Fluorogenic biosensors are essential tools widely used in biomedicine,chemical biology,environmental protection and food safety.Fluorescence resonance energy transfer(FRET)is a crucial technique for developing fluorog...Fluorogenic biosensors are essential tools widely used in biomedicine,chemical biology,environmental protection and food safety.Fluorescence resonance energy transfer(FRET)is a crucial technique for developing fluorogenic biosensors that provide mechanistic insight into bioprocesses through time-spatial bioimaging in living cells and organisms.Although extensive FRET-based sensors have been developed for detecting or imaging analytes of interest over the past decade,few comprehensive reviews have summarized the recent studies from the fundamental chemical angle about the design and application.In this work,the recent advance in the discovery of FRET biosensors using donor-acceptor dye combinations is described and they are classified based on different types of analytes,such as mall molecules,proteins,enzymes,nucleic acids and metal ions.This review provides molecular-level inspiration for the design of FRET-based biosensors,aiding in their application in biosensing and bioimaging.展开更多
基金National Natural Science Foundation of China,Grant/Award Numbers:U20A2038,32372584,22174121,22211530067,T22507101802022 Natural Science Foundation of Guangdong Provincial Basic and Applied Basic Research Fund(Guangdong Hybribio),Guangdong Peral River Talent Program,Grant/Award Number:2021CX02Y066+1 种基金Shenzhen Bay Open Laboratory Fund 2021 by Shenzhen Bay Laboratory,CUHK(SZ)-Boyalife Joint Laboratory Fund,CUHK(SZ)-The Second Affiliated Hospital Joint Fund,Grant/Award Number:YXLH2205University Development Fund,Grant/Award Number:UDF01002012。
文摘Fluorogenic biosensors are essential tools widely used in biomedicine,chemical biology,environmental protection and food safety.Fluorescence resonance energy transfer(FRET)is a crucial technique for developing fluorogenic biosensors that provide mechanistic insight into bioprocesses through time-spatial bioimaging in living cells and organisms.Although extensive FRET-based sensors have been developed for detecting or imaging analytes of interest over the past decade,few comprehensive reviews have summarized the recent studies from the fundamental chemical angle about the design and application.In this work,the recent advance in the discovery of FRET biosensors using donor-acceptor dye combinations is described and they are classified based on different types of analytes,such as mall molecules,proteins,enzymes,nucleic acids and metal ions.This review provides molecular-level inspiration for the design of FRET-based biosensors,aiding in their application in biosensing and bioimaging.
