Objective:To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus(E.granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion...Objective:To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus(E.granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion.Methods:Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography.Four young rabbits were selected and challenged intradermallt with yielded recombinant antigens.Rabbits’ sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last injection.Results:The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post infection.Conclusions: Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.展开更多
Research suggests that inflammation is important in the pathophysiology of mental disorders.In addition,a growing body of evidence has led to the concept of the microbiota-gut-brain axis.To understand the potential in...Research suggests that inflammation is important in the pathophysiology of mental disorders.In addition,a growing body of evidence has led to the concept of the microbiota-gut-brain axis.To understand the potential interactions,we begin by exploring the liaison between the immune system and mental disorders,then we describe the evidence that the microbiota impact the immune response in the developing brain.Next,we review the literature that has documented microbiome alterations in major mental disorders.We end with a summary of therapeutic applications,ranging from psycho-biotics to immunomodulatory drugs that could affect the microbiotagut-brain axis,and potential treatments to alleviate the adverse effects of antipsycho tics.We conclude that there is promising evidence to support the position that the microbiota plays an important role in the immunological pathophysiology of mental disorders with an emphasis on psychotic disorders and mood disorders.However,more research is needed to elucidate the mechanisms.展开更多
基金supported financially by Tehran University of Medical Sciencesimplemented in Cellular and Molecular Biology Research Center of Shahid Beheshti University of Medical Sciences,Tehran,Iran
文摘Objective:To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus(E.granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion.Methods:Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography.Four young rabbits were selected and challenged intradermallt with yielded recombinant antigens.Rabbits’ sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last injection.Results:The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post infection.Conclusions: Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.
基金supported by the National Natural Science Foundation of China (81871056)。
文摘Research suggests that inflammation is important in the pathophysiology of mental disorders.In addition,a growing body of evidence has led to the concept of the microbiota-gut-brain axis.To understand the potential interactions,we begin by exploring the liaison between the immune system and mental disorders,then we describe the evidence that the microbiota impact the immune response in the developing brain.Next,we review the literature that has documented microbiome alterations in major mental disorders.We end with a summary of therapeutic applications,ranging from psycho-biotics to immunomodulatory drugs that could affect the microbiotagut-brain axis,and potential treatments to alleviate the adverse effects of antipsycho tics.We conclude that there is promising evidence to support the position that the microbiota plays an important role in the immunological pathophysiology of mental disorders with an emphasis on psychotic disorders and mood disorders.However,more research is needed to elucidate the mechanisms.
