Rheumatoid arthritis(RA),which is marked by inflammatory synovitis,is a common,chronic autoim-mune-disease,whose pathogenesis is complex and still unclear.In order to explore the effects of heat and hyposmotic stimuli...Rheumatoid arthritis(RA),which is marked by inflammatory synovitis,is a common,chronic autoim-mune-disease,whose pathogenesis is complex and still unclear.In order to explore the effects of heat and hyposmotic stimuli on synoviocytes in rheumatoid arthritis,the changes of [Ca2+]i induced by heat,hyposmotic and 4α-PDD stimuli were observed in synoviocytes.[Ca2+]i elevation induced by heat ≥ 28℃,hyposmotic and 4α-PDD stimuli is found to be positively relative to increasing temperature,decreasing osmolality and rising concentration of 4α-PDD.Results show that there is reciprocity among these stimuli and desensitization,and that [Ca2+]i elevation depends on Ca2+ influx,but not necessarily links to Ca2+ release from intracellular stores and voltage-dependent Ca2+ channel in synoviocytes.The above characteristics of Ca2+ influx are similar to those of TRPV4.A probable mechanism has been suggested that heat and hyposmotic stimulation might increase the level of [Ca2+]i by activating the TRPV4-like channel and Ca2+ influx in the synoviocytes.展开更多
基金Supported by a foundation of the City of Tianjin,China (Grand No. 983113411)
文摘Rheumatoid arthritis(RA),which is marked by inflammatory synovitis,is a common,chronic autoim-mune-disease,whose pathogenesis is complex and still unclear.In order to explore the effects of heat and hyposmotic stimuli on synoviocytes in rheumatoid arthritis,the changes of [Ca2+]i induced by heat,hyposmotic and 4α-PDD stimuli were observed in synoviocytes.[Ca2+]i elevation induced by heat ≥ 28℃,hyposmotic and 4α-PDD stimuli is found to be positively relative to increasing temperature,decreasing osmolality and rising concentration of 4α-PDD.Results show that there is reciprocity among these stimuli and desensitization,and that [Ca2+]i elevation depends on Ca2+ influx,but not necessarily links to Ca2+ release from intracellular stores and voltage-dependent Ca2+ channel in synoviocytes.The above characteristics of Ca2+ influx are similar to those of TRPV4.A probable mechanism has been suggested that heat and hyposmotic stimulation might increase the level of [Ca2+]i by activating the TRPV4-like channel and Ca2+ influx in the synoviocytes.
