Gravimetric resonant-inspired biosensors have attracted increasing attention in industrial and point-ofcare applications,enabling label-free detection of biomarkers such as DNA and antibodies.Capacitive micromachined ...Gravimetric resonant-inspired biosensors have attracted increasing attention in industrial and point-ofcare applications,enabling label-free detection of biomarkers such as DNA and antibodies.Capacitive micromachined ultrasonic transducers(CMUTs)are promising tools for developing miniaturized highperformance biosensing complementary metal–oxide–silicon(CMOS)platforms.However,their operability is limited by inefficient functionalization,aggregation,crosstalk in the buffer,and the requirement for an external high-voltage(HV)power supply.In this study,we aimed to propose a CMUTs-based resonant biosensor integrated with a CMOS front–end interface coupled with ethylene–glycol alkanethiols to detect single-stranded DNA oligonucleotides with large specificity.The topography of the functionalized surface was characterized by energy-dispersive X-ray microanalysis.Improved selectivity for onchip hybridization was demonstrated by comparing complementary and non-complementary singlestranded DNA oligonucleotides using fluorescence imaging technology.The sensor array was further characterized using a five-element lumped equivalent model.The 4 mm^(2) application-specific integrated circuit chip was designed and developed through 0.18 lm HV bipolar-CMOS-double diffused metal–oxide–silicon(DMOS)technology(BCD)to generate on-chip 20 V HV boosting and to track feedback frequency under a standard 1.8 V supply,with a total power consumption of 3.8 mW in a continuous mode.The measured results indicated a detection sensitivity of 7.943×10^(-3) lmol·L^(-1)·Hz^(-1) over a concentration range of 1 to 100 lmol·L^(-1).In conclusion,the label-free biosensing of DNA under dry conditions was successfully demonstrated using a microfabricated CMUT array with a 2 MHz frequency on CMOS electronics with an internal HV supplier.Moreover,ethylene–glycol alkanethiols successfully deposited self-assembled monolayers on aluminum electrodes,which has never been attempted thus far on CMUTs,to enhance the selectivity of bio-functionalization.The findings of this study indicate the possibility of full-on-chip DNA biosensing with CMUTs.展开更多
AIM To evaluate the effect on cardiovascular outcomes of sodium-glucose co-transporter-2(SGLT2) inhibitors in a real world setting by analyzing electronic medical records.METHODS We used Tri Net X, a global federated ...AIM To evaluate the effect on cardiovascular outcomes of sodium-glucose co-transporter-2(SGLT2) inhibitors in a real world setting by analyzing electronic medical records.METHODS We used Tri Net X, a global federated research network providing statistics on electronic health records(EHR). The analytics subset contained EHR from approximately 38 Million patients in 35 Health Care Organizations in the United States. The records of 46,909 patients who had taken SGLT2 inhibitors were compared to 189,120 patients with dipeptidyl peptidase(DPP) 4 inhibitors. We identified five potential confounding factors and built respective strata: elderly, hypertension, chronic kidney disease(CKD), and co-medication with either insulin or metformin. Cardiovascular events were countedas stroke(ICD10 code: I63) or myocardial infarction(ICD10: I21) occurring within three years after the first instance of the respective medication in the patients' records.RESULTS Of the 46909 patients with SGLT2 inhibitors in their EHR, 1667 patients(3.6%) had an ICD code for stroke or for myocardial infarction within the first three years after the first instance of the medication. In the control group, there were 10680 events of 189120 patients(5.6%), which represents a risk ratio of 0.63(95%CI: 0.60-0.66). The overall incidence of stroke or myocardial infarction in the strata with a potential confounding risk factor reached from 4.9% in patients taking metformin to 12.5% in the stratum with the highest risk(concomitant CKD). In all strata, the difference in risk of experiencing a cardiovascular event was similarly in favor of SGLT2 vs control, with Risk Ratio ranging from 0.62 to 0.81.CONCLUSION Real world data replicated the results from randomized clinical trials, confirmed the cardiovascular advantages of SGLT2 inhibitors, and showed its applicability to the US population.展开更多
