IGF2BPs: a family as diagnostic and prognostic biomarkers in digestive system tumors

The highest morbidity and mortality in the world are attributed to digestive system tumors,such as stomach cancer,liver cancer,and pancreatic cancer.Exploring potential biomarkers is a crucial direction of tumor research.We use bioinformatics methods to explore potential biomarkers of the digestive system.Mining and analyzing data from Gene Expression Profiling Interactive Analysis(GEPIA),Kaplan-Meier,cBioPortal,and Metabolic gEne RApid Visualizer(MERAV)to explore the correlation between IGF2BP(insulin-like growth factor-2 mRNA-binding protein)family expression and immune infiltration in digestive system tumors,and further probe the prognostic value of IGF2BP family in digestive system tumors.Esophageal cancer tissues showed a significantly higher expression of IGF2BP2 than normal tissues,while IGF2BP3 was notably more expressed in esophageal cancer,pancreatic cancer,and stomach cancer.In the prognosis evaluation,the IGF2BP1 gene in patients with liver cancer and the IGF2BP2 and IGF2BP3 genes in patients with stomach cancer and liver cancer of the low gene expression level groups were better.Multivariate COX regression analysis further suggested that tumor stage,CD8 positive T cells,macrophages,dendritic cell infiltration,and IGF2BP3 expression were independent risk factors affecting the prognosis of patients with stem cell liver cancer.The IGF2BP family may be a potential marker for immunotherapy and the prognosis of digestive system tumors.

Sirt5 desuccinylates Cdc42 to mediate osteoclastogenesis and bone remodeling in mice

Post-translational modifications(PTMs)play a critical role in bone remodeling,with phosphorylation and acetylation being particularly well characterized.Recently,succinylation,a relatively uncommon PTM on lysine,has received considerable research attention for its influence on several physiological and pathological processes and conditions.1 Several substrates involved in mitochondrial pathways have been validated as substrates for the desuccinylase sirtuin 5(Sirt5),a key regulator of succinylation.2 Bone is one of the most metabolically active organs,but the role of succinylation during bone remodeling is not well characterized.

Coordination of EZH2 and SOX2 specifies human neural fate decision

Polycomb repressive complexes(PRCs)are essential in mouse gastrulation and specify neural ectoderm in human embryonic stem cells(hESCs),but the underlying molecular basis remains unclear.Here in this study,by employing an array of different approaches,such as gene knock-out,RNA-seq,ChIP-seq,et al.,we uncover that EZH2,an important PRC factor,specifies the normal neural fate decision through repressing the competing meso/endoderm program.EZH2^(−/−)hESCs show an aberrant re-activation of meso/endoderm genes during neural induction.At the molecular level,EZH2 represses meso/endoderm genes while SOX2 activates the neural genes to coordinately specify the normal neural fate.Moreover,EZH2 also supports the proliferation of human neural progenitor cells(NPCs)through repressing the aberrant expression of meso/endoderm program during culture.Together,our findings uncover the coordination of epigenetic regulators such as EZH2 and lineage factors like SOX2 in normal neural fate decision.