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塞内卡病毒多克隆抗体的制备及其在免疫组织化学中的初步应用
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作者 杨珍 江之瑶 +5 位作者 夏玉洁 马凤龙 李心清 郭亚飞 杨洁 李明义 《中国兽医杂志》 CAS 北大核心 2023年第8期72-76,共5页
为了制备纯度高、特异性强的塞内卡病毒(SVA)多克隆抗体并研究其在免疫组织化学(IHC)中的应用,本试验用甲醛溶液灭活SVA,将其与矿物油佐剂充分混合乳化后,接种兔制备多克隆抗体并测定中和抗体效价,经Protein A亲和层析介质纯化,用十二... 为了制备纯度高、特异性强的塞内卡病毒(SVA)多克隆抗体并研究其在免疫组织化学(IHC)中的应用,本试验用甲醛溶液灭活SVA,将其与矿物油佐剂充分混合乳化后,接种兔制备多克隆抗体并测定中和抗体效价,经Protein A亲和层析介质纯化,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴定抗体纯度,用斑点杂交法(Dot blot)鉴定抗体特异性;以该抗体作为一抗,应用IHC技术检测SVA感染猪的蹄部组织切片中抗原的表达情况。结果显示,制备的SVA多克隆抗体中和抗体效价为1∶1024,纯化后的抗体经SDS-PAGE鉴定仅有分子量约55 kDa和25 kDa两条目的蛋白条带;Dot blot结果显示,该抗体特异性强,最低检测浓度为0.15μg/mL;IHC结果显示,该抗体在1∶500稀释时能够检测到组织切片中抗原的表达。结果表明,本试验制备的SVA多克隆抗体符合IHC技术要求,可为下一步SVA致病机理研究提供重要的试验材料。 展开更多
关键词 塞内卡病毒 多克隆抗体 鉴定 免疫组织化学
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霍尔效应中的空穴 被引量:2
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作者 赵庆利 马连喜 《物理通报》 2008年第5期14-16,共3页
霍尔效应(Hall effect)可以用来测定金属和半导体中的载流子符号,霍尔系数定义为RH=Ey/jxH其中Ey,jx,H分别为电场、电流密度和磁场(图1).
关键词 霍尔效应 空穴 霍尔系数 电流密度 载流子 半导体 电场 磁场
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再论空穴和霍尔效应 被引量:1
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作者 马连喜 《物理通报》 2010年第5期67-67,共1页
笔者曾谈论过空穴和霍尔效应问题,试图回答,在本质上都是电子移动的情况下,为什么自由电子导电(n型半导体)和空穴导电(p型半导体)会出现相反方向的霍尔电压.笔者想用更通俗的方式再论述一下此问题.
关键词 霍尔效应 空穴 电子导电 P型半导体 N型半导体 霍尔电压
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Heat transfer characteristics of thin power-law liquid films over horizontal stretching sheet with internal heating and variable thermal coefficient
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作者 Yanhai LIN Liancun ZHENG Lianxi MA 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2016年第12期1587-1596,共10页
The effect of internal heating source on the film momentum and thermal transport characteristic of thin finite power-law liquids over an accelerating unsteady horizontal stretched interface is studied. Unlike most cla... The effect of internal heating source on the film momentum and thermal transport characteristic of thin finite power-law liquids over an accelerating unsteady horizontal stretched interface is studied. Unlike most classical works in this field, a general surface temperature distribution of the liquid film and the generalized Fourier's law for varying thermal conductivity are taken into consideration. Appropriate similarity transformations are used to convert the strongly nonlinear governing partial differential equations (PDEs) into a boundary value problem with a group of two-point ordinary differential equations (ODEs). The correspondence between the liquid film thickness and the unsteadiness parameter is derived with the BVP4C program in MATLAB. Numerical solutions to the self-similarity ODEs are obtained using the shooting technique combined with a Runge-Kutta iteration program and Newton's scheme. The effects of the involved physical parameters on the fluid's horizontal velocity and temperature distribution are presented and discussed. 展开更多
关键词 non-Newtonian fluid nonlinear equation thin film heat transfer internalheating stretching sheet thermal conductivity numerical solution
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Mechanism from particle compaction to fluidization of liquid–solid two-phase flow
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作者 Yue Zhang Jinchun Song +2 位作者 Lianxi Ma Liancun Zheng Minghe Liu 《Chinese Physics B》 SCIE EI CAS CSCD 2020年第1期373-377,共5页
A new model of particle yield stress including cohesive strength is proposed,which considers the friction and cohesive strength between particles.A calculation method for the fluidization process of liquid–solid two-... A new model of particle yield stress including cohesive strength is proposed,which considers the friction and cohesive strength between particles.A calculation method for the fluidization process of liquid–solid two-phase flow in compact packing state is given,and the simulation and experimental studies of fluidization process are carried out by taking the sand–water two-phase flow in the jet dredging system as an example,and the calculation method is verified. 展开更多
关键词 liquid–solid flow two-phase flow cohesive strength yield stress
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Threshold control in VCSELs by proton implanted depth 被引量:1
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作者 赵红东 孙梅 +4 位作者 王伟 马连喜 刘会丽 李文超 刘琦 《Optoelectronics Letters》 EI 2011年第4期263-265,共3页
The proton implantation is one of key procedures to confine the current diffusion in vertical cavity surface emitting lasers(VCSELs),in which the proton implanted depth and profile are main parameters.Threshold charac... The proton implantation is one of key procedures to confine the current diffusion in vertical cavity surface emitting lasers(VCSELs),in which the proton implanted depth and profile are main parameters.Threshold characteristics of VCSELs with various proton implanted depths are studied after optical,electrical and thermal fields have been simulated self-consistently in three dimensions.It is found that for VCSELs with confinement radius of 2 mm,increasing proton implanted depth can reduce the injected current threshold power and enhance the laser temperature in active region.Numerical results also indicate that there are optimal values for current aperture in proton implanted VCSELs.The minimum injected current threshold can be achieved in VCSELs with proton implantation near the active region and confinement radius of 1.5 mm,while the VCSELs with proton implantation in the middle of p-type distributed Bragg reflectors(DBRs) and confinement radius of 2.5 mm can realize the minimum temperature. 展开更多
关键词 PROTONS
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Analytical Solutions of a Model for Brownian Motion in the Double Well Potential
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作者 刘爱洁 郑连存 +1 位作者 马连喜 张欣欣 《Communications in Theoretical Physics》 SCIE CAS CSCD 2015年第1期51-56,共6页
In this paper, the analytical solutions of Schrodinger equation for Brownian motion in a double well potential are acquired by the homotopy analysis method and the Adomian decomposition method. Double well potential f... In this paper, the analytical solutions of Schrodinger equation for Brownian motion in a double well potential are acquired by the homotopy analysis method and the Adomian decomposition method. Double well potential for Brownian motion is always used to obtain the solutions of Fokker-P1anck equation known as the Klein-Kramers equation, which is suitable for separation and additive Hamiltonians. In essence, we could study the random motion of Brownian particles by solving Schr6dinger equation. The anaiytical results obtained from the two different methods agree with each other well The double well potentiai is affected by two parameters, which are analyzed and discussed in details with the aid of graphical illustrations. According to the final results, the shapes of the double well potential have significant influence on the probability density function. 展开更多
关键词 Brown motion homotopy analysis method Schrodinger equation double well potential
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