Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram...Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram（4-amino-3,5,6-trichloropicolinic acid） at different concentrations of carbohydrate under the same conditions with presence or absence of luminosity.In our experiment,secondary explants were kept in MS medium containing 8.0 mg·L-1 of Picloram for the callus formation.Calluses were transferred in MS medium supplemented with sucrose,fructose and glucose（control,2%,4% and 6%）.Results show that Picloram induced the callogenesis in leaf sheath.The secondary embryogenesis was formed in yellow-globular callus.The sucrose as carbohydrate source in the absence of light was more efficient to induce rhizogenesis.Glucose was more efficiency in the presence of light.Callogenic induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.展开更多
Eukaryotic cells contain semi-autonomous organelles that have lost most of their coding capacity for essential proteins to the nucleus.Therefore,their functioning depends on the cytosolic translation products of nucle...Eukaryotic cells contain semi-autonomous organelles that have lost most of their coding capacity for essential proteins to the nucleus.Therefore,their functioning depends on the cytosolic translation products of nuclear-encoded genes that must be imported by a specialized protein import machinery.Chloroplasts import between 2500 and 3500 proteins for their functioning,demonstrating that the bulk of the chloroplast proteome is shuttied through the cytosol as precursor proteins.Precursor proteins usually contain an N-terminal transit peptide that is cleaved off by a signa/processing peptidase(SPP)and degraded by the chloroplast protease network upon import.In the cytosol,precursors can be co-and post-translationally modified,interact with a set of chaperones,co-chaperones,14-3-3 proteins,and/or E3 ligases before they are either imported or degraded by the cytosolic proteasome complex.It is now clear that precursors are not only a transient transport form of functional proteins but that their cytosolic transition is of regulatory importance.展开更多
基金supported by FAPESP (So Paulo Research Foundation, Brazil)CAPES (Coordination for the Improvement of Higher Level, Brazil)
文摘Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram（4-amino-3,5,6-trichloropicolinic acid） at different concentrations of carbohydrate under the same conditions with presence or absence of luminosity.In our experiment,secondary explants were kept in MS medium containing 8.0 mg·L-1 of Picloram for the callus formation.Calluses were transferred in MS medium supplemented with sucrose,fructose and glucose（control,2%,4% and 6%）.Results show that Picloram induced the callogenesis in leaf sheath.The secondary embryogenesis was formed in yellow-globular callus.The sucrose as carbohydrate source in the absence of light was more efficient to induce rhizogenesis.Glucose was more efficiency in the presence of light.Callogenic induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.
文摘Eukaryotic cells contain semi-autonomous organelles that have lost most of their coding capacity for essential proteins to the nucleus.Therefore,their functioning depends on the cytosolic translation products of nuclear-encoded genes that must be imported by a specialized protein import machinery.Chloroplasts import between 2500 and 3500 proteins for their functioning,demonstrating that the bulk of the chloroplast proteome is shuttied through the cytosol as precursor proteins.Precursor proteins usually contain an N-terminal transit peptide that is cleaved off by a signa/processing peptidase(SPP)and degraded by the chloroplast protease network upon import.In the cytosol,precursors can be co-and post-translationally modified,interact with a set of chaperones,co-chaperones,14-3-3 proteins,and/or E3 ligases before they are either imported or degraded by the cytosolic proteasome complex.It is now clear that precursors are not only a transient transport form of functional proteins but that their cytosolic transition is of regulatory importance.
