. But the rare earth elements poisoning as well as some other metal poisonings (e. g. lead, gold and mercury) exhibit a common feature——to deposit in bodies. The rare earth elements, from lanthanum to samarium (Sm)which were injected in-展开更多
Ginseng fruit is the fully grown berries of the famous medicinal plant Panax ginseng C. A. Meyer. In recent years, it has been found that all the saponins the ginseng fruit contains are effective in resisting fatigue,...Ginseng fruit is the fully grown berries of the famous medicinal plant Panax ginseng C. A. Meyer. In recent years, it has been found that all the saponins the ginseng fruit contains are effective in resisting fatigue, reducing symptoms of oxygen deficit and preventing arteriosclerosis caused by excessive amount of cholesterol in the blood. So the dammarane-type saponins of the ginseng fruit have been studied. Eight such monomer展开更多
Neuropeptide Y(NPY) is a 36-amino acid polypeptide which distributes in many important cardiovascular centers, and is involved in cardiovascular regulation. The new evidence indicates that NPY and the receptor of NPY ...Neuropeptide Y(NPY) is a 36-amino acid polypeptide which distributes in many important cardiovascular centers, and is involved in cardiovascular regulation. The new evidence indicates that NPY and the receptor of NPY exist in the field CA3 of the rat hippocampus, but cardiovascular effect of NPY on CA3 of hippocampus is still unknown. We have studied the cardiovascular effect on CA3 in the anaesthetized Wistar rats injected with NPY.Among 6 rats, the basal levels of mean arterial pressure (MAP) and heart rate (HR)展开更多
Atrial natriuretic factor (ANF)is a peptide hormone originally from cardiac atria. The human ANF gene is located on the distal short arm of chromosome 1, about 2.1 Kbp in length. It is coded prepro-ANF with 151 amino ...Atrial natriuretic factor (ANF)is a peptide hormone originally from cardiac atria. The human ANF gene is located on the distal short arm of chromosome 1, about 2.1 Kbp in length. It is coded prepro-ANF with 151 amino acids, the 28 amino acid residue fragment in C terminal of prepro-ANF is thus called ANF. ANF gene expression is significant in maintenance of humoral homeostasis and regulation of cardiovascular function. To study how the ANF gene expresses and if there is a regular pattern during ontogenic development展开更多
Panaxatriol ginsenoside (PTGS) is the major element of ginsenoside. It was shown that ginsenoside could regulate the functions of several immune cells, possibly mediated by enhanced secretion of interleukine-2 (IL-2) ...Panaxatriol ginsenoside (PTGS) is the major element of ginsenoside. It was shown that ginsenoside could regulate the functions of several immune cells, possibly mediated by enhanced secretion of interleukine-2 (IL-2) and interferon (IFN) . Recently, it was shown that the promotion of cytokines (IL-1, 2, 3, 4, 5, 6, IFN)with PTGS was related展开更多
Membrane distillation is a new membrane separation process which has been developed in the last few years. When a piece of microporous hydrophobic membrane separates two kinds of aqueous solutions different in tempera...Membrane distillation is a new membrane separation process which has been developed in the last few years. When a piece of microporous hydrophobic membrane separates two kinds of aqueous solutions different in temperature, the solutions cannot transport through the pores of membrane in any directions because of the hydrophobicity of membrane. However, vapor can readily penetrate through展开更多
Human alpha-fetoprotein (AFP) is a glycoprotein produced by fetal liver and yolk sac. It has attracted great attention because of its increasing amount in haptocellular
Atrial natriuretic peptide (ANP) is a kind of peptide hormone isolated and purified originally from mammalian heart atria, which possesses potent natriuretic, diuretic and vasorelaxant actions. So far it was further n...Atrial natriuretic peptide (ANP) is a kind of peptide hormone isolated and purified originally from mammalian heart atria, which possesses potent natriuretic, diuretic and vasorelaxant actions. So far it was further needed to elucidate the factors affecting ANP gene expression, ANP synthesis and secretion etc., especially the knowledge about ANP in brain展开更多
基金the Science and Technology Foundation of Shenyang in China,No.F10-217-1-00
文摘In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis, the injured radial nerve was enwrapped with the prepared nerve conduit, which was fixed to the epineurium by sutures, with the cell on the inner surface of the conduit. Simultaneously, a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals. A total of 1.75 x 107 cells were seeded on the amniotic membrane. In the control group, patients received only neurolysis. At 12 weeks after cell transplantation, more than 80% of patients exhibited obvious improvements in muscular strength, and touch and pain sensations. In contrast, these improvements were observed only in 55-65% of control patients. At 8 and 12 weeks, muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group. After cell transplantation, no immunological rejections were observed. These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury.
