Bai Hu Ren Shen Soup promotes autophagy in the pancreas tissue of diabetic rats by increasing the level of autophagy protein LC3 and decreasing the level of autophagy protein p62

Objective:To investigate the therapeutic effect of Bai Hu Ren Shen Soup(BHRSS)on type 2 diabetes mellitus(T2DM)rats and explore whether its therapeutic effect is achieved by promoting autophagy in the pancreas tissue of T2DM rats.Methods:Thirty male standard deviation rats were equally divided into normal control group,T2DM group and BHRSS group.Firstly,the therapeutic effect of BHRSS on T2DM was evaluated by biochemical indices in serum and pathological changes in the pancreas tissue of rats in each group after modeling and medication.Then,the levels of microtubule-associated protein 1 light chain 3(LC3)protein and p62 protein in the pancreas tissue of rats in each group after BHRSS intervention were detected by Western blot.Results:BHRSS intervention significantly reduced the levels of blood sugar,liver and kidney function,and other related biochemical indices in the serum of T2DM rats,and effectively improved the pathological changes in the pancreas tissue of T2DM rats.In addition,Western blot results showed that the expression levels of autophagy-related protein LC3 protein in the pancreas tissue of rats were increased,and the expression level of p62 protein was decreased after BHRSS intervention.Conclusion:BHRSS has a clear therapeutic effect on T2DM model rats,which may be related to that increase of autophagy protein LC3 and the decrease of p62 protein level in the pancreas tissue.

The anti-hypertensive effect of time acupuncture on the patients with primary hypertension

Objective:To observe the anti-hypertensive effect of time acupuncture on primary hypertension so as to provide the therapeutic regimen for the treatment of primary hypertension.Methods:A total of 120 patients with primary hypertension were randomized into a western medication group and an acupuncture-medication group,60 cases in each one.In the western medication group,levoamlodipine maleate tablets were prescribed for oral administration.In the acupuncture-medication group,on the base of the treatment as the western medication group,time acupuncture was combined in the period of a day from 7:00 a.m.to 9:00 a.m.The duration of treatment was 4 weeks in the two groups.Before and after treatment,blood pressure,serum endothelin(ET)and nitrious oxide(NO)were measured successively and the clinical therapeutic effect was evaluated.Results:(1)Blood pressure:the systolic and diastolic pressure after treatment were all lower than that before treatment in the two groups,indicating statistical differences(all P<0.05),After treatment,the systolic and diastolic pressure in the acupuncture-medication group were lower than that of the western medication group,indicating significant differences(both P<0.05).(2)ET and NO:after treatment,ET in the acupuncture-medication group was lower than the western medication group and NO was higher than the western medication group,indicating statistical differences(both P<0.05).(3)Clinical therapeutic effect:the total effective effect of anti-hypertension was 89%in the acupuncture-medication group,higher than 63%in the western medication group,indicating statistical difference(P<0.05).Conclusion:The combined treatment of western medication and time acupuncture(7:00 am to 9:00 am)achieves better effect on primary hypertension as compared with simple western medication and its anti-hypertensive effect is probably related to the decrease of ET and the increase of NO.