High-throughput single-cell analysis is a challenging task.Label-free tomographic phase microscopy is an excellent candidate to perform this task.However,in-line tomography is very difficult to implement in practice b...High-throughput single-cell analysis is a challenging task.Label-free tomographic phase microscopy is an excellent candidate to perform this task.However,in-line tomography is very difficult to implement in practice because it requires a complex set-up for rotating the sample and examining the cell along several directions.We demonstrate that by exploiting the random rolling of cells while they are flowing along a microfluidic channel,it is possible to obtain in-line phase-contrast tomography,if smart strategies for wavefront analysis are adopted.In fact,surprisingly,a priori knowledge of the three-dimensional position and orientation of rotating cells is no longer needed because this information can be completely retrieved through digital holography wavefront numerical analysis.This approach makes continuous-flow cytotomography suitable for practical operation in real-world,single-cell analysis and with a substantial simplification of the optical system;that is,no mechanical scanning or multi-direction probing is required.A demonstration is given for two completely different classes of biosamples:red blood cells and diatom algae.An accurate characterization of both types of cells is reported,despite their very different nature and material content,thus showing that the proposed method can be extended by adopting two alternate strategies of wavefront analysis to many classes of cells.展开更多
Melanoma is a highly aggressive tumor and almost always fatal when metastatic.Herein,we discuss recent findings on the mechanisms of resistance of human cutaneous melanoma.To achieve a precision medicine approach,the ...Melanoma is a highly aggressive tumor and almost always fatal when metastatic.Herein,we discuss recent findings on the mechanisms of resistance of human cutaneous melanoma.To achieve a precision medicine approach,the heterogeneity and plasticity of tumor cells are two crucial aspects to be investigated in depth.In fact,to understand the mechanisms that cells use to acquire a resistant phenotype after chemotherapy or how resistant cells inside the tumor are selected,it is the most important issue for a successful therapy.Since new therapeutic strategies are trying to go in this direction,we discuss here the state of the art of the research and the clinical impact of these strategies.We also discuss and suggest future research directions to develop approaches able to define the best concentration and time of exposure of the drug or the cocktails of drugs for each specific patient based on his/her biological features.展开更多
文摘High-throughput single-cell analysis is a challenging task.Label-free tomographic phase microscopy is an excellent candidate to perform this task.However,in-line tomography is very difficult to implement in practice because it requires a complex set-up for rotating the sample and examining the cell along several directions.We demonstrate that by exploiting the random rolling of cells while they are flowing along a microfluidic channel,it is possible to obtain in-line phase-contrast tomography,if smart strategies for wavefront analysis are adopted.In fact,surprisingly,a priori knowledge of the three-dimensional position and orientation of rotating cells is no longer needed because this information can be completely retrieved through digital holography wavefront numerical analysis.This approach makes continuous-flow cytotomography suitable for practical operation in real-world,single-cell analysis and with a substantial simplification of the optical system;that is,no mechanical scanning or multi-direction probing is required.A demonstration is given for two completely different classes of biosamples:red blood cells and diatom algae.An accurate characterization of both types of cells is reported,despite their very different nature and material content,thus showing that the proposed method can be extended by adopting two alternate strategies of wavefront analysis to many classes of cells.
基金The project was supported by funded from the Center for Complexity and Biosystems of UniMIThe research leading to these results has received funding from AIRC under IG 2019-ID.23141 project-P.I.La Porta Caterina.
文摘Melanoma is a highly aggressive tumor and almost always fatal when metastatic.Herein,we discuss recent findings on the mechanisms of resistance of human cutaneous melanoma.To achieve a precision medicine approach,the heterogeneity and plasticity of tumor cells are two crucial aspects to be investigated in depth.In fact,to understand the mechanisms that cells use to acquire a resistant phenotype after chemotherapy or how resistant cells inside the tumor are selected,it is the most important issue for a successful therapy.Since new therapeutic strategies are trying to go in this direction,we discuss here the state of the art of the research and the clinical impact of these strategies.We also discuss and suggest future research directions to develop approaches able to define the best concentration and time of exposure of the drug or the cocktails of drugs for each specific patient based on his/her biological features.
