Sea cucumber saponins have attracted more attention in recent years due to biological activities.It is a popular practice to soak sea cucumber in Baijiu at home and being applied to industrial manufacturing in China.H...Sea cucumber saponins have attracted more attention in recent years due to biological activities.It is a popular practice to soak sea cucumber in Baijiu at home and being applied to industrial manufacturing in China.However,knowledge of the effect of alcohol on the absorption and metabolism of sea cucumber saponins is limited.The effects of alcohol on digestion,absorption and metabolism of sea cucumber saponins in BALB/c mice were investigated after gavage and tail intravenous injection.The results showed that the content of saponins in serum and liver was significantly higher under the influence of alcohol than that in the control group after oral administration.Alcohol promoted the absorption of sea cucumber saponins prototype as well as inhibited the process of saponins being transformed into deglycositic metabolites in the small intestine.Moreover,sea cucumber saponins remained in circulation for a long time and alcohol slowed down the clearance of sea cucumber saponins under the influence of alcohol after intravenous injection.This confirmed the feasibility of marinating sea cucumber in Baijiu to improve the efficacy of saponins and provides an important theoretical basis for the utilization of sea cucumber and the development of sea cucumber liquor.展开更多
Trimethylamine N-oxide(TMAO)is a risk factor of various chronic diseases,which was produced by metabolism from precursors to trimethylamine(TMA)in gut and the oxidation from TMA in liver.The TMA generation was influen...Trimethylamine N-oxide(TMAO)is a risk factor of various chronic diseases,which was produced by metabolism from precursors to trimethylamine(TMA)in gut and the oxidation from TMA in liver.The TMA generation was influenced by diet,mainly due to the rich TMAO precursors in diet.However,it was still unclear that the effects of different proportion and source of macronutrients in different dietary pattern on the production process of TMAO.Here,the generation of TMA from precursors and TMAO from TMA was determined after single oral choline chloride and intraperitoneal injection TMA,respectively,in mice fed with carbohydrates,proteins and fats in different proportion and sources.The results suggested that the generation of TMAO was increased by low non-meat protein and high fat via enhancing the production of TMAO from TMA,and decreased by plant protein and refined sugar via reducing TMA production from precursors in gut and TMAO transformation from TMA in liver.The high fat and high sugar diets accelerating the development of atherosclerosis did not increase the production of TMAO,the risk factor for atherosclerosis,which indicated that the dietary compositions rather than the elevated TMAO level might be a more key risk factor for atherosclerosis.展开更多
Sensitive monitoring of the target products during the biosynthesis process is crucial,and facile analytical approaches are urgently needed.Herein,phosphatidylserine(PS)was chosen as the model target,a colorimetric ap...Sensitive monitoring of the target products during the biosynthesis process is crucial,and facile analytical approaches are urgently needed.Herein,phosphatidylserine(PS)was chosen as the model target,a colorimetric aptasensor was developed for the rapid quantitation in biosynthesis samples.A chimeric aptamer was constructed with two homogeneous original PS aptamers.Specific recognition between the chimeric aptamer and PS results in the desorption of aptamer from the surface of the AuNPs nanozyme,and the peroxidase-like enzymatic activity of the AuNPs nanozyme was weakened in a relationship with the different concentrations.The developed aptasensor performed well when applied for analyzing PS in biosynthesis samples.The aptasensor offers good sensitivity and selectivity,under optimal conditions,achieving monitoring and quantitation of PS in the range of 2.5-80.0μmol/L,with a limit of detection at 536.2 nmol/L.Moreover,the aptasensor provides good accuracy,with comparison rates of 98.17%-106.40%,when compared with the HPLC-ELSD.This study provides a good reference for monitoring other biosynthesized products and promoting the development of aptamers and aptasensors in real-world applications.展开更多
Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived end...Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum HDN11-186.Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass spectra.The absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)spectrum.UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured fungi.Compound 1 showed obvious antibacterial activities against Pro-teus sp.MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).展开更多
Renewable electrocatalytic upgrading of biomass feedstocks into valuable chemicals is one of the promising strategies to relieve the pressure of traditional energy-based systems.Through electrocatalytic carbon–carbon...Renewable electrocatalytic upgrading of biomass feedstocks into valuable chemicals is one of the promising strategies to relieve the pressure of traditional energy-based systems.Through electrocatalytic carbon–carbon bond cleavage of high selectivity,various functionalized molecules,such as organic acids,amides,esters,and nitriles,have great potential to be accessed from biomass.However,it has merely received finite concerns and interests in the biorefinery.This review first showcases the research progress on the electrocatalytic conversion of lipid/sugar-and lignin-derived molecules(e.g.,glycerol,mesoerythritol,xylose,glucose,1-phenylethanol,and cyclohexanol)into organic acids via specific carbon–carbon bond scission processes,with focus on disclosing reaction mechanisms,recognizing actual active species,and collecting feasible modification strategies.For the guidance of further extensive studies on biomass valorization,organic transformations via a variety of reactions,including decarboxylation,ring-opening,rearrangement,reductive hydrogenation,and carboxylation,are also disclosed for the construction of similar carbon skeletons/scaffolds.The remaining challenges,prospective applications,and future objectives in terms of biomass conversion are also proposed.This review is expected to provide references to develop renewed electrocatalytic carbon–carbon bond cleavage transformation paths/strategies for biomass upgrading.展开更多
The aim of this research work was to evaluate the potential of using renewable natural feedstock,i.e.,Jatropha curcas oil(JCO)for the synthesis of non-isocyanate polyurethane(NIPU)resin for wood composite applications...The aim of this research work was to evaluate the potential of using renewable natural feedstock,i.e.,Jatropha curcas oil(JCO)for the synthesis of non-isocyanate polyurethane(NIPU)resin for wood composite applications.Commercial polyurethane(PU)is synthesized through a polycondensation reaction between isocyanate and poly-ol.However,utilizing toxic and unsustainable isocyanates for obtaining PU could contribute to negative impacts on the environment and human health.Therefore,the development of PU from eco-friendly and sustainable resources without the isocyanate route is required.In this work,tetra-n-butyl ammonium bromide was used as the activator to open the epoxy ring with 3-Aminopropyltriethoxisylane as a catalyst to yield urethane of JCO(UJCO).The UJCO were characterized by Fourier Transform Infra-Red spectroscopy(FTIR)and their oxirane,and hydroxyl values were measured.The result showed that a decrease in oxirane value was found while the hydroxyl value was increased during the time,confirming that the urethane group was formed.The presence of functional groups in FTIR spectra at wave numbers 1732.08,1562.34,and 3348.42 cm^(−1) indicates the functional groups of C=O(urethane carbonyl),–NH,and–OH,respectively confirmed this finding.The potential applications of NIPU in the wood composite were also outlined.展开更多