文摘The effect of Panaxatriol Ginsenoside (PTGS) on Immune functions in bone marrow suppressed mice induced by injection of cyclophosphamide (CY) has been studied. Bone marrow suppressed mice were made by injection of CY (150 mg/kg) parenterally. Subcutaneous injection of PTGS three days earlier partially restored the number and the activity of bone marrow cells, significantly enhanced the production of IL-1, IL- 3 and IL- 6 like substances and promoted the reactivity of murlne spleen cells to Con-A In bone marrow suppressed mice.
文摘By means of electron paramagnetic resonance(EPR), chemistry luminescence and fluorescent spectroscopy, the free radical, superoxide dismutase and water soluble lipid peroxide in tears of normal eyes(150 eyes, 100 cases), Moorens ulcer(9 eyes), coreal grafts rejection(16 eyes) were studied. The results showed that the spin density of the free radical was 36(±058)×1012 spins/mL tear, the content of the superoxide dismutase(SOD) was 384(±145) ng/mL tear, the opposite fluorescent density of the water soluble lipid peroxide was 12912(±1691) U/mL tear in normal tears. The normal values are 25—48×1012 spins/mL tear for free radical, 239—529 ng/mL tear for SOD, 9598—16225 U/mL tear for water soluble lipid peroxide. There are significant differences in different eyes, different sexes and different ages. The free radical and lipid peroxide are higher obviously in the tears of patients with Moorens ulcer and rejected corneal grafts, compared with those of the normal control subjects(P<001), SOD is lower evidently(P<001). The above fact shows the pathogenic mechanism of Moorens ulcer and keractoplasty rejection is significantly related to toxicities injuring effect of the free radical. These results have provided important experimental data for studying lacrimalogy and new methods for clinical diagnosis and treatrment.
文摘Duck hepatitis B vims (DHBV) DNA was detected in different tumorous nodules of ducks with hepatic multicentric cancer or intrahepatic metastasis by Southern blot technique. Among 7 ducks with hepatocellular carcinoma of multiple tumor nodules, the hybridization pattern of Integrated DHBV DNA In different tumorous nodules was identical in 3 cases and different in 2 cases. One case showed a similar hybridization pattern in two tumorous nodules and other one was negative tor DHBV DNA. Integrated DHBV DNA was also identified in a metastatic lung cancer of ducks with hepatocellular carcinoma. The hybridization pattern of metastasis of lungs was as the some as that in primary hepatocellular carcinoma. The same discrete hybridization bands In the different tumorous nodules indicate that these nodules might arise from one transformed cell. The different hybridization patterns In various tumorous nodules show that these tumorous nodules might arise from various transformed cells. The results suggest that the hybridization pattern of different nodules of hepatocellular carcinoma with viral DNA probe could make a cell clone origin marker of tumor nodule to differentiate hepatic multlcentric cancer from Intrahepatic metastatic cancer.
基金National High Technology Programs of China (Grant No. 102-08-01-03) and Natural Science Fund Program of Guangdong Province (Grant No.001098).
