AIM: To investigate the effects of Chinese herb YiganDecoction on proliferation and apoptosis of the hepaticstellafe cells (HSC) in vitro.METHODS: The study in vitro was carried out in the cultureof HSC lines. Various...AIM: To investigate the effects of Chinese herb YiganDecoction on proliferation and apoptosis of the hepaticstellafe cells (HSC) in vitro.METHODS: The study in vitro was carried out in the cultureof HSC lines. Various concentrations of Yigan Decoctionwere added and incubated. Cell proliferation was detectedwith MTT colorimetric assay. Cell apoptosis was detected byelectron microscopy, flow cytometry and TUNEL.RESULTS: The proliferation of HSC was inhibited by YiganDecoction, which depending on dose and time significantly.The HSC proliferation rates ofgroups at the endconoentrations 144 and 72 (g@L-1 ) were 21.62 % and 140.54 %respectively, significantly lower then that of normal controlgroup( P ＜ 0.01 ). The HSC proliferation rates of groups atthe end concentratiors 36, 18 and 9(g@L-1 ) were 54.05 %,45.95 % and 51.35 % respectively, lower than that of controlgroup( P ＜ 0.05). When the end concentration was 4.5 g@L-1, the proliferation rate was 83.78 %, which appeared nosignificant differences compared with control group. At thesame concentrations of 18 g@L-1, the inhibitory effects ofYigan Decoction at 24 h, 48 h and 72 h time point wereobserved, the effects were time-dependent, and reached apeak at 72 h. Meanwhile, it was showed that the inducingeffects of Yigan Decoction on HSC apoptosis were dose-dependent and time-dependent. The apoptosis index (Al)was detected by TUNEL. After Yigan Decoction had beenincubated for 48 h at the end concentration of 18 g@ L-1 , tieAl (14.5 + 3. 1 ) % was significantly higher than that ofcontrol group (4.3+ 1.3) % (P＜0.01). When visualizedunder transnission electron microscopy, some apoptoticsfellafe cells were found, i. e. dilated endoplasmicreticulum, irregular ntclei, chromatin condensation andheterochromatin ranked along inside of nuclear membrane.By flow cytometry detection, after HSC was treated withYigan Decoction at different concentrations of 36, 18 and 9(g@ L-1 ) for 48 h, Al ( % ) were 13.3 ± 3.2, 10.7 ± 2.7 and 10.1 ±2.5 respectively, which were significantly higher than that ofcontrol group(4. 1 ± 1.9) (P ＜ 0.01). At the sameconcentration of 18g@ L-1 for 24h, 48h and 72h, Al (%) were9.3± 1.8、10.7± 2.7 and 14.6±4.3 respectively, which weresignificantly higher than that of control group ( P＜ 0.01).CONCLUSION: Yigan Decoction could significantly inhibitHSC proliferation and increase the apoptosis index of HSCdosedependently and time-dependently, which may berelated to its mechanism of antifibrosis.展开更多
Enzymatic activities of acid phosphatasc, alkaline phosphatase, ATPase, SDHase,LDHase and PAS stain in the head-body part of mouse epididymis were investigated. The head-body part was divided into seven segments. Segm...Enzymatic activities of acid phosphatasc, alkaline phosphatase, ATPase, SDHase,LDHase and PAS stain in the head-body part of mouse epididymis were investigated. The head-body part was divided into seven segments. Segment I was composed of ductuli展开更多
An observation was performed on ovary and uterus endometrium of female mice after administration of a syrup containing root extract of Tripterygium wilfordii Hook.F,( TWH ) for 6 months with light and electron micosco...An observation was performed on ovary and uterus endometrium of female mice after administration of a syrup containing root extract of Tripterygium wilfordii Hook.F,( TWH ) for 6 months with light and electron micoscopics. Ovaries were enlarged for展开更多
Effects of Tripterypium Wilfordii Hook f (TWH) on sperm atozoa in the epi- didym is and splenic NK cells activity in m ale m ice w ere observed using MTT assay and silver impregnation m ethods. The results show ed t...Effects of Tripterypium Wilfordii Hook f (TWH) on sperm atozoa in the epi- didym is and splenic NK cells activity in m ale m ice w ere observed using MTT assay and silver impregnation m ethods. The results show ed that the density, viability and m otility of the epididym alsperm atozoa in the experim entalgroupstreated w ith TWH w ere m ore significantly reduced than those in the controlgroup (P< 0.01). The head sw elling, head separation from tailin the groups treated w ith TWH w ere observed. The inhibition of splenicNK cellsactivity in m iceby TWH w asdose-dependent. Inhi- bition by TⅡand TWH athigh dose on the NK cells activity w as significant (P< 0.01 and P< 0.05), w hileinhibitory effectsof TWH atinterm ediateand low doseson the NK cells activity w ere notobserved (P> 0.05). Itw as concluded thatTWH at low er antifertility dose did not significantly inhibit the splenic NK cells activity. It m ightbe usefulforevaluating thetherapeuticeffectsof TWH in futureclinicalprac- tice.展开更多
