Seed priming,a pre-sowing technique that enhances the antioxidant/DNA repair activities during the pre-germinative metabolism,still retains empirical features.We explore for the first time the molecular dynamics of pr...Seed priming,a pre-sowing technique that enhances the antioxidant/DNA repair activities during the pre-germinative metabolism,still retains empirical features.We explore for the first time the molecular dynamics of pre-germinative metabolism in primed eggplant(Solanum melongena L.)seeds in order to identify hallmarks(expression patterns of antioxidant/DNA repair genes combined with free radical profiles)useful to discriminate between high-and lowquality lots.The hydropriming protocol hereby developed anticipated(or even rescued)germination,when applied to lots with variable quality.ROS(reactive oxygen species)raised during hydropriming and dropped after dry-back.Upregulation of antioxidant/DNA repair genes was observed during hydropriming and the subsequent imbibition.Upregulation of SmOGG1(8-oxoguanine glycosylase/lyase)gene detected in primed seeds at 2 h of imbibition appeared as a promising hallmark.On the basis of these results,the investigation was restricted within the first 2 h of imbibition,to verify whether the molecular landscape was reproducible in different lots.A complex pattern of antioxidant/DNA repair gene expression emerged,reflecting the preponderance of seed lot-specific profiles.Only the low-quality eggplant seeds subjected to hydropriming showed enhanced ROS levels,both in the dry and imbibed state,and this might be a useful signature to discriminate among lots.The plasticity of eggplant pre-germinative metabolism stimulated by priming imposes a plethora of heterogeneous molecular responses that might delay the search for quality hallmarks.However,the information hereby gained could be translated to eggplant wild relatives to speed-up their use in breeding programs or other agronomical applications.展开更多
基金supported by CARIPLO Foundation in the frame of the WAKEAPT project(Code 2016-0723)(‘Seed Wake-up with Aptamers:a New Technology for Dormancy Release and Improved Seed Priming’)and by the Italian Ministry of Education,University and Research(MIUR):Dipartimenti di Eccellenza Program(2018-2022)-Dept.of Biology and Biotechnology“L.Spallanzani”,University of Pavia(to C.F.,A.P.,A.M.,and A.B.).C.F.,L.B.,and A.P.have been awarded by a Research Fellowship from CARIPLO Foundation in the frame of the WAKE-APT project(Code 2016-0723).
文摘Seed priming,a pre-sowing technique that enhances the antioxidant/DNA repair activities during the pre-germinative metabolism,still retains empirical features.We explore for the first time the molecular dynamics of pre-germinative metabolism in primed eggplant(Solanum melongena L.)seeds in order to identify hallmarks(expression patterns of antioxidant/DNA repair genes combined with free radical profiles)useful to discriminate between high-and lowquality lots.The hydropriming protocol hereby developed anticipated(or even rescued)germination,when applied to lots with variable quality.ROS(reactive oxygen species)raised during hydropriming and dropped after dry-back.Upregulation of antioxidant/DNA repair genes was observed during hydropriming and the subsequent imbibition.Upregulation of SmOGG1(8-oxoguanine glycosylase/lyase)gene detected in primed seeds at 2 h of imbibition appeared as a promising hallmark.On the basis of these results,the investigation was restricted within the first 2 h of imbibition,to verify whether the molecular landscape was reproducible in different lots.A complex pattern of antioxidant/DNA repair gene expression emerged,reflecting the preponderance of seed lot-specific profiles.Only the low-quality eggplant seeds subjected to hydropriming showed enhanced ROS levels,both in the dry and imbibed state,and this might be a useful signature to discriminate among lots.The plasticity of eggplant pre-germinative metabolism stimulated by priming imposes a plethora of heterogeneous molecular responses that might delay the search for quality hallmarks.However,the information hereby gained could be translated to eggplant wild relatives to speed-up their use in breeding programs or other agronomical applications.
