Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study,repair and engineering of cardiac tissues.They are typically constructed from rigid and brittle materials process...Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study,repair and engineering of cardiac tissues.They are typically constructed from rigid and brittle materials processed in a cleanroom environment.An outstanding technological challenge is the integration of soft materials enabling a closer match to the mechanical properties of biological cells and tissues.Here we present an algorithm for direct writing of elastic membranes with embedded electrodes,optical waveguides and microfluidics using a commercial 3D printing system and a palette of silicone elastomers.As proof of principle,we demonstrate interfacing of cardiomyocytes derived from human induced pluripotent stem cells(hiPSCs),which are engineered to express Channelrhodopsin-2.We demonstrate electrical recording of cardiomyocyte field potentials and their concomitant modulation by optical and pharmacological stimulation delivered via the membrane.Our work contributes a simple prototyping strategy with potential applications in organ-on-chip or implantable systems that are multi-modal and mechanically soft.展开更多
基金We acknowledge funding from Volkswagen Foundation(Freigeist 91690)ERC starting grant(804005‐IntegraBrain)and Center for Advancing Electronics Dresden(cfaed).V.B.was supported by the Volkswagen Foundation(Freigeist A110720)by an ERC starting grant(678071-ProNeurons).
文摘Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study,repair and engineering of cardiac tissues.They are typically constructed from rigid and brittle materials processed in a cleanroom environment.An outstanding technological challenge is the integration of soft materials enabling a closer match to the mechanical properties of biological cells and tissues.Here we present an algorithm for direct writing of elastic membranes with embedded electrodes,optical waveguides and microfluidics using a commercial 3D printing system and a palette of silicone elastomers.As proof of principle,we demonstrate interfacing of cardiomyocytes derived from human induced pluripotent stem cells(hiPSCs),which are engineered to express Channelrhodopsin-2.We demonstrate electrical recording of cardiomyocyte field potentials and their concomitant modulation by optical and pharmacological stimulation delivered via the membrane.Our work contributes a simple prototyping strategy with potential applications in organ-on-chip or implantable systems that are multi-modal and mechanically soft.
