There is increasing evidence that,in addition to their presence,the propensity of circulating tumour cells to form multi-cellular clusters bears significant information about both cellular resistance to chemotherapy a...There is increasing evidence that,in addition to their presence,the propensity of circulating tumour cells to form multi-cellular clusters bears significant information about both cellular resistance to chemotherapy and overall prognosis.We present a novel two-stage,stopped-flow,continuous centrifugal sedimentation strategy to measure the size distributions of events(defined here as cells or clusters thereof)in a blood sample.After off-chip removal of red blood cells,healthy white blood cells are sequestered by negative-immunocapture.The purified events are then resolved along a radially inclined rail featuring a series of gaps with increasing width,each connected to a designated outer collection bin.The isolation of candidate events independent of targetspecific epitopes is successfully demonstrated for HL60(EpCAM positive)and sk-mel28(EpCAM negative)cells using identical protocols and reagents.The propensity to form clusters was quantified for a number of cell lines,showing a negligible,moderate or elevated tendency towards cluster formation.We show that the occupancy distribution of the collection bins closely correlates with the range of cluster sizes intrinsic to the specific cell line.展开更多
For the first time,we harness the unique phase-selectivity of chip-integrated graphene oxide(GO)membranes to significantly enhance flow control on centrifugal microfluidic platforms.In this paper,we present novel proc...For the first time,we harness the unique phase-selectivity of chip-integrated graphene oxide(GO)membranes to significantly enhance flow control on centrifugal microfluidic platforms.In this paper,we present novel processes for the assembly of these GO membranes into polymeric microfluidic systems and demonstrate that multilayer GO membranes allow the passage of water while blocking pressurized air and organic solutions.展开更多
基金This work was supported in part by Enterprise Ireland(Grant No.CF 20111317)Science Foundation Ireland(Grant No.10/CE/B1821).
文摘There is increasing evidence that,in addition to their presence,the propensity of circulating tumour cells to form multi-cellular clusters bears significant information about both cellular resistance to chemotherapy and overall prognosis.We present a novel two-stage,stopped-flow,continuous centrifugal sedimentation strategy to measure the size distributions of events(defined here as cells or clusters thereof)in a blood sample.After off-chip removal of red blood cells,healthy white blood cells are sequestered by negative-immunocapture.The purified events are then resolved along a radially inclined rail featuring a series of gaps with increasing width,each connected to a designated outer collection bin.The isolation of candidate events independent of targetspecific epitopes is successfully demonstrated for HL60(EpCAM positive)and sk-mel28(EpCAM negative)cells using identical protocols and reagents.The propensity to form clusters was quantified for a number of cell lines,showing a negligible,moderate or elevated tendency towards cluster formation.We show that the occupancy distribution of the collection bins closely correlates with the range of cluster sizes intrinsic to the specific cell line.
基金This work is partially funded under the Programme for Research in Third Level Institutions(PRTLI)Cycle 5The PRTLI is co-funded through the European Regional Development Fund(ERDF)part of the European Union Structural Funds Programme 2007–2013.
文摘For the first time,we harness the unique phase-selectivity of chip-integrated graphene oxide(GO)membranes to significantly enhance flow control on centrifugal microfluidic platforms.In this paper,we present novel processes for the assembly of these GO membranes into polymeric microfluidic systems and demonstrate that multilayer GO membranes allow the passage of water while blocking pressurized air and organic solutions.