A new low noise interface circuit for detecting weak current of micro-sensors is designed.By using the transimpedance amplifier to substitute the charge amplifier,the closed-loop circuit can avoid the phase error of t...A new low noise interface circuit for detecting weak current of micro-sensors is designed.By using the transimpedance amplifier to substitute the charge amplifier,the closed-loop circuit can avoid the phase error of the charge amplifier.Therefore,the phase compensation devices will be cancelled,because there is no phase transformation through the transimpedance amplifier.As well as,by using CCCII devices to implement the high value feedback resistor of the impedance amplifier,the noise of the I-V transformation devices is reduced,comparing with the passive resistor.The floating resistor is easy to be integrated into chips,making the integration of the interface circuit of the intelligent sensors increase.Through the simulation,the phase error of the charge amplifier is almost 9°at 2 kHz and it changes with the working frequency of the micro-sensors making the phase compensation not easy.The value of the floating resistor is 250 kΩ where the bias current is 50 μA.The noise of the active resistor is 0.037 fV2/Hz,comparing with the noise of the passive resistor,which is 4.14 fV2/Hz.展开更多
The unforeseen and uncertain life-threatening situation of the COVID-19 pandemic dramatically affected all areas of the human daily work schedule.This study was designed to assess the impact of the COVID-19 pandemic o...The unforeseen and uncertain life-threatening situation of the COVID-19 pandemic dramatically affected all areas of the human daily work schedule.This study was designed to assess the impact of the COVID-19 pandemic on blood transfusion services and discuss the adopted confrontation measures for uninterrupted blood supply during the pandemic situation.The data on blood donation,blood component preparation,and issue from January 2019 to December 2022 were collected from the inventory registers of the RBTC,Delhi,India.Compared to the non-pandemic year 2019,during the year 2020,all variables decreased gradually.The observed maximum decrease in variables such as blood collection(–79.16%)in the month of October,blood issue(–71.61%)in the month of August,random donor platelets(RDP)preparation(–98.09%)in the month of October,RDP issue(–86.08%)in the month of September,fresh frozen plasma(FFP)preparation(–100%)in the month of October,and FFP issue(–96.08%)in the month of July with an annual decrease of–45.52%,–42.87%,–33.00%,–59.79%,–40.98%,and–54.48%,respectively,as compared to year 2019.Compared to year 2020,in year 2021,the annual increase in blood collection,blood issue,FFP preparation,FFP issue,RDP preparation,and RDP issue was+50.20%,+21.68%,+65.31%,+78.52%,+116.23%,and+213.30%,respectively.Our study results show that the COVID-19 pandemic has significantly affected blood transfusion services at our blood bank.The adopted coping strategies to maintain the safe and uninterrupted blood transfusion chain at our blood bank gave us lessons for future preparedness if faced with a similar situation.展开更多
基金supported by the National Key Research and Development Program of China(2022YFB3205400)the National Natural Science Foundation of China(52275570)+1 种基金the Postdoctoral Innovation Talents Support Program(BX20230288)the Postdoctoral Science Foundation of Shaanxi Province(2018BSHEDZZ08).
文摘Gravimetric resonant-inspired biosensors have attracted increasing attention in industrial and point-ofcare applications,enabling label-free detection of biomarkers such as DNA and antibodies.Capacitive micromachined ultrasonic transducers(CMUTs)are promising tools for developing miniaturized highperformance biosensing complementary metal–oxide–silicon(CMOS)platforms.However,their operability is limited by inefficient functionalization,aggregation,crosstalk in the buffer,and the requirement for an external high-voltage(HV)power supply.In this study,we aimed to propose a CMUTs-based resonant biosensor integrated with a CMOS front–end interface coupled with ethylene–glycol alkanethiols to detect single-stranded DNA oligonucleotides with large specificity.The topography of the functionalized surface was characterized by energy-dispersive X-ray microanalysis.Improved selectivity for onchip hybridization was demonstrated by comparing complementary and non-complementary singlestranded DNA oligonucleotides using fluorescence imaging technology.The sensor array was further characterized using a five-element lumped equivalent model.The 4 mm^(2) application-specific integrated circuit chip was designed and developed through 0.18 lm HV bipolar-CMOS-double diffused metal–oxide–silicon(DMOS)technology(BCD)to generate on-chip 20 V HV boosting and to track feedback frequency under a standard 1.8 V supply,with a total power consumption of 3.8 mW in a continuous mode.The measured results indicated a detection sensitivity of 7.943×10^(-3) lmol·L^(-1)·Hz^(-1) over a concentration range of 1 to 100 lmol·L^(-1).In conclusion,the label-free biosensing of DNA under dry conditions was successfully demonstrated using a microfabricated CMUT array with a 2 MHz frequency on CMOS electronics with an internal HV supplier.Moreover,ethylene–glycol alkanethiols successfully deposited self-assembled monolayers on aluminum electrodes,which has never been attempted thus far on CMUTs,to enhance the selectivity of bio-functionalization.The findings of this study indicate the possibility of full-on-chip DNA biosensing with CMUTs.
