期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Detection of Breast Cancer 1 (BRCA1) Gene Using an Electrochemical DNA Biosensor Based on Immobilized ZnO Nanowires 被引量:1
1
作者 Nur Azimah Mansor Zainiharyati Mohd Zain +4 位作者 Hairul Hisham Hamzah Mohd Shihabuddin Ahmad Noorden Siti Safura Jaapar Valerio Beni Zafar Husain Ibupoto 《Open Journal of Applied Biosensor》 2014年第2期9-17,共9页
Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5’ AAT GGA TTT ATC TGC TCT TCG 3’) specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is bas... Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5’ AAT GGA TTT ATC TGC TCT TCG 3’) specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is based on short oligonucleotide DNA probe immobilized onto zinc oxide nanowires (ZnONWs) chemically synthesized onto gold electrode via hydrothermal technique. The morphology studies of the ZnONWs, performed by field emission scanning electron microscopy (FESEM), showed that the ZnO nanowires are uniform, highly dense and oriented perpendicularly to the substrate. Recognition event between the DNA probe and the target was investigated by differential pulse voltammetry (DPV) in 0.1 M acetate buffer solution (ABS), pH 7.00;as a result of the hybridization, an oxidation signal was observed at +0.8 V. The influences of pH, target concentration, and non-complimentary DNA on biosensor performance were examined. The proposed DNA biosensor has the ability to detect the target sequence in the range of concentration between 10.0 and 100.0 μM with a detection limit of 3.32 μM. The experimental results demonstrated that the prepared ZnONWs/Au electrodes are suitable platform for the immobilization of DNA. 展开更多
关键词 Zinc Oxide Nanowires DNA Biosensor Breast Cancer Gene BRCA1 DNA Hybridization Differential Pulse Voltammetry
下载PDF
Geometric Flow Control Lateral Flow Immunoassay Devices (GFC-LFIDs): A New Dimension to Enhance Analytical Performance
2
作者 E.Eriksson J.Lysell +3 位作者 H.Larsson K.Y.Cheung D.Filippini W.C.Mak 《Research》 EI CAS 2019年第1期851-858,共8页
Te nitrocellulose(NC)membrane based lateral fow immunoassay device(LFID)is one of the most important and widely used biosensor platforms for point-of-care(PoC)diagnostics.However,the analytical performance of LFID has... Te nitrocellulose(NC)membrane based lateral fow immunoassay device(LFID)is one of the most important and widely used biosensor platforms for point-of-care(PoC)diagnostics.However,the analytical performance of LFID has limitations and its optimization is restricted to the bioassay chemistry,the membrane porosity,and the choice of biolabel system.Tese bottom neck technical issues resulted from the fact that the conventional LFID design principle has not evolved for many years,which limited the LFID for advanced biosensor applications.Here we introduce a new dimension for LFID design and optimization based on geometric fow control(GFC)of NC membranes,leading to highly sensitive GFC-LFID.Tis novel approach enables comprehensive fow control via diferent membrane geometric features such as the width(θ)and the length(l)of a constriction,as well as its input angle(θ_(1))and output angle(θ_(2)).Te GFC-LFID(θ=0.5 mm,l=7 mm,θ_(1)=60∘,θ_(2)=45∘)attained a 10-fold increase in sensitivity for detection of interleukin-6(IL-6),compared with conventional LFID,whereas reducing by 10-fold the antibody consumption.Te GFC-LFID detects IL-6 over a linear range of 0.1–10 ng/mL with a limit of detection(LoD)of 29 pg/mL,which even outperforms some commercial IL-6 LFIDs.Such signifcant improvement is attained by pure geometric control of the NC membrane,without additives,that only relaying on a simple high throughput laser ablation procedure suitable for integration on regular large-scale manufacturing of GFC-LFIDs.Our new development on GFC-LFID with the combination of facile scalable fabrication process,tailored fow control,improved analytical performance,and reduced antibodies consumption is likely to have a signifcant impact on new design concept for the LFID industry. 展开更多
关键词 DIMENSION optimization POROSITY
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部