The effect of Xuebijing on burn patients and its effect on pain and wound healing

Objective:To explore the therapeutic effect of Xuebijing on burn patients and its effect on pain and wound healing.Methods:The burn patients treated in our hospital were selected and divided into two groups according to single and double numbers.The first group,the conventional treatment group(CT group):take conventional treatment to treat this group of burn patients;the second group,Xuebijing group(BWN group):take the CT group+Xuebijing combination treatment of this group of burn patients,37 cases in each group.There was no difference in general data between CT group and BWN group.To explore the differences between prothrombin time(PT),thrombin time(TT),fibrinogen(FIB),pain,secondary effects,and wound healing in CT group and BWN group The therapeutic effect of the patient and its effect on pain and wound healing.Results:Examination showed that the coagulation indexes of patients in CT group were 26.34±4.25,17.69±4.12,2.01±0.78,the coagulation indexes of patients in BWN group were 17.49±3.56,11.07±2.39,3.56±1.14,compared with CT group and BWN group In the CT group,the prothrombin time(PT)and thrombin time(TT)indicators were higher than those in the BWN group,but fibrinogen(FIB)was significantly lower than that in the BWN group(p<0.05).It can be seen from the table that before treatment,the visual analogue scoring(VAS)scores of patients in CT group and BWN group were 6.64±1.12 and 6.98±1.08,respectively,with a small difference(p>0.05).After treatment,CT group and BWN group scores They were 4.56±1.16 and 2.12±0.97 respectively.Compared with CT group and BWN group,the VAS score of CT group was significantly higher than that of BWN group(p<0.05).After treatment,the number of wound healing days in the BWN group(20.68±3.14d)was less than that in the CT group(32.89±4.25d)(P<0.05),but the wound healing rate(96.48±6.89%)was significantly higher than the CT group(81.17±5.74%)(P<0.05).In the CT group,3 patients had blood accumulation,2 infections,and 3 effusions.In the BWN group,there were 0 blood accumulations,1 infection,and 2 effusions.Compared with the CT group and the BWN group,the number and incidence of the BWN group(8.12%)Are less than CT group(21.62%)(p<0.05).The patient's leg suffered third-degree burns.After 5 weeks of treatment,the edge of the allogeneic skin on the CT group began to peel off and the epidermis fell off.Rejection.Conclusion:Conventional treatment+Xuebijing treatment,its curative effect is significant,can significantly accelerate the wound healing rate,and at the same time,reduce the incidence of secondary effects.

Cadaveric bone marrow mesenchymal stem cells:first experience treating a patient with large severe burns

Background:In January 2005,Rasulov et al.originally published"First experience in the use of bone marrow mesenchymal stem cells(MSCs)for the treatment of a patient with deep skin burns".Here,we present the first ever treated patient with cadaveric bone marrow mesenchymal stem cells(CMSCs)in the history of Medicine.Methods:A young man,who severely burned 60%of his total body surface with 30%of full-thickness burns while working with a grass trimmer that exploded,was involved in the study.MSCs were obtained from the bone marrow of a cadaver donor in a routine procurement procedure of CUCAIBA,the Province of Buenos Aires,Argentina,Ministry of Health,Transplantation Agency,cultured,expanded,and applied on the burned surfaces using a fibrin spray after early escharotomy.Results:So far,our preliminary experience and our early results have been very impressive showing an outstanding safety data as well as some impressive good results in the use of CMSCs.Conclusions:Based on al this,we think that improvements in the use of stem cells for burns might be possible in the near future and a lot of time as well as many lives could be saved by many other research teams all over the world.CMSCs will probably be a real scientific opportunity in Regenerative Medicine as well as in Transplantation.

Anterolateral thigh free flap for simultaneous reconstruction of digital extensor tendon and defect of the dorsal hand： A case report

This paper describes a new technique in the repair of the hand defect with digital extensor tendon injury. The anterolateral thigh flap with the thick femoral fascia has been used in the reconstruction of the composite defect of the dorsal hand, especially the defect of tendon. This technique requires short period of treatment and hence causes less damage to the donor site but shows a better recovery of the hand function. A favorable curative effect has been obtained in this patient.

