In agricultural producer countries, the use of chlorinated herbicides (atrazine), associated to intensive farming practices, overflows the ecosystems natural attenuation capacity. Thus, herbicide residues contaminat...In agricultural producer countries, the use of chlorinated herbicides (atrazine), associated to intensive farming practices, overflows the ecosystems natural attenuation capacity. Thus, herbicide residues contaminate the soils and water sources, increasing the risk for human health and wildlife. Humid Pampa is the main agricultural region in one of the world's largest herbicide consumer countries; hence, technologies to remediate its residues are needed. In this context, phytoremediation is an effective, environmental friendly and publicly accepted option. Accordingly, the objective of designing biotechnological strategies for phytoremediating triazinic herbicide residues in an integrated approach to the ongoing Humid Pampa agricultural trend is raised. In this way, strategies according to the edaphic, topogeographic and fanning practices characteristics of this region where established. In those strategies, the adequate selection of plant species is essential. For that purpose, atrazine tolerance assays were performed in simple (semisolid agar medium) and complex (microcosms) experimental systems implanted with Lolium multiflorum, Lotus sp. and Quenopodium quinoa. L. multiflorum was selected and evaluated via atrazine dissipation assays with soil. Atrazine soil content was determined by solvent extraction and subsequent HPLC-UV analysis, observing statistically significant attenuation levels at 21 days. According to the observed results, a strategy for scaling up the application to field conditions is proposed.展开更多
文摘In agricultural producer countries, the use of chlorinated herbicides (atrazine), associated to intensive farming practices, overflows the ecosystems natural attenuation capacity. Thus, herbicide residues contaminate the soils and water sources, increasing the risk for human health and wildlife. Humid Pampa is the main agricultural region in one of the world's largest herbicide consumer countries; hence, technologies to remediate its residues are needed. In this context, phytoremediation is an effective, environmental friendly and publicly accepted option. Accordingly, the objective of designing biotechnological strategies for phytoremediating triazinic herbicide residues in an integrated approach to the ongoing Humid Pampa agricultural trend is raised. In this way, strategies according to the edaphic, topogeographic and fanning practices characteristics of this region where established. In those strategies, the adequate selection of plant species is essential. For that purpose, atrazine tolerance assays were performed in simple (semisolid agar medium) and complex (microcosms) experimental systems implanted with Lolium multiflorum, Lotus sp. and Quenopodium quinoa. L. multiflorum was selected and evaluated via atrazine dissipation assays with soil. Atrazine soil content was determined by solvent extraction and subsequent HPLC-UV analysis, observing statistically significant attenuation levels at 21 days. According to the observed results, a strategy for scaling up the application to field conditions is proposed.