Endothelial progenitor cells secrete a variety of growth factors that inhibit inflammation, promote angiogenesis and exert neuroprotective effects. Therefore, in this study, we investigated whether endothelial progeni...Endothelial progenitor cells secrete a variety of growth factors that inhibit inflammation, promote angiogenesis and exert neuroprotective effects. Therefore, in this study, we investigated whether endothelial progenitor cell-conditioned medium might have therapeutic effectiveness for the treatment of spinal cord injury using both in vitro and in vivo experiments. After primary culture of bone marrow-derived macrophages, lipopolysaccharide stimulation was used to classically activate macrophages to their proinflammatory phenotype. These cells were then treated with endothelial progenitor cell-conditioned medium or control medium. Polymerase chain reaction was used to determine mR NA expression levels of related inflammatory factors. Afterwards, primary cultures of rat spinal cord neuronal cells were prepared and treated with H2O2and either endothelial progenitor cell-conditioned medium or control medium. Hoechst 33258 and propidium iodide staining were used to calculate the proportion of neurons undergoing apoptosis. Aortic ring assay was performed to assess the effect of endothelial progenitor cell-conditioned medium on angiogenesis. Compared with control medium, endothelial progenitor cell-conditioned medium mitigated the macrophage inflammatory response at the spinal cord injury site, suppressed apoptosis, and promoted angiogenesis. Next, we used a rat model of spinal cord injury to examine the effects of the endothelial progenitor cell-conditioned medium in vivo. The rats were randomly administered intraperitoneal injection of PBS, control medium or endothelial progenitor cell-conditioned medium, once a day, for 6 consecutive weeks. Immunohistochemistry was used to observe neuronal morphology. Terminal deoxynucleotidyl transferase-mediated d UTP nick-end labeling assay was performed to detect the proportion of apoptotic neurons in the gray matter. The Basso, Beattie and Bresnahan Locomotor Rating Scale was used to evaluate the recovery of motor function of the bilateral hind limbs after spinal cord injury. Compared with the other two groups, the number of axons was increased, cavities in the spinal cord were decreased, the proportion of apoptotic neurons in the gray matter was reduced, and the Basso, Beattie and Bresnahan score was higher in the endothelial progenitor cell-conditioned medium group. Taken together, the in vivo and in vitro results suggest that endothelial progenitor cell-conditioned medium suppresses inflammation, promotes angiogenesis, provides neuroprotection, and promotes functional recovery after spinal cord injury.展开更多
The eftects of various types of waves on vertical plane turbulent jets are studied numerically in the paper. A ttn＇ee- dimensional numerical model in a-coordinate is developed to study these problems by use of large ...The eftects of various types of waves on vertical plane turbulent jets are studied numerically in the paper. A ttn＇ee- dimensional numerical model in a-coordinate is developed to study these problems by use of large eddy simulation method. Turbulence is modeled by a dynamic coherent eddy model. Numerical results including the distribution of veloci- ty, the decay law of the mean velocity along axis, the turbulent Reynolds stresses and the volume flux per unit width without wave, in the first-order Stokes waves, in the second-order Stokes waves, in the fifth-order Stokes waves, in the solitary waves and in random waves are compared and analyzed. A focus on coherent structures, probability density func- tions and correlation functions of jets is also investigated. The numerical results are of great theoretical importance for un- derstanding jet turbulent behaviors in different types of waves.展开更多
The effect of random waves on vertical plane turbulent jets is studied numerically and the mechanism behind the interaction of the jet and waves is analyzed. The large eddy simulation method is used and the σ-coordin...The effect of random waves on vertical plane turbulent jets is studied numerically and the mechanism behind the interaction of the jet and waves is analyzed. The large eddy simulation method is used and the σ-coordinate system is adopted. Turbulence is modeled by a dynamic coherent eddy model. The σ-coordinate transformation is introduced to map the irregular physical domain with a wavy free surface and an uneven bottom onto a regular computational domain. The fractional step method is used to solve the filtered Navier–Stokes equations. Results presented include the distribution of velocity, the decay law of the mean velocity along the jet axis, self-similar characteristics and volume flux per unit width. In particular, the role of coherent structures on the momentum transfer along the jet centerline and the jet instantaneous characteristics in JONSWAP waves are a special focus of this research. The numerical results obtained are of great theoretical importance in understanding the behavior of turbulent jets in random wave environments.展开更多
The ability of a wet swale,constructed in an area of poor soil permeability,to manage runoff from a roadway was monitored through 27 storm events over a period of 8 months.During the monitoring period,the wet swale re...The ability of a wet swale,constructed in an area of poor soil permeability,to manage runoff from a roadway was monitored through 27 storm events over a period of 8 months.During the monitoring period,the wet swale reduced the total runoff volume by 50.4%through exfiltration and evapotranspiration.The wet swale significantly decreased the influent pollutant concentrations,and the effluent mean concentrations of total suspended solids,total phosphorus,chemical oxygen demand,ammonium,oxidized nitrogen,and total nitrogen in the effluent were 31 mg/L,0.10 mg/L,29 mg/L,0.52 mg/L,0.35 mg/L and1.28 mg/L,respectively.Pollutant loads were also substantially reduced from 70%to 85%.Plant uptake played an important role in nutrient removal in the wet swale.Approximately half of the nitrogen(53.8%)and phosphorus(51.5%)that entered the wet swale was incorporated in above-ground plants.It is shown that wet swales are useful for managing runoff from roads in areas of poor soil permeability.展开更多
基金supported by the National Natural Science Foundation of China,No.81171173 and 81672161