文摘Four human vascular endothelial growth factor receptor Flt-1 cDNA fragments containing extracellular domain loops 2, 1-2, 2-3 and 1-3 respectively were amplified from human placen-tal cDNA library by PCR and used for screening ligand binding domains by yeast two-hybrid system. The result showed that, not only loop 1-3, but also the smaller fragment loop 2-3 could bind to hVEGF165. Recombinant expression plasmids pPIC9K/Flt-1(1-3) and pPIC9K/Flt-1 (2-3) were constructed and transformed to Pichia. pastoris host strain GS115, cultured in flasks, and expressed under the induction of 1 % methanol. The expressed product existed in supernatant in the form of soluble molecules and contained more than 60% of total protein after being induced for 4d. After being purified by CM-Sepharose FF and Sephacryl S-100 chromatography, its purity reached above 90%. Biological assay in vitro showed that the binding capacity of expressed soluble Flt-1 (2-3) to hVEGF165 and its inhibiting effect on the proliferation of human umbilical veins endothelial cells (HUVEC) stimulated with hVEGF165 were close to those of sFlt-1(1-3). Animal test showed that sFlt-1(2-3) could inhibit the formation of regenerate blood vessels stimulated with hVEGF165 significantly.
基金Project supported by the National Natural Science Foundation of China.
文摘The roots of oplopanax elatus NAKAI (Chinese name, Cirensen) (Araliaceae) have been used as a medicine in China for tonica,antitussis, eliminating fever,regulating blood pressure and so on.The chemical studies of the leaves of this plant were reported previously.The present note describes the isolation and
基金Project supported by the National Natural Science Foundation of China.
文摘The rare earth elements at large dose are poisonous to organisms. The mechanism of the poisoning may be complex. But the rare earth elements poisoning as well as some other metal poisonings (e. g. lead, gold and mercury) exhibit a common feature——to deposit in bodies. The rare earth elements, from lanthanum to samarium (Sm)which were injected in-
文摘Ginseng fruit is the fully grown berries of the famous medicinal plant Panax ginseng C. A. Meyer. In recent years, it has been found that all the saponins the ginseng fruit contains are effective in resisting fatigue, reducing symptoms of oxygen deficit and preventing arteriosclerosis caused by excessive amount of cholesterol in the blood. So the dammarane-type saponins of the ginseng fruit have been studied. Eight such monomer
基金Project supported by the National Natural Science Foundation of China.
文摘Neuropeptide Y(NPY) is a 36-amino acid polypeptide which distributes in many important cardiovascular centers, and is involved in cardiovascular regulation. The new evidence indicates that NPY and the receptor of NPY exist in the field CA3 of the rat hippocampus, but cardiovascular effect of NPY on CA3 of hippocampus is still unknown. We have studied the cardiovascular effect on CA3 in the anaesthetized Wistar rats injected with NPY.Among 6 rats, the basal levels of mean arterial pressure (MAP) and heart rate (HR)
基金Project supported by the National Natural Science Foundation of China
文摘Atrial natriuretic factor (ANF)is a peptide hormone originally from cardiac atria. The human ANF gene is located on the distal short arm of chromosome 1, about 2.1 Kbp in length. It is coded prepro-ANF with 151 amino acids, the 28 amino acid residue fragment in C terminal of prepro-ANF is thus called ANF. ANF gene expression is significant in maintenance of humoral homeostasis and regulation of cardiovascular function. To study how the ANF gene expresses and if there is a regular pattern during ontogenic development
文摘Panaxatriol ginsenoside (PTGS) is the major element of ginsenoside. It was shown that ginsenoside could regulate the functions of several immune cells, possibly mediated by enhanced secretion of interleukine-2 (IL-2) and interferon (IFN) . Recently, it was shown that the promotion of cytokines (IL-1, 2, 3, 4, 5, 6, IFN)with PTGS was related
文摘Membrane distillation is a new membrane separation process which has been developed in the last few years. When a piece of microporous hydrophobic membrane separates two kinds of aqueous solutions different in temperature, the solutions cannot transport through the pores of membrane in any directions because of the hydrophobicity of membrane. However, vapor can readily penetrate through
文摘Human alpha-fetoprotein (AFP) is a glycoprotein produced by fetal liver and yolk sac. It has attracted great attention because of its increasing amount in haptocellular
基金Project supported by the National Natural Science Foundation of China.
文摘Atrial natriuretic peptide (ANP) is a kind of peptide hormone isolated and purified originally from mammalian heart atria, which possesses potent natriuretic, diuretic and vasorelaxant actions. So far it was further needed to elucidate the factors affecting ANP gene expression, ANP synthesis and secretion etc., especially the knowledge about ANP in brain