In this study,we proposed a reliable and sustainable technique for the clean utilization of shrimp wastes,which can yield a solid inoculant of Bacillus subtilis OKF04 containing micronutrients at low cost without the ...In this study,we proposed a reliable and sustainable technique for the clean utilization of shrimp wastes,which can yield a solid inoculant of Bacillus subtilis OKF04 containing micronutrients at low cost without the risk of contamination.Study of the culture conditions revealed that the head of shrimp Litopenaus vannamei and the wheat bran acted as suitable substrates for the growth of B.subtilis OKF04.With 60%initial moisture content,30℃culture temperature,and 5%inoculation amount,followed by 48 hours of fermentation and 0.5%soluble starch added during the drying process(50℃for 6h),a solid B.subtilis OKF04 inoculant with a spore amount of 2.4×10^(10)CFU g^(-1)and a high amino acid content was obtained.The solid B.subtilis OKF04 inoculant was applied to cultivate pakchoi under pot experiment.As the result,of adding to,the size of stems and leaves,nutritional composition,and physiological activity of pakchoi were significantly(P<0.05)enhanced by solid B.subtilis OKF04 inoculant.B.subtilis OKF04 also significantly(P<0.05)increased the soil’s nutrient content and improved its microbial composition.Furthermore,pakchoi cultivated with a low dose of solid B.subtilis OKF04 inoculant(0.05 g kg^(-1)soil)resulted in the best results.This study provides a new method for the preparation of microbial inoculants with solid waste shrimp heads.展开更多
Adsorption dynamics of ethane in two granular fixed beds and structured fixed beds with microfibrous composites was studied.5A zeolite membrane 5A/PSSF(paper-like sintered stainless steel fiber)and microfibrous entrap...Adsorption dynamics of ethane in two granular fixed beds and structured fixed beds with microfibrous composites was studied.5A zeolite membrane 5A/PSSF(paper-like sintered stainless steel fiber)and microfibrous entrapped activated carbon(MEAC)composites were prepared by wet layup papermaking/sintering technique and in-situ hydrothermal method.Microfibrous composites were characterized by X-ray diffraction,scanning electron microscopy and N2 adsorption/desorption.Structured fixed beds were designed by filling granular adsorbents(5A zeolite or activated carbon)and microfibrous composites at the inlet and outlet of the beds,respectively.Effects of flow rate,bed height and structure on the breakthrough curves were investigated.The length of unused bed(LUB)was determined,and Yoon–Nelson model was used to fit the breakthrough curves.The experimental results showed ethane was effectively adsorbed on the granular adsorbents and microfibrous composites.Both composites could decrease the LUB values and enhance bed utilization.All breakthrough curves fitted well to Yoon–Nelson model,with correlation coefficient exceeding 0.89.The adsorption rate of ethane could be improved in the structured fixed beds,which showed an enhanced mass transfer efficiency for ethane adsorption.LUB values of structured fixed beds with 5A/PSSF composites were larger,the bed utilization values were lower,and the adsorption rate constants were higher than those with MEAC composites under the same conditions.展开更多
Laminarinases reveal potential application in the field of food and biotechnology.In this research,a novel GH16 family laminarinase,designated as Lam16A_Wa,was cloned from the genome of marine bacterium Wenyingzhuangi...Laminarinases reveal potential application in the field of food and biotechnology.In this research,a novel GH16 family laminarinase,designated as Lam16A_Wa,was cloned from the genome of marine bacterium Wenyingzhuangia aestuarii OF219 and expressed in Escherichia coli.Lam16A_Wa demonstrates a relatively low optimal reaction temperature(35℃)and a cold-adapted feature.Its optimal pH value is 6.0 and is stable in a broad pH range from 3.0 to 11.0.A glycomics strategy was employed to investigate the hydrolytic pattern of Lam16A_Wa.The enzyme was confirmed as a random endo-acting glycoside hydrolase.Its minimum substrate was laminarin pentasaccharide,and the major final products are oligosaccharides,including disaccharide to pentasaccharide.The Lam16A_Wa provides a novel and well-defined tool for the molecular tailoring of laminarin.展开更多
Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regula...Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia coli.To enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)mutants.To test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for expression.The enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 promoter.In high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 promoter.Therefore,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.展开更多
The Janus kinases(JAKs)are a family of intracellular tyrosine kinases that play an essential role in many basic biological processes,such as apoptosis and inflammation.Thus any dysfunction of the proteins in this path...The Janus kinases(JAKs)are a family of intracellular tyrosine kinases that play an essential role in many basic biological processes,such as apoptosis and inflammation.Thus any dysfunction of the proteins in this pathway may lead to a variety of diseases,including cancer and diseases that affect the immune system,such as severe combined immune deficient(SCID).Marine biological resources have become an important source in new drug research and development due to their diversity,complexity and speciality.In this study,Marine alkaloid Neobacillamide A was isolated from the greedy and stubborn sponge symbiotic Bacillus atrophicus C89 in the South China Sea.Totally 24 novel marine alkaloid Neobacillamide A derivatives were designed and synthesized,which were evaluated for their inhibitory activity against JAK/STAT signaling pathway and their cytotoxicity to A549 cells.Compounds 13c,13o,14d,14g and 14h showed potent JAK/STAT inhibition capability(concentration of 25μmol L^(-1),all inhibitory potencies were above 60%),especially compound 14g exhibited superior JAK/STAT inhibition effect(89.70%inhibition).In addition,all these compounds with a concentration of 25μmol L^(-1)displayed weak or no cytotoxicity to A549 cells,which means that these Neobacillamide A derivatives with JAK/STAT inhibition capability may have potential anti-inflammatory function.展开更多