文摘Endothelial progenitor cells secrete a variety of growth factors that inhibit inflammation, promote angiogenesis and exert neuroprotective effects. Therefore, in this study, we investigated whether endothelial progenitor cell-conditioned medium might have therapeutic effectiveness for the treatment of spinal cord injury using both in vitro and in vivo experiments. After primary culture of bone marrow-derived macrophages, lipopolysaccharide stimulation was used to classically activate macrophages to their proinflammatory phenotype. These cells were then treated with endothelial progenitor cell-conditioned medium or control medium. Polymerase chain reaction was used to determine mR NA expression levels of related inflammatory factors. Afterwards, primary cultures of rat spinal cord neuronal cells were prepared and treated with H2O2and either endothelial progenitor cell-conditioned medium or control medium. Hoechst 33258 and propidium iodide staining were used to calculate the proportion of neurons undergoing apoptosis. Aortic ring assay was performed to assess the effect of endothelial progenitor cell-conditioned medium on angiogenesis. Compared with control medium, endothelial progenitor cell-conditioned medium mitigated the macrophage inflammatory response at the spinal cord injury site, suppressed apoptosis, and promoted angiogenesis. Next, we used a rat model of spinal cord injury to examine the effects of the endothelial progenitor cell-conditioned medium in vivo. The rats were randomly administered intraperitoneal injection of PBS, control medium or endothelial progenitor cell-conditioned medium, once a day, for 6 consecutive weeks. Immunohistochemistry was used to observe neuronal morphology. Terminal deoxynucleotidyl transferase-mediated d UTP nick-end labeling assay was performed to detect the proportion of apoptotic neurons in the gray matter. The Basso, Beattie and Bresnahan Locomotor Rating Scale was used to evaluate the recovery of motor function of the bilateral hind limbs after spinal cord injury. Compared with the other two groups, the number of axons was increased, cavities in the spinal cord were decreased, the proportion of apoptotic neurons in the gray matter was reduced, and the Basso, Beattie and Bresnahan score was higher in the endothelial progenitor cell-conditioned medium group. Taken together, the in vivo and in vitro results suggest that endothelial progenitor cell-conditioned medium suppresses inflammation, promotes angiogenesis, provides neuroprotection, and promotes functional recovery after spinal cord injury.
基金supported by the National Key Technology R&D Program of China ( Grant No.2008BAB29B09)the Ph. D. Programs Foundation of the Ministry of Education of China ( Grant No.20070294012)+1 种基金the National Science Fund for Distinguished Young Scholars ( Grant No. 50925932)the"Qinglan Project" of Jiangsu Province and Outstanding Doctoral Dissertation Incubation Program of Hohai University(Grant No.2010B18814)
文摘The eftects of various types of waves on vertical plane turbulent jets are studied numerically in the paper. A ttn＇ee- dimensional numerical model in a-coordinate is developed to study these problems by use of large eddy simulation method. Turbulence is modeled by a dynamic coherent eddy model. Numerical results including the distribution of veloci- ty, the decay law of the mean velocity along axis, the turbulent Reynolds stresses and the volume flux per unit width without wave, in the first-order Stokes waves, in the second-order Stokes waves, in the fifth-order Stokes waves, in the solitary waves and in random waves are compared and analyzed. A focus on coherent structures, probability density func- tions and correlation functions of jets is also investigated. The numerical results are of great theoretical importance for un- derstanding jet turbulent behaviors in different types of waves.
基金supported by the National Natural Science Foundation of China (50679023, 50879019)Ph.D. Programs Foundation of Ministry of Education of China (20070294012)+2 种基金the National Science Fund for Distinguished Young Scholars (50925932)Outstanding Doctoral Dissertation Incubation Program of Hohai University (2010B18814)Qing Lan Project of Jiangsu Province, and 333 High-Level Talent Training Program of Jiangsu Province (2017-B08038)
文摘The effect of random waves on vertical plane turbulent jets is studied numerically and the mechanism behind the interaction of the jet and waves is analyzed. The large eddy simulation method is used and the σ-coordinate system is adopted. Turbulence is modeled by a dynamic coherent eddy model. The σ-coordinate transformation is introduced to map the irregular physical domain with a wavy free surface and an uneven bottom onto a regular computational domain. The fractional step method is used to solve the filtered Navier–Stokes equations. Results presented include the distribution of velocity, the decay law of the mean velocity along the jet axis, self-similar characteristics and volume flux per unit width. In particular, the role of coherent structures on the momentum transfer along the jet centerline and the jet instantaneous characteristics in JONSWAP waves are a special focus of this research. The numerical results obtained are of great theoretical importance in understanding the behavior of turbulent jets in random wave environments.
基金Project(2011ZX07303-002) supported by National Water Pollution Control and Management Technology Major Projects,China
文摘The ability of a wet swale,constructed in an area of poor soil permeability,to manage runoff from a roadway was monitored through 27 storm events over a period of 8 months.During the monitoring period,the wet swale reduced the total runoff volume by 50.4%through exfiltration and evapotranspiration.The wet swale significantly decreased the influent pollutant concentrations,and the effluent mean concentrations of total suspended solids,total phosphorus,chemical oxygen demand,ammonium,oxidized nitrogen,and total nitrogen in the effluent were 31 mg/L,0.10 mg/L,29 mg/L,0.52 mg/L,0.35 mg/L and1.28 mg/L,respectively.Pollutant loads were also substantially reduced from 70%to 85%.Plant uptake played an important role in nutrient removal in the wet swale.Approximately half of the nitrogen(53.8%)and phosphorus(51.5%)that entered the wet swale was incorporated in above-ground plants.It is shown that wet swales are useful for managing runoff from roads in areas of poor soil permeability.
