Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

学习的主要目标是多识别人的新奇来源有势力房间，克服涉及胚胎的干细胞研究和很成年的干细胞的有限可获得性的道德的问题。羊膜的液体房间(声频抗流圈) 习惯性地为出生前的诊断被获得并且能在 vitro 被扩展；他们的起源和性质的不过当前的知识是有限的。声频抗流圈的二十件样品在文化暴露于 adipogenic， osteogenic，神经原并且 myogenic 媒介。区别用免疫细胞化学， RT-PCR 并且西方的弄污被评估。在处理前，声频抗流圈显示出异构的形态学。他们为 MyoD， Myf-5， MRF4， Myogenin 和 Desmin 是否定的但是为由 RT-PCR 的 osteocalcin， PPARgamma2， GAP43， NSE， Nestin， MAP2， GFAP 和贝它导管素 III 积极。房间表示了 Oct-4， Rex-1 和 Runx-1，它描绘无差别的干细胞状态。由免疫细胞化学，他们表示了 neural-glial 蛋白质，间充质、上皮的标记。在文化以后，当占优势的细胞的部件是 fibroblastic 时，声频抗流圈区分了进 adipocytes 和造骨细胞。就算没有 neuronal 形态学是可检测的，早、迟了的 neuronal 抗原仍然是在在神经特定的媒介的 2 星期文化以后的现在。我们的结果提供人的羊膜的液体与为几个系显示出茎和织物特定的基因 / 蛋白质存在的多系潜力包含祖先房间的证据。

Roles of the fibroblast growth factor signal transduction system in tissue injury repair

Following injury,tissue autonomously initiates a complex repair process,resulting in either partial recovery or regeneration of tissue architecture and function in most organisms.Both the repair and regeneration processes are highly coordinated by a hierarchy of interplay among signal transduction pathways initiated by different growth factors,cytokines and other signaling molecules under normal conditions.However,under chronic traumatic or pathological conditions,the reparative or regenerative process of most tissues in different organs can lose control to different extents,leading to random,incomplete or even flawed cell and tissue reconstitution and thus often partial restoration of the original structure and function,accompanied by the development of fibrosis,scarring or even pathogenesis that could cause organ failure and death of the organism.Ample evidence suggests that the various combinatorial fibroblast growth factor(FGF)and receptor signal transduction systems play prominent roles in injury repair and the remodeling of adult tissues in addition to embryonic development and regulation of metabolic homeostasis.In this review,we attempt to provide a brief update on our current understanding of the roles,the underlying mechanisms and clinical application of FGFs in tissue injury repair.

Hypoxia response element-directed expression of bFGF in dental pulp stem cells improve the hypoxic environment by targeting pericytes in SCI rats

Cell-based transplantation strategies possess great potential for spinal cord injury(SCI)repair.Basic fibroblast growth factor(bFGF)has been reported to have multiple neuro-promoting effects on developing and adult nervous system of mammals and considered a promising therapy for nerve injury following SCI.Human dental pulp stem cells(DPSCs)are abundant stem cells with low immune rejection,which can be considered for cell replacement therapy.The purpose of this study was to investigate the roles of DPSCs which express bFGF under the regulation of five hypoxia-responsive elements(5HRE)using an adeno-associated virus(AAV-5HRE-bFGF-DPSCs)in SCI repairing model.In this study,DPSCs were revealed to differentiate into CD13^(+)pericytes and up-regulate N-cadherin expression to promote the re-attachment of CD13^(+)pericytes to vascular endothelial cells.The re-attachment of CD13^(+)pericytes to vascular endothelial cells subsequently increased the flow rate of blood in microvessels via the contraction of protuberance.As a result,increased numbers of red blood cells carried more oxygen to the damaged area and the local hypoxia microenvironment in SCI was improved.Thus,this study represents a step forward towards the potential use of AAV-5HRE-bFGF-DPSCs in SCI treatment in clinic.

Myocardial protection by heparin-based coacervate of FGF10

Heart disease is still the leading killer all around the world,and its incidence is expected to increase over the next decade.Previous reports have already shown the role of fibroblast growth factor10(FGF10)in alleviating heart diseases.However,FGF10 has not been used to treat heart diseases because the free protein has short half-life and low bioactivity.Here,an injectable coacervate was designed to protect growth factor from degradation during delivery and the effects of the FGF10 coacervate were studied using a mice acute myocardial infarction(MI)model.As shown in our echocardiographic results,a single injection of FGF10 coacervate effectively inhibited preserved cardiac contractibility and ventricular dilation when compared with free FGF10 and the saline treatment 6 weeks after MI.It is revealed in histological results that the MI induced myocardial inflammation and fibrosis was reduced after FGF10 coacervate treatment.Furthermore,FGF10 coacervate treatment could improve arterioles and capillaries stabilization through increasing the proliferation of endothelial and mural cells.However,with the same dosage,no statistically significant difference was shown between free FGF10,heparin+FGF10 and saline treatment,especially in long term.On another hand,FGF10 coacervate also increased the expression of cardiac-associated the mRNA(cTnT,Cx43 and α-SMA),angiogenic factors(Ang-1 and VEGFA)and decreased the level of inflammatory factor(tumor necrosis factor-α).The downstream signaling of the FGF10 was also investigated,with the western blot results showing that FGF10 coacervate activated the p-FGFR,PI3K/Akt and ERK1/2 pathways to a more proper level than free FGF10 or heparin+FGF10.In general,it is revealed in this research that one-time injection of FGF10 coacervate sufficiently attenuated MI induced injury when compared with an equal dose of free FGF10 or heparin+FGF10 injection.

Pollens derived magnetic porous particles for adsorption of low-density lipoprotein from plasma

Adsorption of low-density lipoprotein from plasma is vital for the treatment of dyslipidemia.Appropriate adsorbent material for efficient and selective adsorption of low-density lipoprotein is highly desired.In this work,we developed pollens-derived magnetic porous particles as adsorbents for this purpose.The natural pollen grains were modified to obtain high surface porosity,a large inner cavity,magnet responsiveness,and specific wettability.The resultant particles exhibited satisfying performance in the adsorption of a series of oils and organic solvents out of water.Besides,the particles were directly utilized to the adsorption of low-density lipoprotein in plasma,which showed high selectivity,and achieved an outstanding adsorption capacity as high as 34.9%within 2 h.Moreover,their salient biocompatibility was demonstrated through simulative hemoperfusion experiments.These features,together with its abundant source and facile fabrication,makes the pollens-derived magnetic porous particles excellent candidate for low-density lipoprotein-apheresis and water treatment applications.