A sensitive,rapid,and robust ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was established for the first time to quantify agarotriose(A3)in rat plasma,tissues,urine,and feces...A sensitive,rapid,and robust ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was established for the first time to quantify agarotriose(A3)in rat plasma,tissues,urine,and feces.A3 and stachyose(internal standard)were separated by a BEH amide column at 65℃under the mobile phase of 10 mmol L^(-1)ammonium ace-tate-acetonitrile(42:58,v/v)with 350µLmin-1.The acquisition of transitions was carried out in multiple reaction monitoring(MRM)pattern operating with positive ionization at m/z 509.16>329.15 for A3 and m/z 689.15>527.11 for stachyose.The linearity ranges of A3 were 10 to 5000nmolL^(-1)for plasma,20 to 10000nmolL^(-1)for tissues,and 40 to 20000nmolL^(-1)for urine and feces.The accuracy and precision ranged from 90.9%to 111.6%and 0.7%to 10.1%,respectively.The stability was between 86.1%and 102.5%.The extraction recovery was consistent and reproducible.The matrix effect ranged from 1.5%to 11.4%.The pharmacokinetic,tissue dis-tribution,and excretion studies were successfully conducted with the validated method.Results showed that A3 could be absorbed by rats,and the absolute bioavailability was 6.7%.Furthermore,it was rapidly distributed in rat tissues and mainly eliminated via feces excretion(67.0%)after oral administration.For intravenous bolus,85.5%was recovered,and renal excretion was the primary path-way(77.6%)for cumulative recovery.展开更多
Chemical epigenetic manipulation was applied to the zoanthid-derived fungus Cochliobolus lunatus(TA26-46)with a histone deacetylation modifier(100μmol L^(−1) nicotinamide),resulting in the isolation of a new 14-membe...Chemical epigenetic manipulation was applied to the zoanthid-derived fungus Cochliobolus lunatus(TA26-46)with a histone deacetylation modifier(100μmol L^(−1) nicotinamide),resulting in the isolation of a new 14-membered resorcylic acid lactone named 7′(Z)-zeaenol(1),together with six known analogues(2−7)from the treated broth.The planar structure of 1 was determined by comprehensive NMR spectroscopy and HRESIMS data.The absolute configuration of 1 was elucidated by ECD spectrum,^(13)C NMR shift calculations,and on the basis of biogenetic considerations.Compound 5 exhibited cytotoxic activity against the human tumor cell lines A549,HCT-116,HT-29,Hela,MCF-7,and K562 with the IC_(50) values ranging from 2.54 to 7.44μmolL^(−1).展开更多
The marine biopharmaceutical industry(MBI)has been considered as an important part of the blue economy.The high-quality development of this industry depends on the high-level coordinated development of technological i...The marine biopharmaceutical industry(MBI)has been considered as an important part of the blue economy.The high-quality development of this industry depends on the high-level coordinated development of technological innovation system(TIS).In the present study,the coupling mechanism of industrial innovation input subsystem and innovation output subsystem was analyzed for the first time.On this basis,the development level and coupling coordination level of TIS in China’s MBI during 2008-2018 were empirically evaluated with the capacity coupling coordination model.Then,the obstacle factors were diagnosed and recognized with the obstacle model.The results showed that the innovation input index fluctuated at a low level in China’s MBI.The innovation output index has basically maintained a growth trend,whereas the quality of development was not high.Although the coupling coordination level of TIS showed a positive change as mild disordered→primary coordinated→well-coordinated,the development type of innovation system has changed from the lagging output of innovation into the lagging input of innovation.Insufficient input of innovation factors remained the main obstacle to the improvement of coordination level.Based on the above analysis,suggestions were put forward from the perspectives of policy and fund guarantees to improve the coupling coordination level in China’s MBI.展开更多
Sugar palm(Arenga pinnata)starch is considered an important renewable,biodegradable,and eco-friendly polymer,which is derived from agricultural by-products and residues,with great potential for the development of bioc...Sugar palm(Arenga pinnata)starch is considered an important renewable,biodegradable,and eco-friendly polymer,which is derived from agricultural by-products and residues,with great potential for the development of biocomposite materials.This research was aimed at investigating the development of TPS biocomposites from A.pinnata palm starch using an extrusion process.Palm starch,glycerol,and stearic acid were extruded in a twin-screw extruder.Scanning electron microscopy(SEM)analysis of TPS showed that the starch granules were damaged and gelatinized in the extrusion process.The density of TPS was 1.3695 g/mL,lower than that of palm starch,and the addition of stearic acid resulted in increased TPS density.X-ray diffraction(XRD)results showed that palm starch had a C-type pattern crystalline structure.The tensile strength,elongation at break,and modulus of elasticity of TPS were 7.19 MPa,33.95%,and 0.56 GPa,respectively.The addition of stearic acid reduced the tensile strength,elongation at break and modulus of elasticity of TPS.The rheological properties,i.e.,melt flow rate(MFR)and viscosity of TPS,were 7.13 g/10 min and 2482.19 Pa.s,respectively.The presence of stearic acid in TPS resulted in increased MFR and decreased viscosity values.The peak gelatinization temperature of A.pinnata palm starch was 70°C,while Tg of TPS was 65°C.The addition of stearic acid reduced the Tg of TPS.The thermogravimetric analysis(TGA)analysis showed that the addition of glycerol and stearic acid decreased the thermal stability,but extended the temperature range of thermal degradation.TPS derived from A.pinnata palm starch by extrusion method has the potential to be applied in industrial practice as a promising raw material for manufacturing bio-based packaging as a sustainable and green alternative to petroleum-based plastics.展开更多
Microplastics(<5 mm)are ubiquitous in the environment and can pose potential danger to the ecosystem and even human health.As the sink of microplastics,the ocean,especially the densely populated coastal area,has be...Microplastics(<5 mm)are ubiquitous in the environment and can pose potential danger to the ecosystem and even human health.As the sink of microplastics,the ocean,especially the densely populated coastal area,has become a hotspot for research on microplastic pollution.In the last decade,the research of marine microplastics has been rapidly increasing in China.This review summarized the microplastic research conducted in China marine waters so far,and introduced the trends and progress of microplastic research in the four seas along the coast of China.We reviewed and compared the current sampling,extraction,and identification methodologies of China's microplastic research.According to the sampling method,the 30 reviewed studies were separated into two categories,trawl sampling and bulk sampling,to summarize relevant data,including abundance,sizes,shapes,colors and polymer types of microplastics.The main results showed that the distribution of microplastics in China's marine environment varied significantly,with offshore mariculture zones and the South China Sea being the most contaminated areas.Transparent,granules(or pellets)and fibers were the most dominant microplastic colors and shapes,and the size of microplastics was influenced significantly by the sampling method.Polyethylene(PE),polypropylene(PP)and polystyrene(PS)were the most common polymer types found in the China Sea,accounting for 49.96%,29.97%,and 12.38%of the total studies,respectively.Compared with other global data,China's coastal microplastic pollution is at an intermediate level and does not seem to be a major microplastic pollution source.展开更多
