Research results concerned （ 1 ） quantification of pH value and contents of ammonia nitrogen, volatile fatty acids （VFAs）, and lactic acid, which were indicators of fermentative quality of corn stover silage and o...Research results concerned （ 1 ） quantification of pH value and contents of ammonia nitrogen, volatile fatty acids （VFAs）, and lactic acid, which were indicators of fermentative quality of corn stover silage and other crop by-product silages ; （2） introduction of methods for analyses of fiber content in feeds such as detergent and enzymatic analyses; （3） determination of digestive characteristics in the tureen by artificial digestion trials （ in vitro and in situ ） ; （ 4 ） evaluation of meat productivity and quality by feeding corn stover silage to beef cattle in feeding experiments and; （5） preparation of feed composition and feeding standards for animals in Jilin Province. These results and methods substantially contributed to stabilization of feed supply as well as an increase in the efficiency of meat production, resulting in sustainable development of livestock management in China.展开更多
A new corn stover roughage processing method, corn stover extrusion and micro-storage processing technology, was introduced from the aspects of basic working principle, production and processing process, characteristi...A new corn stover roughage processing method, corn stover extrusion and micro-storage processing technology, was introduced from the aspects of basic working principle, production and processing process, characteristics of cornstalk via extrusion and micro-storage processing. The technology was proved to be a feasible and practical processing technology of stover feed with broad prospect.展开更多
[Objectives]This study was conducted to explore the effects of three different splitting fluids on the bisection effects of bovine morulae and blastocysts.[Methods]With the help of a micromanipulator,the morulae and b...[Objectives]This study was conducted to explore the effects of three different splitting fluids on the bisection effects of bovine morulae and blastocysts.[Methods]With the help of a micromanipulator,the morulae and blastocysts that were routinely produced in the body from the 6 th to the 8 th d were divided into half-embryos in vitro to observe their development,and the half-embryos with a restored morphology and a blastocyst trophoblast were selected.[Results]The success rates of bisection of morulae in PBS+0.2 mol/L sucrose and PBS+5%PVP were significantly higher than that in PBS(P<0.05),and the values in the three liquids were 95.7%,97.2% and 50%,respectively,while the blastocyst development rates and of half-embryos and the pregnancy rates of transplantation were not significantly different(P>0.05).When blastocysts were bisected in PBS+0.2 mol/L sucrose and PBS+5% PVP,the success rates of bisection were significantly higher than that of PBS(P<0.05),and the values in the three liquids were 96.8%,95.5% and 50.0%,respectively,while the development rates of half-embryos and the pregnancy rates of transplantation were not significantly different(P>0.05).The development rate of half-embryos bisected from blastocysts was significantly higher than that of morulae.[Conclusions]This study improves the efficiency of bovine embryo bisection,which is beneficial to the application of embryo bisection technology in cattle breeding.展开更多
基金Supported by Special Fund for Jilin Provincial Science and Technology Development Plan(20160204015ny)Construction of Jilin Modern Agricultural Industry Research System(201637)National Beef Cattle Yak Industry Research System(CARS-38)
文摘Research results concerned （ 1 ） quantification of pH value and contents of ammonia nitrogen, volatile fatty acids （VFAs）, and lactic acid, which were indicators of fermentative quality of corn stover silage and other crop by-product silages ; （2） introduction of methods for analyses of fiber content in feeds such as detergent and enzymatic analyses; （3） determination of digestive characteristics in the tureen by artificial digestion trials （ in vitro and in situ ） ; （ 4 ） evaluation of meat productivity and quality by feeding corn stover silage to beef cattle in feeding experiments and; （5） preparation of feed composition and feeding standards for animals in Jilin Province. These results and methods substantially contributed to stabilization of feed supply as well as an increase in the efficiency of meat production, resulting in sustainable development of livestock management in China.
基金Science and Technology Development Program of Jilin Province(20160204015ny)Construction of Jilin Modern Agricultural Industry Research System(201637)National Beef Cattle Yak Industry Research System(CARS-38)
文摘A new corn stover roughage processing method, corn stover extrusion and micro-storage processing technology, was introduced from the aspects of basic working principle, production and processing process, characteristics of cornstalk via extrusion and micro-storage processing. The technology was proved to be a feasible and practical processing technology of stover feed with broad prospect.
基金Supported by General Directive Project of Qiqihar City(NYGG-201904)National Beef and Yak Industry Reseach System Construction Project(CARS-37).
文摘[Objectives]This study was conducted to explore the effects of three different splitting fluids on the bisection effects of bovine morulae and blastocysts.[Methods]With the help of a micromanipulator,the morulae and blastocysts that were routinely produced in the body from the 6 th to the 8 th d were divided into half-embryos in vitro to observe their development,and the half-embryos with a restored morphology and a blastocyst trophoblast were selected.[Results]The success rates of bisection of morulae in PBS+0.2 mol/L sucrose and PBS+5%PVP were significantly higher than that in PBS(P<0.05),and the values in the three liquids were 95.7%,97.2% and 50%,respectively,while the blastocyst development rates and of half-embryos and the pregnancy rates of transplantation were not significantly different(P>0.05).When blastocysts were bisected in PBS+0.2 mol/L sucrose and PBS+5% PVP,the success rates of bisection were significantly higher than that of PBS(P<0.05),and the values in the three liquids were 96.8%,95.5% and 50.0%,respectively,while the development rates of half-embryos and the pregnancy rates of transplantation were not significantly different(P>0.05).The development rate of half-embryos bisected from blastocysts was significantly higher than that of morulae.[Conclusions]This study improves the efficiency of bovine embryo bisection,which is beneficial to the application of embryo bisection technology in cattle breeding.
