OBJECTIVE： To investigate the anti-inflammatory effect of pretreatment with quercetin on macro- phages after Candida albicans infection. METHODS： RAW 264.7 macrophages were used as a target cell line. Cell viability...OBJECTIVE： To investigate the anti-inflammatory effect of pretreatment with quercetin on macro- phages after Candida albicans infection. METHODS： RAW 264.7 macrophages were used as a target cell line. Cell viability after treatment with quercetin at different time points was detected by Carboxyfluorescein diacetate, succinimidyl ester. Phagocytic function of macrophages was deter- mined by a fluorometric assay. Cytokine tumor ne- crosis factor a （TNF-a） production was measured by enzyme-linked immunosorbent assay. F-actin cy- toskeleton of L929 cells was stained by Alexa Fluor 488-phalloidin. RESULTS： Pretreatment with quercetin decreasedcell viability only at the highest concentration of 37 μg/mL 2, 24, and 48 h after the treatment. The phagocytic efficiency of macrophages pretreated with quercetin was significantly decreased in a time- and dose-dependent manner. F-actin label- ing showed that the actin cytoskeleton of the cells started to break down 2 h after treatment. More- over, it notably inhibited cytokine TNF-a produc- tion after Candida albicans infection. CONCLUSION： Pretreatment with quercetin in- duced an anti-inflammatory effect against Candida albicans infection in macrophages.展开更多
基金Supported by Grants from Scientific Research Foundation of the Higher Education Institutions of Jiangsu Province,China(No.13KJB310022)Administration of Traditional Chinese Medicine of Jiangsu Province(No.LZ13248)China Postdoctoral Science Foundation(No.2013M541741)
文摘OBJECTIVE： To investigate the anti-inflammatory effect of pretreatment with quercetin on macro- phages after Candida albicans infection. METHODS： RAW 264.7 macrophages were used as a target cell line. Cell viability after treatment with quercetin at different time points was detected by Carboxyfluorescein diacetate, succinimidyl ester. Phagocytic function of macrophages was deter- mined by a fluorometric assay. Cytokine tumor ne- crosis factor a （TNF-a） production was measured by enzyme-linked immunosorbent assay. F-actin cy- toskeleton of L929 cells was stained by Alexa Fluor 488-phalloidin. RESULTS： Pretreatment with quercetin decreasedcell viability only at the highest concentration of 37 μg/mL 2, 24, and 48 h after the treatment. The phagocytic efficiency of macrophages pretreated with quercetin was significantly decreased in a time- and dose-dependent manner. F-actin label- ing showed that the actin cytoskeleton of the cells started to break down 2 h after treatment. More- over, it notably inhibited cytokine TNF-a produc- tion after Candida albicans infection. CONCLUSION： Pretreatment with quercetin in- duced an anti-inflammatory effect against Candida albicans infection in macrophages.