Wetlands have long been used as environmental indicators for changes in climate and land use because they are sensitive to hydrologic change;however,wetlands set in transmissive groundwater can be more resilient to cl...Wetlands have long been used as environmental indicators for changes in climate and land use because they are sensitive to hydrologic change;however,wetlands set in transmissive groundwater can be more resilient to climate and land use change.In Anoka County,Minnesota,USA,a monitoring network was established in 1997 and maintained by the Anoka Conservation District to the present day to assess wetland hydrologic response over time.We examined a combination of data including water level(stage)from these wetlands,precipitation from local gages,pan evaporation data,and historical land use,including a measure of runoff flashiness,using regression and k-mean analysis.Results did not detect any clear trends over a 25-year time period,though some p-values showed potential.A clear statistical trend in the measured hydrologic parameters would suggest exceedance beyond historical thresholds of natural hydrologic variation and alert the need to better protect wetlands and groundwater from anthropogenic stress.Study results may provide useful information to management and regulatory decisions for wetland systems set in sandy soils.This is particularly important for Anoka County because most wetlands are intrinsically connected to the surficial ASP(Anoka Sand Plain)Aquifer,which overlay vulnerable deeper aquifers used for domestic water supply.展开更多
Thailand has a huge variability of bast fiber plants,some of which have been little researched regarding their applicability in composites.Bast fiber(bundle)s from different species were investigated and incorporated ...Thailand has a huge variability of bast fiber plants,some of which have been little researched regarding their applicability in composites.Bast fiber(bundle)s from different species were investigated and incorporated into a polylactide(PLA)matrix by injection molding.Hemp and kenaf were used as well-studied fibers,while roselle,Fryxell and paper mulberry are less extensively characterized.Tensile strength,tensile modulus and interfacial shear strength(IFSS)of single fiber(bundle)s were highest for hemp,followed by kenaf,roselle,Fryxell and paper mulberry.Despite the lower tensile strength and IFSS of paper mulberry,the highest tensile strength was achieved for the paper mulberry/PLA composite followed by hemp/PLA.Scanning electron microscope(SEM)analyses showed that the single cells in paper mulberry fiber bundles,in contrast to the other fiber types investigated,were only partially bonded to each other,which explains the lower strength of the fiber bundles.The higher aspect ratio of fiber(bundle)s of paper mulberry in the PLA composite can explain the good composite characteristics.Apart from hemp,paper mulberry shows the best reinforcing effect in the PLA matrix and offers interesting potential for composite applications.Compared to neat PLA,the tensile strength could be increased by 24%and the tensile modulus by 54%.展开更多
Black liquor is obtained as a by-product of the pulping process,which is used to convert biomass into pulp by removing lignin,hemicelluloses and other extractives from wood to free cellulose fibers.Lignin represents a...Black liquor is obtained as a by-product of the pulping process,which is used to convert biomass into pulp by removing lignin,hemicelluloses and other extractives from wood to free cellulose fibers.Lignin represents a major constituent in black liquor,with quantities varying from 20%to 30%,of which a very low share is used for manufacturing value-added products,while the rest is mainly burned for energy purposes,thus underestimating its great potential as a raw material.Therefore,it is essential to establish new isolation and extraction methods to increase lignin valorization in the development of bio-based chemicals.The aim of this research work was to determine the effect of KOH or ethanol concentration as an isolation agent on lignin yields and the chemical characteristics of lignin isolated from formacell black liquor of oil palm empty fruit bunch(OPEFB).Isolation of lignin was carried out using KOH with various concentrations ranging from 5%to 15%(w/v).Ethanol was also used to precipitate lignin from black liquor at concentrations varying from 5%to 30%(v/v).The results obtained showed that the addition of KOH solution at 12.5%and 15%concentrations resulted in better lignin yield and chemical properties of lignin,i.e.,pH values of 3.86 and 4.27,lignin yield of 12.78%and 14.95%,methoxyl content of 11.33%and 10.13%,and lignin equivalent weights of 476.25 and 427.03,respectively.Due to its phenolic structure and rich functional groups that are favorable for modifications,lignin has the potential to be used as a green additive in the development of advanced biocomposite products in various applications to replace current fossil fuel-based material,ranging from fillers,fire retardants,formaldehyde scavengers,carbon fibers,aerogels,and wood adhesives.展开更多
Grafting is a plant propagation technique widely used in agriculture.A recent discovery of the capability of interfamily grafting in Nicotiana has expanded the potential combinations of grafting.In this study,we showe...Grafting is a plant propagation technique widely used in agriculture.A recent discovery of the capability of interfamily grafting in Nicotiana has expanded the potential combinations of grafting.In this study,we showed that xylem connection is essential for the achievement of interfamily grafting and investigated the molecular basis of xylem formation at the graft junction.Transcriptome and gene network analyses revealed gene modules for tracheary element(TE)formation during grafting that include genes associated with xylem cell differentiation and immune response.The reliability of the drawn network was validated by examining the role of the Nicotiana benthamiana XYLEM CYSTEINE PROTEASE(NbXCP)genes in TE formation during interfamily grafting.Promoter activities of NbXCP1 and NbXCP2 genes were found in differentiating TE cells in the stem and callus tissues at the graft junction.Analysis of a Nbxcp1;Nbxcp2 loss-of-function mutant indicated that NbXCPs control the timing of de novo TE formation at the graft junction.Moreover,grafts of the NbXCP1 overexpressor increased the scion growth rate as well as the fruit size.Thus,we identified gene modules for TE formation at the graft boundary and demonstrated potential ways to enhance Nicotiana interfamily grafting.展开更多
文摘Sea cucumber saponins have attracted more attention in recent years due to biological activities.It is a popular practice to soak sea cucumber in Baijiu at home and being applied to industrial manufacturing in China.However,knowledge of the effect of alcohol on the absorption and metabolism of sea cucumber saponins is limited.The effects of alcohol on digestion,absorption and metabolism of sea cucumber saponins in BALB/c mice were investigated after gavage and tail intravenous injection.The results showed that the content of saponins in serum and liver was significantly higher under the influence of alcohol than that in the control group after oral administration.Alcohol promoted the absorption of sea cucumber saponins prototype as well as inhibited the process of saponins being transformed into deglycositic metabolites in the small intestine.Moreover,sea cucumber saponins remained in circulation for a long time and alcohol slowed down the clearance of sea cucumber saponins under the influence of alcohol after intravenous injection.This confirmed the feasibility of marinating sea cucumber in Baijiu to improve the efficacy of saponins and provides an important theoretical basis for the utilization of sea cucumber and the development of sea cucumber liquor.
