Electroacupuncture ameliorates cardiac dysfunction in myocardial ischemia model rats:a potential role of the hypothalamic-pituitary-adrenal axis

OBJECTIVE:To verify the hypothesis that electroacupuncture inhibits the hyperactivity of the hypothalamic-pituitary-adrenal(HPA)axis via regulating the expression of glial fibrillary acidic protein(GFAP)in the hippocampus of acute myocardial ischemia(AMI)rats.METHODS:Sixty-six healthy male Sprague-Dawley rats were randomly divided into five groups:Sham,AMI(Model),electroacupuncture at Shenmen(HT7)-Tongli(HT5)segment(EA),non-acupoint electroacupuncture(Control),and Model+corticosterone(Model+CORT).AMI was induced via occlusion of the left anterior descending coronary artery,followed by 3 d of electroacupuncture at Shenmen(HT7)-Tongli(HT5)segment.In the Control group,electroacupuncture was applied at points lying 5 and 10 mm from the base of the tail.The AMI+CORT group was injected with CORT(20 mg/kg)in saline.Hemorheology,electrocardiography(ECG),hematoxylin and eosin staining,and expression of glycogen phosphorylase BB(GPBB)and heart-type fatty acid-binding protein(H-FABP)were used to assess cardiac function.The effects of adrenocorticotropic hormone(ACTH)and CORT were evaluated by enzymelinked immunosorbent assay.Protein expression in the Sham and Model groups were screened by tandem mass tag-based quantitative proteomics analysis.Protein expression was evaluated by Western blotting(vimentin and GFAP)and immunofluorescence staining(GFAP).RESULTS:Compared with the Sham group,the hemorheology indicators,heart rate,ECG-ST segment elevation,and GPBB and H-FABP levels were higher in Model rats.The EA group showed reductions in these indicators compared with the Model group.Similarly,in Model rats,the expression of ACTH and CORT were significantly increased compared with the Sham group.The EA group also showed reduced expression of ACTH and CORT.Importantly,proteomics analysis showed that vimentin was differentially expressed in Model rats.Compared with the Sham group,vimentin and GFAP expression in the hippocampus was increased in the Model group but decreased in the AMI+EA group.Additionally,intraperitoneal injection of CORT aggravated the expression of GPBB,H-FABP and GFAP.CONCLUSIONS:Our results suggested that electroacupuncture may protect against cardiac injury induced by AMI through regulation of HPA axis hyperactivity,and that hippocampal GFAP may play an important role in the regulation.