A label-free lactic acid sensor has been successfully developed by using a Dysprosium single crystal-based photoelectric potential technique via Dy-SCN/FTO electrode. Interestingly, the proposed sensor demonstrated ex...A label-free lactic acid sensor has been successfully developed by using a Dysprosium single crystal-based photoelectric potential technique via Dy-SCN/FTO electrode. Interestingly, the proposed sensor demonstrated excellent performance for L-lactic acid analysis with a wide linear range of 0.0196~16.31 mmol/L,the detection limit of as low as 3.20 μmol/L as well as an advisable stability. The feasibility of this strategy was also verified by practical application towards human sweat samples. The mechanism studies indicated that lactic acid molecules specifically bind to the surface of semiconductor materials, which alters the charge distribution of the electrode surface and subsequently results in band bending/photoelectric potential changes. The theoretical formula for this photoelectric chemistry(PEC) strategy was then derived according to charge balance theory. We believe that the proposed Dy-SCN/FTO sensor would open a new way for rapid, non-invasive L-lactic acid level evaluation during human physical condition monitoring.展开更多
Liquid chromatography tandem mass spectrometry(LC-MS/MS) plays an important role in clinical diagnostics. Although LC-MS/MS is superior in terms of accurately quantifying molecules in complex matrices,instrument footp...Liquid chromatography tandem mass spectrometry(LC-MS/MS) plays an important role in clinical diagnostics. Although LC-MS/MS is superior in terms of accurately quantifying molecules in complex matrices,instrument footprint, operation and maintenance complexity also hinder its expansion as the analytical technique of choice. In this study, a compact LC-MS instrument was developed, in which an assembled liquid chromatograph was coupled with a miniature ion trap mass spectrometer. The overall instrument has a footprint of 69 cm × 31 cm × 31 cm, and it requires no gas supply as well as minimum maintenance. Furthermore, the use of LC-MS is in accord with conventional clinical diagnostic protocols, and the choice of ion trap offers tandem MS performance. The results showed that the use of LC could improve both mixture analysis capability and detection sensitivity of the miniature mass spectrometer. After optimization, feasibility of this instrument in clinical practice was demonstrated by the quantitation of four widely used immunosuppressants in blood samples. Relatively good linearities were obtained, which spanned the reference ranges of effective therapeutic concentrations of each immunosuppressant. Intraday and inter-day accuracy and precision of analytical method were also assessed. This work showed that a compact LC-MS instrument could be used in clinical diagnosis, either to replace conventional lab-scale instruments or to be used in POCT applications.展开更多
基金the National Natural Science Foundation of China (Nos. 22172040 and 21974031)the Department of Science and Techniques of Guangdong Province (Nos. 2021A1515010180 and 2019B010933001)+1 种基金Guangzhou Municipal Science and Technology Bureau (No. 202102010449)Guangzhou Municipal Science and Technology Bureau (No. GZQC_(2)0-PZ11-FD084) for their financial support。
文摘A label-free lactic acid sensor has been successfully developed by using a Dysprosium single crystal-based photoelectric potential technique via Dy-SCN/FTO electrode. Interestingly, the proposed sensor demonstrated excellent performance for L-lactic acid analysis with a wide linear range of 0.0196~16.31 mmol/L,the detection limit of as low as 3.20 μmol/L as well as an advisable stability. The feasibility of this strategy was also verified by practical application towards human sweat samples. The mechanism studies indicated that lactic acid molecules specifically bind to the surface of semiconductor materials, which alters the charge distribution of the electrode surface and subsequently results in band bending/photoelectric potential changes. The theoretical formula for this photoelectric chemistry(PEC) strategy was then derived according to charge balance theory. We believe that the proposed Dy-SCN/FTO sensor would open a new way for rapid, non-invasive L-lactic acid level evaluation during human physical condition monitoring.
基金supported by the National Key Research and Development Program of China (No. 2020YFF01014502)the National Natural Science of Foundation of China (Nos. 21922401,201827810)。
文摘Liquid chromatography tandem mass spectrometry(LC-MS/MS) plays an important role in clinical diagnostics. Although LC-MS/MS is superior in terms of accurately quantifying molecules in complex matrices,instrument footprint, operation and maintenance complexity also hinder its expansion as the analytical technique of choice. In this study, a compact LC-MS instrument was developed, in which an assembled liquid chromatograph was coupled with a miniature ion trap mass spectrometer. The overall instrument has a footprint of 69 cm × 31 cm × 31 cm, and it requires no gas supply as well as minimum maintenance. Furthermore, the use of LC-MS is in accord with conventional clinical diagnostic protocols, and the choice of ion trap offers tandem MS performance. The results showed that the use of LC could improve both mixture analysis capability and detection sensitivity of the miniature mass spectrometer. After optimization, feasibility of this instrument in clinical practice was demonstrated by the quantitation of four widely used immunosuppressants in blood samples. Relatively good linearities were obtained, which spanned the reference ranges of effective therapeutic concentrations of each immunosuppressant. Intraday and inter-day accuracy and precision of analytical method were also assessed. This work showed that a compact LC-MS instrument could be used in clinical diagnosis, either to replace conventional lab-scale instruments or to be used in POCT applications.