基金supported by the National Natural Science Foundation of China(32072145)。
文摘Trimethylamine N-oxide(TMAO)is a risk factor of various chronic diseases,which was produced by metabolism from precursors to trimethylamine(TMA)in gut and the oxidation from TMA in liver.The TMA generation was influenced by diet,mainly due to the rich TMAO precursors in diet.However,it was still unclear that the effects of different proportion and source of macronutrients in different dietary pattern on the production process of TMAO.Here,the generation of TMA from precursors and TMAO from TMA was determined after single oral choline chloride and intraperitoneal injection TMA,respectively,in mice fed with carbohydrates,proteins and fats in different proportion and sources.The results suggested that the generation of TMAO was increased by low non-meat protein and high fat via enhancing the production of TMAO from TMA,and decreased by plant protein and refined sugar via reducing TMA production from precursors in gut and TMAO transformation from TMA in liver.The high fat and high sugar diets accelerating the development of atherosclerosis did not increase the production of TMAO,the risk factor for atherosclerosis,which indicated that the dietary compositions rather than the elevated TMAO level might be a more key risk factor for atherosclerosis.
基金supported by the National Natural Science Foundation of China(31922072)the Natural Science Foundation of Shandong Province(ZR2020JQ15)the Taishan Scholar Project of Shandong Province(tsqn201812020)。
文摘Sensitive monitoring of the target products during the biosynthesis process is crucial,and facile analytical approaches are urgently needed.Herein,phosphatidylserine(PS)was chosen as the model target,a colorimetric aptasensor was developed for the rapid quantitation in biosynthesis samples.A chimeric aptamer was constructed with two homogeneous original PS aptamers.Specific recognition between the chimeric aptamer and PS results in the desorption of aptamer from the surface of the AuNPs nanozyme,and the peroxidase-like enzymatic activity of the AuNPs nanozyme was weakened in a relationship with the different concentrations.The developed aptasensor performed well when applied for analyzing PS in biosynthesis samples.The aptasensor offers good sensitivity and selectivity,under optimal conditions,achieving monitoring and quantitation of PS in the range of 2.5-80.0μmol/L,with a limit of detection at 536.2 nmol/L.Moreover,the aptasensor provides good accuracy,with comparison rates of 98.17%-106.40%,when compared with the HPLC-ELSD.This study provides a good reference for monitoring other biosynthesized products and promoting the development of aptamers and aptasensors in real-world applications.
基金supported by the National Natural Science Foundation of China(No.41806167)the High-Level Talents Research Fund of Qingdao Agricultural University(No.665/1120034)+4 种基金the NSFC-Shandong Joint Fund(No.U1906212)the Major Project of the 14th Five-Year Plan(No.2022QNLM030003-1)the Natural Science Foundation of Shandong Province(No.ZR2021ZD28)the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(No.2021CXLH0012)the Youth Innovation Plan of Shandong Province(No.2019KJM004).
文摘Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum HDN11-186.Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass spectra.The absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)spectrum.UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured fungi.Compound 1 showed obvious antibacterial activities against Pro-teus sp.MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).
基金financially supported by the National Natural Science Foundation of China(22368014)the Guizhou Provincial S&T Project(ZK[2022]011,GCC[2023]011)+1 种基金the Guizhou Provincial Higher Education Institution Program(Qianjiaoji[2023]082)supported by RUDN University Strategic Academic Leadership Program。
文摘Renewable electrocatalytic upgrading of biomass feedstocks into valuable chemicals is one of the promising strategies to relieve the pressure of traditional energy-based systems.Through electrocatalytic carbon–carbon bond cleavage of high selectivity,various functionalized molecules,such as organic acids,amides,esters,and nitriles,have great potential to be accessed from biomass.However,it has merely received finite concerns and interests in the biorefinery.This review first showcases the research progress on the electrocatalytic conversion of lipid/sugar-and lignin-derived molecules(e.g.,glycerol,mesoerythritol,xylose,glucose,1-phenylethanol,and cyclohexanol)into organic acids via specific carbon–carbon bond scission processes,with focus on disclosing reaction mechanisms,recognizing actual active species,and collecting feasible modification strategies.For the guidance of further extensive studies on biomass valorization,organic transformations via a variety of reactions,including decarboxylation,ring-opening,rearrangement,reductive hydrogenation,and carboxylation,are also disclosed for the construction of similar carbon skeletons/scaffolds.The remaining challenges,prospective applications,and future objectives in terms of biomass conversion are also proposed.This review is expected to provide references to develop renewed electrocatalytic carbon–carbon bond cleavage transformation paths/strategies for biomass upgrading.
文摘The aim of this research work was to evaluate the potential of using renewable natural feedstock,i.e.,Jatropha curcas oil(JCO)for the synthesis of non-isocyanate polyurethane(NIPU)resin for wood composite applications.Commercial polyurethane(PU)is synthesized through a polycondensation reaction between isocyanate and poly-ol.However,utilizing toxic and unsustainable isocyanates for obtaining PU could contribute to negative impacts on the environment and human health.Therefore,the development of PU from eco-friendly and sustainable resources without the isocyanate route is required.In this work,tetra-n-butyl ammonium bromide was used as the activator to open the epoxy ring with 3-Aminopropyltriethoxisylane as a catalyst to yield urethane of JCO(UJCO).The UJCO were characterized by Fourier Transform Infra-Red spectroscopy(FTIR)and their oxirane,and hydroxyl values were measured.The result showed that a decrease in oxirane value was found while the hydroxyl value was increased during the time,confirming that the urethane group was formed.The presence of functional groups in FTIR spectra at wave numbers 1732.08,1562.34,and 3348.42 cm^(−1) indicates the functional groups of C=O(urethane carbonyl),–NH,and–OH,respectively confirmed this finding.The potential applications of NIPU in the wood composite were also outlined.