Decreasing incidence of cutaneous chemical burns in a resource limited burn centre: is this a positive effect of modernization?

Background: Burns present a devastating injury to patients. Burns caused by chemical agents, present a worse scenario. In a resource limited country like Nigeria, readily available sources of these corrosive agents are mainly from lead-acid battery vendors and to some extent local small scale soap manufacturers who use caustic soda. We hypothesized that with the reduction in small scale soap manufacturing and increasing trend towards modernization in the use of dry cell batteries, chemical burns may be on the decline, and we sought to investigate this. Methods: The records of all acute burn patients seen at the Burns and Plastic Department of the National Orthopaedic Hospital Enugu Nigeria between January 2011 and December 2014 were retrospectively reviewed. The results were compared to similar studies carried out at the same centre. A questionnaire was administered to corrosive chemical (sulphuric and caustic soda) vendors to assess the trends in product sales and use in recent times. Results: A total of 624 acute burn cases were treated during the period;among which, 12 cases (1.9%) were chemical burns. When compared with previous studies at the centre, Chemical burn cases were recorded as the lowest rate. The median age of patients was 24 years. There were eight males and four females. Interpersonal assault was the commonest mechanism of injury with sulphuric acid suspected to be the commonest agent in 83.3% of the cases, while 16.7% of the cases were from accidental use of caustic soda. The head and neck as well as the upper limbs were the most affected (30%). Twenty-six questionnaires to lead-acid vendors were analyzed and revealed that all respondents noticed a marked downward trend in the sale of either sulphuric acid or caustic soda, and they attributed this to the ready availability of imported alternatives to locally manufactured soap or wet lead-acid batteries. Ease of use, durability and convenience of the dry cell batteries were cited as principal reasons. Conclusion: There appears to be a downward trend in the prevalence of chemical burns in our study compared to previous studies in the centre which may be due to reduced availability and access of corrosive chemicals to the general public. Further prospective multicentre studies to confirm this are recommended.

Sub-cytotoxic concentrations of ionic silver promote the proliferation of human keratinocytes by inducing the production of reactive oxygen species

Silver-containing preparations are widely used in the management of skin wounds, but the effects of silver ions on skin wound healing remain poorly understood. This study investigated the effects of silver ions （Ag^＋） on the proliferation of human skin keratinocytes （HaCaT） and the production of intracellular reactive oxygen species （ROS）. After treating HaCaT cells with Ag^＋ and/or the active oxygen scavenger N-acetyl cysteine （NAC）, cell proliferation and intracellular ROS generation were assessed using CCK-8 reagent and DCFH-DA fluorescent probe, respectively. In addition, 5-bromo-2-deoxyUridine （BrdU） incorporation assays, cell cycle flow cytometry, and proliferating cell nuclear antigen （PCNA） immunocytochemistry were conducted to further evaluate the effects of sub-cytotoxic Ag^＋ concentrations on HaCaT cells. The proliferation of HaCaT cells was promoted in the presence of 10^-6 and 10^-5s mol/L Ag^＋at 24, 48, and 72 h. Intracellular ROS generation also significantly increased for 5 60 min after exposure to Ag^＋ The number of BrdU-positive cells and the presence of PCNA in HaCaT cells increased 48 h after the addition of 10^-6 and 10^-5 mol/L Ag^＋, with 10^-5 mol/L Ag^＋ markedly increasing the cell proliferation index. These effects of sub-cytotoxic Ag^＋ concentrations were repressed by 5 mmol/L NAC. Our results suggest that sub-cytotoxic Ag^＋ concentrations promote the proliferation of human keratinocytes and might be associated with a moderate increase in intracellular ROS levels. This study provides important experimental evidence for developing novel silver-based wound agents or dressings with few or no cytotoxicity.