The flow-through hybridization and gene chip(FHGC)was developed to used in clinical molecular diagnostics for thalassemia,human papillomavirus(HPV),and other dis-eases.FHGC could improve hybridization efficiency and r...The flow-through hybridization and gene chip(FHGC)was developed to used in clinical molecular diagnostics for thalassemia,human papillomavirus(HPV),and other dis-eases.FHGC could improve hybridization efficiency and reduce hands-on time,thus improving precision,repro-ducibility,and traceability.Multiple genotypes can be detected simultaneously without incurring high costs.^(1)During the experiment,the polymerase chain reaction(PCR)product or buffer forced through the membrane matrix,PCR products are apprehended by a probe attached to the membrane.展开更多
基金supported by the National Natural Science Foundation of China(No.82060388)Science and Technology Department of Guizhou Province(No.QKHJC-ZK[2021-396],QKHJC[2020]1Y424)+2 种基金Healthcare Commission of Guizhou Province(China)(No.GZWJKJ2019-1-192,GZWJKJ2021-310)Guiyang Science and Technology Bureau Guizhou,China(No.ZKH2022-4-3)Guizhou Department and Platform Talents(China)(No.[2018]5766-1).
文摘The flow-through hybridization and gene chip(FHGC)was developed to used in clinical molecular diagnostics for thalassemia,human papillomavirus(HPV),and other dis-eases.FHGC could improve hybridization efficiency and reduce hands-on time,thus improving precision,repro-ducibility,and traceability.Multiple genotypes can be detected simultaneously without incurring high costs.^(1)During the experiment,the polymerase chain reaction(PCR)product or buffer forced through the membrane matrix,PCR products are apprehended by a probe attached to the membrane.