基金the China Agriculture Research System of MOF and MARA(No.CARS-48)the Taishan Scholar Project of Shandong Province(No.tsqn201812020)。
文摘In this study,we proposed a reliable and sustainable technique for the clean utilization of shrimp wastes,which can yield a solid inoculant of Bacillus subtilis OKF04 containing micronutrients at low cost without the risk of contamination.Study of the culture conditions revealed that the head of shrimp Litopenaus vannamei and the wheat bran acted as suitable substrates for the growth of B.subtilis OKF04.With 60%initial moisture content,30℃culture temperature,and 5%inoculation amount,followed by 48 hours of fermentation and 0.5%soluble starch added during the drying process(50℃for 6h),a solid B.subtilis OKF04 inoculant with a spore amount of 2.4×10^(10)CFU g^(-1)and a high amino acid content was obtained.The solid B.subtilis OKF04 inoculant was applied to cultivate pakchoi under pot experiment.As the result,of adding to,the size of stems and leaves,nutritional composition,and physiological activity of pakchoi were significantly(P<0.05)enhanced by solid B.subtilis OKF04 inoculant.B.subtilis OKF04 also significantly(P<0.05)increased the soil’s nutrient content and improved its microbial composition.Furthermore,pakchoi cultivated with a low dose of solid B.subtilis OKF04 inoculant(0.05 g kg^(-1)soil)resulted in the best results.This study provides a new method for the preparation of microbial inoculants with solid waste shrimp heads.
基金support from the National Natural Science Foundation of China(21776101)the National Natural Science Foundation of China(22178122)for this work.
文摘Adsorption dynamics of ethane in two granular fixed beds and structured fixed beds with microfibrous composites was studied.5A zeolite membrane 5A/PSSF(paper-like sintered stainless steel fiber)and microfibrous entrapped activated carbon(MEAC)composites were prepared by wet layup papermaking/sintering technique and in-situ hydrothermal method.Microfibrous composites were characterized by X-ray diffraction,scanning electron microscopy and N2 adsorption/desorption.Structured fixed beds were designed by filling granular adsorbents(5A zeolite or activated carbon)and microfibrous composites at the inlet and outlet of the beds,respectively.Effects of flow rate,bed height and structure on the breakthrough curves were investigated.The length of unused bed(LUB)was determined,and Yoon–Nelson model was used to fit the breakthrough curves.The experimental results showed ethane was effectively adsorbed on the granular adsorbents and microfibrous composites.Both composites could decrease the LUB values and enhance bed utilization.All breakthrough curves fitted well to Yoon–Nelson model,with correlation coefficient exceeding 0.89.The adsorption rate of ethane could be improved in the structured fixed beds,which showed an enhanced mass transfer efficiency for ethane adsorption.LUB values of structured fixed beds with 5A/PSSF composites were larger,the bed utilization values were lower,and the adsorption rate constants were higher than those with MEAC composites under the same conditions.
基金supported by the National Key R&D Program of China(No.2018YFC0311203)the Fundamental Research Funds for the Central Universities(No.201941005).
文摘Laminarinases reveal potential application in the field of food and biotechnology.In this research,a novel GH16 family laminarinase,designated as Lam16A_Wa,was cloned from the genome of marine bacterium Wenyingzhuangia aestuarii OF219 and expressed in Escherichia coli.Lam16A_Wa demonstrates a relatively low optimal reaction temperature(35℃)and a cold-adapted feature.Its optimal pH value is 6.0 and is stable in a broad pH range from 3.0 to 11.0.A glycomics strategy was employed to investigate the hydrolytic pattern of Lam16A_Wa.The enzyme was confirmed as a random endo-acting glycoside hydrolase.Its minimum substrate was laminarin pentasaccharide,and the major final products are oligosaccharides,including disaccharide to pentasaccharide.The Lam16A_Wa provides a novel and well-defined tool for the molecular tailoring of laminarin.
基金supported by the National Natural Science Foundation of China(31922072)Natural Science Foundation of Shandong Province(ZR2020JQ15)+3 种基金Project of Shandong Province Higher Educational Science and Technology Program(2019KJF012)Taishan Scholar Project of Shandong Province(tsqn201812020)China Agriculture Research System(CARS-48)Qingdao Science and Technology Demonstration and Guidance Project for Benefiting the People(20-3-4-28-nsh)。
文摘Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia coli.To enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)mutants.To test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for expression.The enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 promoter.In high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 promoter.Therefore,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.
基金financial supports granted by the National Natural Science Foundation of China(Nos.82073759 and 82003583)the Fund of Greater Bay Area Institute of Precision Medicine(Guangzhou)(No.IPM2021C009)the National Science and Technology Major Project for Significant New Drugs Development(No.2018ZX09735004)。
文摘The Janus kinases(JAKs)are a family of intracellular tyrosine kinases that play an essential role in many basic biological processes,such as apoptosis and inflammation.Thus any dysfunction of the proteins in this pathway may lead to a variety of diseases,including cancer and diseases that affect the immune system,such as severe combined immune deficient(SCID).Marine biological resources have become an important source in new drug research and development due to their diversity,complexity and speciality.In this study,Marine alkaloid Neobacillamide A was isolated from the greedy and stubborn sponge symbiotic Bacillus atrophicus C89 in the South China Sea.Totally 24 novel marine alkaloid Neobacillamide A derivatives were designed and synthesized,which were evaluated for their inhibitory activity against JAK/STAT signaling pathway and their cytotoxicity to A549 cells.Compounds 13c,13o,14d,14g and 14h showed potent JAK/STAT inhibition capability(concentration of 25μmol L^(-1),all inhibitory potencies were above 60%),especially compound 14g exhibited superior JAK/STAT inhibition effect(89.70%inhibition).In addition,all these compounds with a concentration of 25μmol L^(-1)displayed weak or no cytotoxicity to A549 cells,which means that these Neobacillamide A derivatives with JAK/STAT inhibition capability may have potential anti-inflammatory function.
基金funded by the Fundamental Research Funds for the Central Universities(Nos.201912008,201964019)the Natural Science Foundation of Shandong Province(No.ZR2019BC025).
