The inevitable gap between in vitro and in vivo degradation rate of biomaterials has been a challenging factor in the optimal designing of scaffold’s degradation to be balanced with new tissue formation.To enable non...The inevitable gap between in vitro and in vivo degradation rate of biomaterials has been a challenging factor in the optimal designing of scaffold’s degradation to be balanced with new tissue formation.To enable non-/minimum-invasive tracking of in vivo scaffold degradation,chemical modifications have been applied to label polymers with fluorescent dyes.However,the previous approaches may have limited expandability due to complicated synthesis processes.Here,we introduce a simple and efficient method to fluorescence labeling of polymeric scaffolds via blending with near-infrared(NIR)quantum dots(QDs),semiconductor nanocrystals with superior optical properties.QDs-labeled,3D-printed PCL scaffolds showed promising efficiency and reliability in quantitative measurement of degradation using a custom-built fiber-optic imaging modality.Furthermore,QDs-PCL scaffolds showed neither cytotoxicity nor secondary labeling of adjacent cells.QDs-PCL scaffolds also supported the engineering of fibrous,cartilaginous,and osteogenic tissues from mesenchymal stem/progenitor cells(MSCs).In addition,QDs-PCL enabled a distinction between newly forming tissue and the remaining mass of scaffolds through multi-channel imaging.Thus,our findings suggest a simple and efficient QDs-labeling of PCL scaffolds and minimally invasive imaging modality that shows significant potential to enable in vivo tracking of scaffold degradation as well as new tissue formation.展开更多
The periodontium is an integrated,functional unit of multiple tissues surrounding and supporting the tooth,including but not limited to cementum(CM),periodontal ligament(PDL)and alveolar bone(AB).Periodontal tissues c...The periodontium is an integrated,functional unit of multiple tissues surrounding and supporting the tooth,including but not limited to cementum(CM),periodontal ligament(PDL)and alveolar bone(AB).Periodontal tissues can be destructed by chronic periodontal disease,which can lead to tooth loss.In support of the treatment for periodontally diseased tooth,various biomaterials have been applied starting as a contact inhibition membrane in the guided tissue regeneration(GTR)that is the current gold standard in dental clinic.Recently,various biomaterials have been prepared in a form of tissue engineering scaffold to facilitate the regeneration of damaged periodontal tissues.From a physical substrate to support healing of a single type of periodontal tissue to multi-phase/bioactive scaffold system to guide an integrated regeneration of periodontium,technologies for scaffold fabrication have emerged in last years.This review covers the recent advancements in development of scaffolds designed for periodontal tissue regeneration and their efficacy tested in vitro and in vivo.Pros and Cons of different biomaterials and design parameters implemented for periodontal tissue regeneration are also discussed,including future perspectives.展开更多
基金This study is supported by NIH Grants 1R01DE029321 to C.H.L.
文摘The inevitable gap between in vitro and in vivo degradation rate of biomaterials has been a challenging factor in the optimal designing of scaffold’s degradation to be balanced with new tissue formation.To enable non-/minimum-invasive tracking of in vivo scaffold degradation,chemical modifications have been applied to label polymers with fluorescent dyes.However,the previous approaches may have limited expandability due to complicated synthesis processes.Here,we introduce a simple and efficient method to fluorescence labeling of polymeric scaffolds via blending with near-infrared(NIR)quantum dots(QDs),semiconductor nanocrystals with superior optical properties.QDs-labeled,3D-printed PCL scaffolds showed promising efficiency and reliability in quantitative measurement of degradation using a custom-built fiber-optic imaging modality.Furthermore,QDs-PCL scaffolds showed neither cytotoxicity nor secondary labeling of adjacent cells.QDs-PCL scaffolds also supported the engineering of fibrous,cartilaginous,and osteogenic tissues from mesenchymal stem/progenitor cells(MSCs).In addition,QDs-PCL enabled a distinction between newly forming tissue and the remaining mass of scaffolds through multi-channel imaging.Thus,our findings suggest a simple and efficient QDs-labeling of PCL scaffolds and minimally invasive imaging modality that shows significant potential to enable in vivo tracking of scaffold degradation as well as new tissue formation.
基金This manuscript was in part supported by National Institutes of Health grant 1R01DE029321-01A1 to C.H.L.
文摘The periodontium is an integrated,functional unit of multiple tissues surrounding and supporting the tooth,including but not limited to cementum(CM),periodontal ligament(PDL)and alveolar bone(AB).Periodontal tissues can be destructed by chronic periodontal disease,which can lead to tooth loss.In support of the treatment for periodontally diseased tooth,various biomaterials have been applied starting as a contact inhibition membrane in the guided tissue regeneration(GTR)that is the current gold standard in dental clinic.Recently,various biomaterials have been prepared in a form of tissue engineering scaffold to facilitate the regeneration of damaged periodontal tissues.From a physical substrate to support healing of a single type of periodontal tissue to multi-phase/bioactive scaffold system to guide an integrated regeneration of periodontium,technologies for scaffold fabrication have emerged in last years.This review covers the recent advancements in development of scaffolds designed for periodontal tissue regeneration and their efficacy tested in vitro and in vivo.Pros and Cons of different biomaterials and design parameters implemented for periodontal tissue regeneration are also discussed,including future perspectives.