Probiotics are the treasure of the microbiology fields.They have been widely used in the food industry,clinical treatment,and other fields.The equivocal health-promoting effects and the unknown action mechanism were t...Probiotics are the treasure of the microbiology fields.They have been widely used in the food industry,clinical treatment,and other fields.The equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further probiotic’s developed applications.In recent years,various genome editing techniques have been developed and applied to explore the mechanisms and functional modifications of probiotics.As important genome editing tools,CRISPR-Cas systems that have opened new improvements in genome editing dedicated to probiotics.The high efficiency,flexibility,and specificity are the advantages of using CRISPR-Cas systems.Here,we summarize the classification and distribution of CRISPR-Cas systems in probiotics,as well as the editing tools developed on the basis of them.Then,we discuss the genome editing of probiotics based on CRISPR-Cas systems and the applications of the engineered probiotics through CRISPR-Cas systems.Finally,we proposed a design route for CRISPR systems that related to the genetically engineered probiotics.展开更多
β-Carotene is one of the most abundant natural pigments in foods;however,usage of β-carotene is limited because of its instability.Microencapsulation techniques are usually applied to protect microencapsulated p-car...β-Carotene is one of the most abundant natural pigments in foods;however,usage of β-carotene is limited because of its instability.Microencapsulation techniques are usually applied to protect microencapsulated p-carotene from oxidization.In this study,β-carotene was microencapsulated using different drying processes:spray-drying,spray freeze-dryi ng,coati ng,and spray granulation.The properties of morphology,particle size,water con tent,thermal characteristic,and chemical stability have been explored and compared.Scanning electron microscopy measure?ments showed that the coated powder had a dense surface surro un ded by starch and suggested that the coati ng process gave a microencapsulated powder with the smallest bulk density and the best compressibility among the prepared powders.The chemical stabilities of microcapsules were evaluated during six months of storage at different temperatures.The coated powder had the highest mass fraction of β-carotene,which indicated that the coating process was superior to the three other drying processes.展开更多
基金the National Key Research and Development Program of China under grant number 2022YFA0912201the National Natural Science Foundation of China under grant number 32270090+1 种基金the Foundation of Hubei Hongshan Laboratory under grant numbers 2021hszd013 and 2021hszd022the LongYun Program for College of Life Science and Technology,Huazhong Agricultural University.
文摘Probiotics are the treasure of the microbiology fields.They have been widely used in the food industry,clinical treatment,and other fields.The equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further probiotic’s developed applications.In recent years,various genome editing techniques have been developed and applied to explore the mechanisms and functional modifications of probiotics.As important genome editing tools,CRISPR-Cas systems that have opened new improvements in genome editing dedicated to probiotics.The high efficiency,flexibility,and specificity are the advantages of using CRISPR-Cas systems.Here,we summarize the classification and distribution of CRISPR-Cas systems in probiotics,as well as the editing tools developed on the basis of them.Then,we discuss the genome editing of probiotics based on CRISPR-Cas systems and the applications of the engineered probiotics through CRISPR-Cas systems.Finally,we proposed a design route for CRISPR systems that related to the genetically engineered probiotics.
基金Project supported by the Hubei Province Technology Innovation Project(No.2017ACA083),China
文摘β-Carotene is one of the most abundant natural pigments in foods;however,usage of β-carotene is limited because of its instability.Microencapsulation techniques are usually applied to protect microencapsulated p-carotene from oxidization.In this study,β-carotene was microencapsulated using different drying processes:spray-drying,spray freeze-dryi ng,coati ng,and spray granulation.The properties of morphology,particle size,water con tent,thermal characteristic,and chemical stability have been explored and compared.Scanning electron microscopy measure?ments showed that the coated powder had a dense surface surro un ded by starch and suggested that the coati ng process gave a microencapsulated powder with the smallest bulk density and the best compressibility among the prepared powders.The chemical stabilities of microcapsules were evaluated during six months of storage at different temperatures.The coated powder had the highest mass fraction of β-carotene,which indicated that the coating process was superior to the three other drying processes.