文摘A sensitive,rapid,and robust ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was established for the first time to quantify agarotriose(A3)in rat plasma,tissues,urine,and feces.A3 and stachyose(internal standard)were separated by a BEH amide column at 65℃under the mobile phase of 10 mmol L^(-1)ammonium ace-tate-acetonitrile(42:58,v/v)with 350µLmin-1.The acquisition of transitions was carried out in multiple reaction monitoring(MRM)pattern operating with positive ionization at m/z 509.16>329.15 for A3 and m/z 689.15>527.11 for stachyose.The linearity ranges of A3 were 10 to 5000nmolL^(-1)for plasma,20 to 10000nmolL^(-1)for tissues,and 40 to 20000nmolL^(-1)for urine and feces.The accuracy and precision ranged from 90.9%to 111.6%and 0.7%to 10.1%,respectively.The stability was between 86.1%and 102.5%.The extraction recovery was consistent and reproducible.The matrix effect ranged from 1.5%to 11.4%.The pharmacokinetic,tissue dis-tribution,and excretion studies were successfully conducted with the validated method.Results showed that A3 could be absorbed by rats,and the absolute bioavailability was 6.7%.Furthermore,it was rapidly distributed in rat tissues and mainly eliminated via feces excretion(67.0%)after oral administration.For intravenous bolus,85.5%was recovered,and renal excretion was the primary path-way(77.6%)for cumulative recovery.
基金supported by the National Natural Science Foundation of China(Nos.81673350,81703411,41776156)the National Science and Technology Major Project for Significant New Drugs Development,China(No.2018ZX09735-004)+1 种基金the Fundamental Research Funds for the Central Universities of China(No.201962002)the Taishan Scholars Program,China.
文摘Chemical epigenetic manipulation was applied to the zoanthid-derived fungus Cochliobolus lunatus(TA26-46)with a histone deacetylation modifier(100μmol L^(−1) nicotinamide),resulting in the isolation of a new 14-membered resorcylic acid lactone named 7′(Z)-zeaenol(1),together with six known analogues(2−7)from the treated broth.The planar structure of 1 was determined by comprehensive NMR spectroscopy and HRESIMS data.The absolute configuration of 1 was elucidated by ECD spectrum,^(13)C NMR shift calculations,and on the basis of biogenetic considerations.Compound 5 exhibited cytotoxic activity against the human tumor cell lines A549,HCT-116,HT-29,Hela,MCF-7,and K562 with the IC_(50) values ranging from 2.54 to 7.44μmolL^(−1).
基金supported by the National Natural Science Foundation of China(Nos.42176126,42076221)the Department of Marine Strategic Planning and Economy,Ministry of Natural Resources of China,and Marine Development Research Society of China(No.CAMA201817).
文摘The marine biopharmaceutical industry(MBI)has been considered as an important part of the blue economy.The high-quality development of this industry depends on the high-level coordinated development of technological innovation system(TIS).In the present study,the coupling mechanism of industrial innovation input subsystem and innovation output subsystem was analyzed for the first time.On this basis,the development level and coupling coordination level of TIS in China’s MBI during 2008-2018 were empirically evaluated with the capacity coupling coordination model.Then,the obstacle factors were diagnosed and recognized with the obstacle model.The results showed that the innovation input index fluctuated at a low level in China’s MBI.The innovation output index has basically maintained a growth trend,whereas the quality of development was not high.Although the coupling coordination level of TIS showed a positive change as mild disordered→primary coordinated→well-coordinated,the development type of innovation system has changed from the lagging output of innovation into the lagging input of innovation.Insufficient input of innovation factors remained the main obstacle to the improvement of coordination level.Based on the above analysis,suggestions were put forward from the perspectives of policy and fund guarantees to improve the coupling coordination level in China’s MBI.
基金from The Hitachi Global Foundation Asia Innovation Award 2020.Also,the authors thank the facilities,scientific and technical support from Advanced Characterization Laboratories Serpong and Cibinong,National Research and Innovation Institute through E-Layanan Sains,Badan Riset dan Inovasi Nasional(BRIN).
文摘Sugar palm(Arenga pinnata)starch is considered an important renewable,biodegradable,and eco-friendly polymer,which is derived from agricultural by-products and residues,with great potential for the development of biocomposite materials.This research was aimed at investigating the development of TPS biocomposites from A.pinnata palm starch using an extrusion process.Palm starch,glycerol,and stearic acid were extruded in a twin-screw extruder.Scanning electron microscopy(SEM)analysis of TPS showed that the starch granules were damaged and gelatinized in the extrusion process.The density of TPS was 1.3695 g/mL,lower than that of palm starch,and the addition of stearic acid resulted in increased TPS density.X-ray diffraction(XRD)results showed that palm starch had a C-type pattern crystalline structure.The tensile strength,elongation at break,and modulus of elasticity of TPS were 7.19 MPa,33.95%,and 0.56 GPa,respectively.The addition of stearic acid reduced the tensile strength,elongation at break and modulus of elasticity of TPS.The rheological properties,i.e.,melt flow rate(MFR)and viscosity of TPS,were 7.13 g/10 min and 2482.19 Pa.s,respectively.The presence of stearic acid in TPS resulted in increased MFR and decreased viscosity values.The peak gelatinization temperature of A.pinnata palm starch was 70°C,while Tg of TPS was 65°C.The addition of stearic acid reduced the Tg of TPS.The thermogravimetric analysis(TGA)analysis showed that the addition of glycerol and stearic acid decreased the thermal stability,but extended the temperature range of thermal degradation.TPS derived from A.pinnata palm starch by extrusion method has the potential to be applied in industrial practice as a promising raw material for manufacturing bio-based packaging as a sustainable and green alternative to petroleum-based plastics.
基金the National Natural Science Foundation of China(No.42176239)the Asian Countries Maritime Cooperation Fund(No.99950410)the Investigation and Evaluation of Microplastics in Seawater(No.ZY0722044)。
文摘Microplastics(<5 mm)are ubiquitous in the environment and can pose potential danger to the ecosystem and even human health.As the sink of microplastics,the ocean,especially the densely populated coastal area,has become a hotspot for research on microplastic pollution.In the last decade,the research of marine microplastics has been rapidly increasing in China.This review summarized the microplastic research conducted in China marine waters so far,and introduced the trends and progress of microplastic research in the four seas along the coast of China.We reviewed and compared the current sampling,extraction,and identification methodologies of China's microplastic research.According to the sampling method,the 30 reviewed studies were separated into two categories,trawl sampling and bulk sampling,to summarize relevant data,including abundance,sizes,shapes,colors and polymer types of microplastics.The main results showed that the distribution of microplastics in China's marine environment varied significantly,with offshore mariculture zones and the South China Sea being the most contaminated areas.Transparent,granules(or pellets)and fibers were the most dominant microplastic colors and shapes,and the size of microplastics was influenced significantly by the sampling method.Polyethylene(PE),polypropylene(PP)and polystyrene(PS)were the most common polymer types found in the China Sea,accounting for 49.96%,29.97%,and 12.38%of the total studies,respectively.Compared with other global data,China's coastal microplastic pollution is at an intermediate level and does not seem to be a major microplastic pollution source.
