Identifying candidate genes and patterns of heat-stress response in rice using a genome-wide association study and transcriptome analyses

Because high temperatures impair rice production,it is desirable to elucidate the regulatory mechanisms involved in rice response to heat stress.The objectives of this study were to identify candidate genes and characterize their response patterns during rice adaptation to high temperatures at the seedling stage.Ten heat-associated quantitative-trait loci were identified in a genome-wide association study.Comparison of transcript abundances in heat-sensitive and heat-tolerant rice pools under heat stress revealed approximately 400 differentially expressed genes.The expression of genes from heatsensitive accessions changed more than those from heat-tolerant accessions under heat stress.Alternative splicing(AS)events responded to heat stress in rice.The types of AS variants significant different between the heat-sensitive and heat-tolerant accessions.Expression patterns differing between the heat-sensitive and heat-tolerant accessions were identified for genes known to be involved in heat stress.We identified eleven genes associated with rice heat stress response.These genes could be pyramided to breed heat-tolerant rice accessions.

Rice planthopper resistance of interspecific protop last fusin line"pf9279" between O.sative and O.officinalis

An interspecific hybrid line pf9279 was obtained by proto-plast fusion between 02428(japonica, with a wide com-patibility gene) and CNW240 (O. officinalis, fromMalaysia) at CNRRI in 1992. Possible introgression of

QTLs analysis of rice peduncle vascular bundle and panicle traits

The vascular bundle in plants plays an important role intransportation of photosynthetic products, mineral nutri-ents, water, arid so on. Significant positive correlationswere found between grain yield, panicle traits and the No.of peduncle vascular bundles. So, it is very important tostudy the inheritance of peduncle vascular bundle, whichis a quantitative trait.

QTLs for ovicidal response to whitebacked planthopper in rice

Japanese japonica rice commonlyprovides with an ovicidal resistanceagainst the whitebacked planthopper(WBPH). Similarly, ovicidal activi-ty was also found to play a significantrole in a stable field resistance toWBPH in some Chinese japonicarice. Rice varieties with ovicidal re-

Effect on the application of large granule urea to irrigated rice

The effects of large granule urea(LOU) to irrigated rice were studied at the farm of China National Rice Research Institute. Experiment soil contained organic C 19.0g/kg, total N 2.2g/kg, total P 0.60g/kg, total K 20.1g/kg and pH6.9.

The recent development of rice prodaction at cold region

Located in the cold region (53 ° 29′ N) in Heihe of Heilongjiang Province, Mohe County is the northern most part in China. The county, with the duration for rice growth limited to only 100-120 d and the cumulative temperature ( over 10℃ ) reaching only around 2000 ℃, had been regarded as a ""restricted zone"" for rice production.

Pathogenic reaction of some introduced rice cultivars （lines） to seven pathotypes of bacterial blight in Hangzhou

Bacterial blight (BB) caused by Xanthomonas oryzae pv.oryzae (Xoo) is a major rice disease in China. 138 introduced cultivars (lines) were tested on pathogenicity with seven pathotypes of BB at CNRRI Experiment Station during Apt-Oct,1991.

Feasibility of fish culture under the circumstances of growing the rice on the natural water

We studied the feasibility of a new production mode incorporating rice cultivation with fish culture in 1991.

The International workshop on anther culture for rice breeders

To transfer the rice anther culture technology from successfully employing programs to emerging programs in rice-producing countries, the International Workshop on Anther Culture for Rice Breeders was held at CNRRI, 12-24 Oct, 1992. The Sponsors were Chinese Commission on Science and Technology and Rockefeller Foundation.

Preliminary report on antifertility effects of two buprofezin formulations on rice leaf folder（Cnaphalocrocis medinalis Guenée）

Buprofezin is a new insect chitin-synthesizer inhibitor for rice plant hoppers(Homoptera) and leaf-hoppers (Homoptera) control.Sreedhara Rao(1987) reported its antifertility effects on rice leaf folder (RLF,Lepidoptera).In this study,two buprofezin formulations,namely 25% Pu-shi-ling(wp) and 35% Shi-wen-ling(wp) with teo concentrations of 125mg/kg and 62.5mg/kg each,

Early ripening mutants induced by colchicine in rice

In 1981-1983, the frequency and range of the mutants induced by colchicine were investigated in M2 and M3 of two indica-rice cultivars.Seedlings of M2 and M3 were treated with 0.05% colchicine solution at 4-5 leaf stage.

Antifeeding effect of sublethal levels of Cypermethrin and Lamdacyhalothria to rice green leafhopper Nephotettix virescens

We evaluated the antifeeding effect of sublethal doses of Cypermethrin and new insecticide Lamdacyhalothrin from the ICI Agrochemicals against rice green leafhopper (GLH). Newly-emerged (0-1 d) females were used in the experiments with five replications for each. Tested females were topically treated with appropriate acetone solutions at 0.2 μl/ female.