Dear Editor,The Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)and CRISPR-associated protein 9(Cas9)(CRISPR/Cas9)-mediated generation of somatically genetically engineered mouse models have emerged a...Dear Editor,The Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)and CRISPR-associated protein 9(Cas9)(CRISPR/Cas9)-mediated generation of somatically genetically engineered mouse models have emerged as a new approach for in vivo modeling of cancer[1].Here,we describe a novel DNA-free,easy,rapid,flexible,multiplexable,and robust method to model endometrial neoplasia by CRISPR/Cas9 ribonucleoprotein(RNP)electroporation into the uterus of mice.展开更多
基金supported by the Spanish Ministry of Science,Innovation and Universities(grant code PID2019-104734RB-I00)and the Spanish Association Against Cancer(grant code LABAE19004LLOB).This work was also funded by the Institute of Health Carlos III(MS17/00063)(co-founded by the European Social Fund[ESF]“investing in your future”).
文摘Dear Editor,The Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)and CRISPR-associated protein 9(Cas9)(CRISPR/Cas9)-mediated generation of somatically genetically engineered mouse models have emerged as a new approach for in vivo modeling of cancer[1].Here,we describe a novel DNA-free,easy,rapid,flexible,multiplexable,and robust method to model endometrial neoplasia by CRISPR/Cas9 ribonucleoprotein(RNP)electroporation into the uterus of mice.
