A 4-mo history of both epigastralgia and back pain was presented in a 39-year-old male. Computed tomography showed right lung nodule and abdominal mass attached to the gastric wall, measuring approximately 30 mm and 7...A 4-mo history of both epigastralgia and back pain was presented in a 39-year-old male. Computed tomography showed right lung nodule and abdominal mass attached to the gastric wall, measuring approximately 30 mm and 70 mm in diameter. Since biopsy samples from the lung and abdomen revealed poorly differentiated adenocarcinoma and malignant tumor, clinicians first interpreted the abdominal mass as metastatic carcinoma, and a right lower lobectomy with following resection of the mass was performed. Gross examination of both lesions displayed gray-whitish to yellow-whitish cut surfaces with hemorrhagic and necrotic foci, and the mass attached to the serosa of the lesser curvature on the gastric body. On microscopic examination, the lung tumor was composed of a proliferation of highly atypical epithelial cells having abundant eosinophilic cytoplasm, predominantly arranged in an acinar or solid growth pattern with vessel permeation, while the abdominal tumor consisted of sheets or nests with markedly atypical epithelioid cells having pleomorphic nuclei and abundant eosinophilic to clear cytoplasm focally in a radial perivascular or infiltrative growth pattern. Immunohistochemically, the latter cells were positive for HMB45 or α-smooth muscle actin, but the former ones not. Therefore, we finally made a diagnosis of malignant perivascular epithelioid cell tumor(PEComa) arising in the gastric serosa, combined with primary lung adenocarcinoma. Furthermore, small papillary car-cinoma of the thyroid gland was identified. The current case describes the coincidence of malignant PEComa with other carcinomas, posing a challenge in distinction from metastatic tumor disease.展开更多
Precise and reliable presentation of odorants to animals is crucial for olfactory studies.Although odor stimulation systems in anesthetized or awake,head-fixed animals are well established,temporally precise odor prese...Precise and reliable presentation of odorants to animals is crucial for olfactory studies.Although odor stimulation systems in anesthetized or awake,head-fixed animals are well established,temporally precise odor presentation in awake,freely moving animals remains a challenge.Here,we describe a new odor stimulation system which presents odors directly to the nostrils of freely moving mice.The system comprises 3 modules:an odor-delivery module,an odor-generation module,and a control module.The new system is precise and temporally reliable,and odor stimulation can be triggered by specific sniffing phases or other events.Moreover,the system can be combined with neural recordings,such as electrophysiology,and olfactory behavioral tests to investigate how neurons in the brain represent odor information during individual olfactory behaviors.This innovative odor stimulation system may replace traditional stimulation systems:It will enable precise odor presentation in a wide range of olfactory studies in freely moving animals.展开更多
文摘A 4-mo history of both epigastralgia and back pain was presented in a 39-year-old male. Computed tomography showed right lung nodule and abdominal mass attached to the gastric wall, measuring approximately 30 mm and 70 mm in diameter. Since biopsy samples from the lung and abdomen revealed poorly differentiated adenocarcinoma and malignant tumor, clinicians first interpreted the abdominal mass as metastatic carcinoma, and a right lower lobectomy with following resection of the mass was performed. Gross examination of both lesions displayed gray-whitish to yellow-whitish cut surfaces with hemorrhagic and necrotic foci, and the mass attached to the serosa of the lesser curvature on the gastric body. On microscopic examination, the lung tumor was composed of a proliferation of highly atypical epithelial cells having abundant eosinophilic cytoplasm, predominantly arranged in an acinar or solid growth pattern with vessel permeation, while the abdominal tumor consisted of sheets or nests with markedly atypical epithelioid cells having pleomorphic nuclei and abundant eosinophilic to clear cytoplasm focally in a radial perivascular or infiltrative growth pattern. Immunohistochemically, the latter cells were positive for HMB45 or α-smooth muscle actin, but the former ones not. Therefore, we finally made a diagnosis of malignant perivascular epithelioid cell tumor(PEComa) arising in the gastric serosa, combined with primary lung adenocarcinoma. Furthermore, small papillary car-cinoma of the thyroid gland was identified. The current case describes the coincidence of malignant PEComa with other carcinomas, posing a challenge in distinction from metastatic tumor disease.
基金supported by the National Natural Science Foundation of China(NSFC,31872771 and 32070995 to Anan Li,31872770 to Yufeng Shao)the Jiangsu Province Innovative and Entrepreneurial Team Program.
文摘Precise and reliable presentation of odorants to animals is crucial for olfactory studies.Although odor stimulation systems in anesthetized or awake,head-fixed animals are well established,temporally precise odor presentation in awake,freely moving animals remains a challenge.Here,we describe a new odor stimulation system which presents odors directly to the nostrils of freely moving mice.The system comprises 3 modules:an odor-delivery module,an odor-generation module,and a control module.The new system is precise and temporally reliable,and odor stimulation can be triggered by specific sniffing phases or other events.Moreover,the system can be combined with neural recordings,such as electrophysiology,and olfactory behavioral tests to investigate how neurons in the brain represent odor information during individual olfactory behaviors.This innovative odor stimulation system may replace traditional stimulation systems:It will enable precise odor presentation in a wide range of olfactory studies in freely moving animals.
