Objective To investigate the causes andmanagement of postsplenectomy continuous fever in portal hypertensive patients. Methods The clinical data of 329 portal hypertension patients with liver cirrhosis undergoing sple...Objective To investigate the causes andmanagement of postsplenectomy continuous fever in portal hypertensive patients. Methods The clinical data of 329 portal hypertension patients with liver cirrhosis undergoing splenectomy from 1992 to 2001 were reviewed. Among those,772 patients suffered from a fever higher than 38. 5℃ for more than 2 weeks postoperatively. Results The most common cause was portal or splenic vein thrombosis and hematocele or hydrops and infection in splenic recess. Portal or splenic vein thrombosis was related to the surgical modality adopted and liver function. Conclusion Portal system thrombosis and hematocele, hydrops, or infection in the splenic recess were among the most common causes leading to postoperative long-term fever in these patients. Active anticoagulant use, effective drainage, antibiotic therapy and judicious application of surgical modality will help to reduce the rate of postoperative fever. 4 refs.展开更多
Objective To investigate the expression of cancer-testis antigen CT10 gene mRNA in hepatocellular carcinoma and predict the HLA-A2-restricted CTL epitopes of CT10. Methods The expression of CT10 mRNA was detected by u...Objective To investigate the expression of cancer-testis antigen CT10 gene mRNA in hepatocellular carcinoma and predict the HLA-A2-restricted CTL epitopes of CT10. Methods The expression of CT10 mRNA was detected by using RT - PCR in HCC tissues and the corresponding adjacent non-HCC tissues from 45 HCC patients, among those 3 samples selected randomly from CT10 PCR positive products were sequenced. HLA - A2-restricted CTL epitopes of CT10 was predicted by peptide supermotif prediction combined with quantitative motif. Results CT10 mRNA was detectable in 19/45 (42. 2%) of HCC samples, while the corresponding adjacent non-HCC tissue were all negative in expression of CT10 mRNA. In addition, The DNA sequence confirmed that the RT- PCR products were truly CT10 cDNA. No June 2003 Vol12 No2 relationship was found between the expression of CT10 and demographic and clinical features such as age, sex, tumor size, degree of tumor differentiation, serum α-fetoprotein level and infection of hepatitis B virus or展开更多
文摘Objective To investigate the causes andmanagement of postsplenectomy continuous fever in portal hypertensive patients. Methods The clinical data of 329 portal hypertension patients with liver cirrhosis undergoing splenectomy from 1992 to 2001 were reviewed. Among those,772 patients suffered from a fever higher than 38. 5℃ for more than 2 weeks postoperatively. Results The most common cause was portal or splenic vein thrombosis and hematocele or hydrops and infection in splenic recess. Portal or splenic vein thrombosis was related to the surgical modality adopted and liver function. Conclusion Portal system thrombosis and hematocele, hydrops, or infection in the splenic recess were among the most common causes leading to postoperative long-term fever in these patients. Active anticoagulant use, effective drainage, antibiotic therapy and judicious application of surgical modality will help to reduce the rate of postoperative fever. 4 refs.
文摘Objective To investigate the expression of cancer-testis antigen CT10 gene mRNA in hepatocellular carcinoma and predict the HLA-A2-restricted CTL epitopes of CT10. Methods The expression of CT10 mRNA was detected by using RT - PCR in HCC tissues and the corresponding adjacent non-HCC tissues from 45 HCC patients, among those 3 samples selected randomly from CT10 PCR positive products were sequenced. HLA - A2-restricted CTL epitopes of CT10 was predicted by peptide supermotif prediction combined with quantitative motif. Results CT10 mRNA was detectable in 19/45 (42. 2%) of HCC samples, while the corresponding adjacent non-HCC tissue were all negative in expression of CT10 mRNA. In addition, The DNA sequence confirmed that the RT- PCR products were truly CT10 cDNA. No June 2003 Vol12 No2 relationship was found between the expression of CT10 and demographic and clinical features such as age, sex, tumor size, degree of tumor differentiation, serum α-fetoprotein level and infection of hepatitis B virus or
