This study aims to understand the relationship between capabilities of Escherichia coli strains to form biofilm and serotype groups expressed on cell surface. Sixteen strains of E. coli were originally isolated from d...This study aims to understand the relationship between capabilities of Escherichia coli strains to form biofilm and serotype groups expressed on cell surface. Sixteen strains of E. coli were originally isolated from different food processing lines in different Moroccan cities. Strains serotyped based on their O (somatic), H (flagellar), and K (capsular) surface antigen profiles using different antiserums. Biofilm assays carried out in 96-well microtiter dishes using the method of O’Toole et al. Our results show that no clear relation observed between origin and serotype groups. In the other hand, we observed that not all studied strains were able to form biofilm. Furthermore, combination of antigens H40 and K11 appears to be involved in biofilm formation. In fact, the H antigen seems to be implicated in the placement of the bacterial cells near the surface and the K antigen may play a role in physicochemical interactions between bacteria and inert surface.展开更多
文摘This study aims to understand the relationship between capabilities of Escherichia coli strains to form biofilm and serotype groups expressed on cell surface. Sixteen strains of E. coli were originally isolated from different food processing lines in different Moroccan cities. Strains serotyped based on their O (somatic), H (flagellar), and K (capsular) surface antigen profiles using different antiserums. Biofilm assays carried out in 96-well microtiter dishes using the method of O’Toole et al. Our results show that no clear relation observed between origin and serotype groups. In the other hand, we observed that not all studied strains were able to form biofilm. Furthermore, combination of antigens H40 and K11 appears to be involved in biofilm formation. In fact, the H antigen seems to be implicated in the placement of the bacterial cells near the surface and the K antigen may play a role in physicochemical interactions between bacteria and inert surface.
