DTL facilitates the Fanconi anemia pathway for ultraviolet-induced DNA repair in retinal pigment epithelial cells

The excessive energy of light,especially the invisible rays with lower wavelength,is basically absorbed by retinal pigment epithelium(RPE)and usually causes DNA damage.The molecular mechanism behind DNA damage repair response to this frequent stress in RPE is not clearly understood.In this study,we determined that the Fanconi anemia(FA)pathway was activated in human RPE ARPE-19 cells after ultraviolet(UV)B and C treatment.Moreover,immunoprecipitation(IP)of FANCD2 indicated that denticleless E3 ubiquitin protein ligase homolog(DTL)closely interacted with FANCD2.Knockdown of DTL weakened the activity of the FA pathway in ARPE-19 cells responding to UV treatment.Finally,the DTL promoter was incubated with a biotin-labeled probe and pulled down by streptavidin beads followed by the genomic DNA sonication.p53 was indicated by mass spectrum and further determined by chromatin IP assay.Taken together,our results demonstrated that DTL regulated by p53 could activate the FA pathway for UV-induced DNA damage repair in retinal pigment epithelial cells.

Health Management of Patients with Type II Diabetes and its Influence on Chronic Complication

Objectives:To explore the application of health management in patients with type II diabetes and to analyze and compare the incidence of chronic complications.Methods:the relevant research work was carried out in our hospital.During September 2018 to September 2019,100 patients with type II diabetes were randomly divided into two groups:one group was given routine nursing intervention,the other group was given health management,and they were named control group and experimental group respectively.Each group had 50 patients,the influence of different nursing methods on the incidence of chronic complications in patients was explored.Results:In the one-year followup results,the corresponding complications of patients mainly include cardiovascular disease,kidney disease,cerebrovascular disease,fundus disease and peripheral neuropathy.The incidence of complications in the experimental group is 10.00%,while that in the control group is 32.00%.in comparison,the incidence in the experimental group is lower,and the data difference between the two group is small(P<0.05),which exists significance.Conclusion:The application of health management method in type II diabetes has significant effect,which can reduce the incidence of chronic complications,improve the quality of life of patients,and has positive significance for clinical development.