基金Hebei Province Administration Bureau of TCM,No.200001
文摘AIM: To investigate the effects of Chinese herb YiganDecoction on proliferation and apoptosis of the hepaticstellafe cells (HSC) in vitro.METHODS: The study in vitro was carried out in the cultureof HSC lines. Various concentrations of Yigan Decoctionwere added and incubated. Cell proliferation was detectedwith MTT colorimetric assay. Cell apoptosis was detected byelectron microscopy, flow cytometry and TUNEL.RESULTS: The proliferation of HSC was inhibited by YiganDecoction, which depending on dose and time significantly.The HSC proliferation rates ofgroups at the endconoentrations 144 and 72 (g@L-1 ) were 21.62 % and 140.54 %respectively, significantly lower then that of normal controlgroup( P ＜ 0.01 ). The HSC proliferation rates of groups atthe end concentratiors 36, 18 and 9(g@L-1 ) were 54.05 %,45.95 % and 51.35 % respectively, lower than that of controlgroup( P ＜ 0.05). When the end concentration was 4.5 g@L-1, the proliferation rate was 83.78 %, which appeared nosignificant differences compared with control group. At thesame concentrations of 18 g@L-1, the inhibitory effects ofYigan Decoction at 24 h, 48 h and 72 h time point wereobserved, the effects were time-dependent, and reached apeak at 72 h. Meanwhile, it was showed that the inducingeffects of Yigan Decoction on HSC apoptosis were dose-dependent and time-dependent. The apoptosis index (Al)was detected by TUNEL. After Yigan Decoction had beenincubated for 48 h at the end concentration of 18 g@ L-1 , tieAl (14.5 + 3. 1 ) % was significantly higher than that ofcontrol group (4.3+ 1.3) % (P＜0.01). When visualizedunder transnission electron microscopy, some apoptoticsfellafe cells were found, i. e. dilated endoplasmicreticulum, irregular ntclei, chromatin condensation andheterochromatin ranked along inside of nuclear membrane.By flow cytometry detection, after HSC was treated withYigan Decoction at different concentrations of 36, 18 and 9(g@ L-1 ) for 48 h, Al ( % ) were 13.3 ± 3.2, 10.7 ± 2.7 and 10.1 ±2.5 respectively, which were significantly higher than that ofcontrol group(4. 1 ± 1.9) (P ＜ 0.01). At the sameconcentration of 18g@ L-1 for 24h, 48h and 72h, Al (%) were9.3± 1.8、10.7± 2.7 and 14.6±4.3 respectively, which weresignificantly higher than that of control group ( P＜ 0.01).CONCLUSION: Yigan Decoction could significantly inhibitHSC proliferation and increase the apoptosis index of HSCdosedependently and time-dependently, which may berelated to its mechanism of antifibrosis.
文摘Enzymatic activities of acid phosphatasc, alkaline phosphatase, ATPase, SDHase,LDHase and PAS stain in the head-body part of mouse epididymis were investigated. The head-body part was divided into seven segments. Segment I was composed of ductuli
文摘An observation was performed on ovary and uterus endometrium of female mice after administration of a syrup containing root extract of Tripterygium wilfordii Hook.F,( TWH ) for 6 months with light and electron micoscopics. Ovaries were enlarged for
文摘Effects of Tripterypium Wilfordii Hook f (TWH) on sperm atozoa in the epi- didym is and splenic NK cells activity in m ale m ice w ere observed using MTT assay and silver impregnation m ethods. The results show ed that the density, viability and m otility of the epididym alsperm atozoa in the experim entalgroupstreated w ith TWH w ere m ore significantly reduced than those in the controlgroup (P< 0.01). The head sw elling, head separation from tailin the groups treated w ith TWH w ere observed. The inhibition of splenicNK cellsactivity in m iceby TWH w asdose-dependent. Inhi- bition by TⅡand TWH athigh dose on the NK cells activity w as significant (P< 0.01 and P< 0.05), w hileinhibitory effectsof TWH atinterm ediateand low doseson the NK cells activity w ere notobserved (P> 0.05). Itw as concluded thatTWH at low er antifertility dose did not significantly inhibit the splenic NK cells activity. It m ightbe usefulforevaluating thetherapeuticeffectsof TWH in futureclinicalprac- tice.