文摘Wetlands have long been used as environmental indicators for changes in climate and land use because they are sensitive to hydrologic change;however,wetlands set in transmissive groundwater can be more resilient to climate and land use change.In Anoka County,Minnesota,USA,a monitoring network was established in 1997 and maintained by the Anoka Conservation District to the present day to assess wetland hydrologic response over time.We examined a combination of data including water level(stage)from these wetlands,precipitation from local gages,pan evaporation data,and historical land use,including a measure of runoff flashiness,using regression and k-mean analysis.Results did not detect any clear trends over a 25-year time period,though some p-values showed potential.A clear statistical trend in the measured hydrologic parameters would suggest exceedance beyond historical thresholds of natural hydrologic variation and alert the need to better protect wetlands and groundwater from anthropogenic stress.Study results may provide useful information to management and regulatory decisions for wetland systems set in sandy soils.This is particularly important for Anoka County because most wetlands are intrinsically connected to the surficial ASP(Anoka Sand Plain)Aquifer,which overlay vulnerable deeper aquifers used for domestic water supply.
基金The present work was funded within the framework of the BMBF Exchange Project“Thai-German Agro-Based Fiber Exchange Programme-Sustainable Development:From Plant to Product(Acronym:AgroFiber)”under Registration No.01DP15016.
文摘Thailand has a huge variability of bast fiber plants,some of which have been little researched regarding their applicability in composites.Bast fiber(bundle)s from different species were investigated and incorporated into a polylactide(PLA)matrix by injection molding.Hemp and kenaf were used as well-studied fibers,while roselle,Fryxell and paper mulberry are less extensively characterized.Tensile strength,tensile modulus and interfacial shear strength(IFSS)of single fiber(bundle)s were highest for hemp,followed by kenaf,roselle,Fryxell and paper mulberry.Despite the lower tensile strength and IFSS of paper mulberry,the highest tensile strength was achieved for the paper mulberry/PLA composite followed by hemp/PLA.Scanning electron microscope(SEM)analyses showed that the single cells in paper mulberry fiber bundles,in contrast to the other fiber types investigated,were only partially bonded to each other,which explains the lower strength of the fiber bundles.The higher aspect ratio of fiber(bundle)s of paper mulberry in the PLA composite can explain the good composite characteristics.Apart from hemp,paper mulberry shows the best reinforcing effect in the PLA matrix and offers interesting potential for composite applications.Compared to neat PLA,the tensile strength could be increased by 24%and the tensile modulus by 54%.
基金This work was also supported by the Project“Development,Properties,and Application of Eco-Friendly Wood-Based Composites”,No.HИC-Б-1145/04.2021,carried out at the University of Forestry,Sofia,Bulgaria.The authors would like to acknowledge the Fundamental Research Grant Scheme(FRGS 2018-1)Reference Code:FRGS/1/2018/WAB07/UPM/1 provided by the Ministry of Higher Education,Malaysia.
文摘Black liquor is obtained as a by-product of the pulping process,which is used to convert biomass into pulp by removing lignin,hemicelluloses and other extractives from wood to free cellulose fibers.Lignin represents a major constituent in black liquor,with quantities varying from 20%to 30%,of which a very low share is used for manufacturing value-added products,while the rest is mainly burned for energy purposes,thus underestimating its great potential as a raw material.Therefore,it is essential to establish new isolation and extraction methods to increase lignin valorization in the development of bio-based chemicals.The aim of this research work was to determine the effect of KOH or ethanol concentration as an isolation agent on lignin yields and the chemical characteristics of lignin isolated from formacell black liquor of oil palm empty fruit bunch(OPEFB).Isolation of lignin was carried out using KOH with various concentrations ranging from 5%to 15%(w/v).Ethanol was also used to precipitate lignin from black liquor at concentrations varying from 5%to 30%(v/v).The results obtained showed that the addition of KOH solution at 12.5%and 15%concentrations resulted in better lignin yield and chemical properties of lignin,i.e.,pH values of 3.86 and 4.27,lignin yield of 12.78%and 14.95%,methoxyl content of 11.33%and 10.13%,and lignin equivalent weights of 476.25 and 427.03,respectively.Due to its phenolic structure and rich functional groups that are favorable for modifications,lignin has the potential to be used as a green additive in the development of advanced biocomposite products in various applications to replace current fossil fuel-based material,ranging from fillers,fire retardants,formaldehyde scavengers,carbon fibers,aerogels,and wood adhesives.
基金This work was supported by grants from the Japan Society for the Promotion of Science Grantsin-Aid for Scientific Research(20H03273,21H00368 and 21H05657 toMNand 22 K06181 to KK)Japan Science and Technology Agency(JPMJTR194G to MN)+1 种基金China Scholarship Council(CSCNo.201908050204 to CH).
文摘Grafting is a plant propagation technique widely used in agriculture.A recent discovery of the capability of interfamily grafting in Nicotiana has expanded the potential combinations of grafting.In this study,we showed that xylem connection is essential for the achievement of interfamily grafting and investigated the molecular basis of xylem formation at the graft junction.Transcriptome and gene network analyses revealed gene modules for tracheary element(TE)formation during grafting that include genes associated with xylem cell differentiation and immune response.The reliability of the drawn network was validated by examining the role of the Nicotiana benthamiana XYLEM CYSTEINE PROTEASE(NbXCP)genes in TE formation during interfamily grafting.Promoter activities of NbXCP1 and NbXCP2 genes were found in differentiating TE cells in the stem and callus tissues at the graft junction.Analysis of a Nbxcp1;Nbxcp2 loss-of-function mutant indicated that NbXCPs control the timing of de novo TE formation at the graft junction.Moreover,grafts of the NbXCP1 overexpressor increased the scion growth rate as well as the fruit size.Thus,we identified gene modules for TE formation at the graft boundary and demonstrated potential ways to enhance Nicotiana interfamily grafting.