文摘AIM To evaluate the effect on cardiovascular outcomes of sodium-glucose co-transporter-2(SGLT2) inhibitors in a real world setting by analyzing electronic medical records.METHODS We used Tri Net X, a global federated research network providing statistics on electronic health records(EHR). The analytics subset contained EHR from approximately 38 Million patients in 35 Health Care Organizations in the United States. The records of 46,909 patients who had taken SGLT2 inhibitors were compared to 189,120 patients with dipeptidyl peptidase(DPP) 4 inhibitors. We identified five potential confounding factors and built respective strata: elderly, hypertension, chronic kidney disease(CKD), and co-medication with either insulin or metformin. Cardiovascular events were countedas stroke(ICD10 code: I63) or myocardial infarction(ICD10: I21) occurring within three years after the first instance of the respective medication in the patients' records.RESULTS Of the 46909 patients with SGLT2 inhibitors in their EHR, 1667 patients(3.6%) had an ICD code for stroke or for myocardial infarction within the first three years after the first instance of the medication. In the control group, there were 10680 events of 189120 patients(5.6%), which represents a risk ratio of 0.63(95%CI: 0.60-0.66). The overall incidence of stroke or myocardial infarction in the strata with a potential confounding risk factor reached from 4.9% in patients taking metformin to 12.5% in the stratum with the highest risk(concomitant CKD). In all strata, the difference in risk of experiencing a cardiovascular event was similarly in favor of SGLT2 vs control, with Risk Ratio ranging from 0.62 to 0.81.CONCLUSION Real world data replicated the results from randomized clinical trials, confirmed the cardiovascular advantages of SGLT2 inhibitors, and showed its applicability to the US population.
基金Sponsored by the National High Technology Research Development Plan of China(Grant No.2008AA042201)
文摘A new low noise interface circuit for detecting weak current of micro-sensors is designed.By using the transimpedance amplifier to substitute the charge amplifier,the closed-loop circuit can avoid the phase error of the charge amplifier.Therefore,the phase compensation devices will be cancelled,because there is no phase transformation through the transimpedance amplifier.As well as,by using CCCII devices to implement the high value feedback resistor of the impedance amplifier,the noise of the I-V transformation devices is reduced,comparing with the passive resistor.The floating resistor is easy to be integrated into chips,making the integration of the interface circuit of the intelligent sensors increase.Through the simulation,the phase error of the charge amplifier is almost 9°at 2 kHz and it changes with the working frequency of the micro-sensors making the phase compensation not easy.The value of the floating resistor is 250 kΩ where the bias current is 50 μA.The noise of the active resistor is 0.037 fV2/Hz,comparing with the noise of the passive resistor,which is 4.14 fV2/Hz.
文摘The unforeseen and uncertain life-threatening situation of the COVID-19 pandemic dramatically affected all areas of the human daily work schedule.This study was designed to assess the impact of the COVID-19 pandemic on blood transfusion services and discuss the adopted confrontation measures for uninterrupted blood supply during the pandemic situation.The data on blood donation,blood component preparation,and issue from January 2019 to December 2022 were collected from the inventory registers of the RBTC,Delhi,India.Compared to the non-pandemic year 2019,during the year 2020,all variables decreased gradually.The observed maximum decrease in variables such as blood collection(–79.16%)in the month of October,blood issue(–71.61%)in the month of August,random donor platelets(RDP)preparation(–98.09%)in the month of October,RDP issue(–86.08%)in the month of September,fresh frozen plasma(FFP)preparation(–100%)in the month of October,and FFP issue(–96.08%)in the month of July with an annual decrease of–45.52%,–42.87%,–33.00%,–59.79%,–40.98%,and–54.48%,respectively,as compared to year 2019.Compared to year 2020,in year 2021,the annual increase in blood collection,blood issue,FFP preparation,FFP issue,RDP preparation,and RDP issue was+50.20%,+21.68%,+65.31%,+78.52%,+116.23%,and+213.30%,respectively.Our study results show that the COVID-19 pandemic has significantly affected blood transfusion services at our blood bank.The adopted coping strategies to maintain the safe and uninterrupted blood transfusion chain at our blood bank gave us lessons for future preparedness if faced with a similar situation.
