Lack of suitable electron donors or acceptors is in many cases the key reason for pollutants to persist in the environment.Externally supplementation of electron donors or acceptors is often difficult to control and/o...Lack of suitable electron donors or acceptors is in many cases the key reason for pollutants to persist in the environment.Externally supplementation of electron donors or acceptors is often difficult to control and/or involves chemical additions with limited lifespan,residue formation or other adverse side effects.Microbial electrochemistry has evolved very fast in the past years–this field relates to the study of electrochemical interactions between microorganisms and solid-state electron donors or acceptors.Current can be supplied in such so-called bioelectrochemical systems(BESs)at low voltage to provide or extract electrons in a very precise manner.A plethora of metabolisms can be linked to electrical current now,from metals reductions to denitrification and dechlorination.In this perspective,we provide an overview of the emerging applications of BES and derived technologies towards the bioremediation field and outline how this approach can be game changing.展开更多
Biogenic palladium nanoparticles(bio-Pd NPs)are used for the reductive transformation and/or dehalogenation of persistent micropollutants.In this work,H2(electron donor)was produced in situ by an electrochemical cell,...Biogenic palladium nanoparticles(bio-Pd NPs)are used for the reductive transformation and/or dehalogenation of persistent micropollutants.In this work,H2(electron donor)was produced in situ by an electrochemical cell,permitting steered production of differently sized bio-Pd NPs.The catalytic activity was first assessed by the degradation of methyl orange.The NPs showing the highest catalytic activity were selected for the removal of micropollutants from secondary treated municipal wastewater.The synthesis at different H2 flow rates(0.310 L/hr or 0.646 L/hr)influenced the bio-Pd NPs size.The NPs produced over 6 hr at a lowH2 flow rate had a larger size(D50=39.0 nm)than those produced in 3 hr at a high H2 flow rate(D50=23.2 nm).Removal of 92.1%and 44.3%of methyl orange was obtained after 30 min for the NPs with sizes of 39.0 nm and 23.2 nm,respectively.Bio-Pd NPs of 39.0 nm were used to treat micropollutants present in secondary treated municipal wastewater at concentrations ranging fromμg/L to ng/L.Effective removal of 8 compounds was observed:ibuprofen(69.5%)<sulfamethoxazole(80.6%)<naproxen(81.4%)<furosemide(89.7%)<citalopram(91.7%)<diclofenac(91.9%)<atorvastatin(>94.3%)<lorazepam(97.2%).Re-moval of fluorinated antibiotics occurred at>90%efficiency.Overall,these data indicate that the size,and thus the catalytic activity of the NPs can be steered and that the removal of challengingmicropollutants at environmentally relevant concentrations can be achieved through the use of bio-Pd NPs.展开更多
Microbial electrosynthesis(MES)enables the bioproduction of multicarbon compounds from CO_(2)using electricity as the driver.Although high salinity can improve the energetic performance of bioelectrochemical systems,a...Microbial electrosynthesis(MES)enables the bioproduction of multicarbon compounds from CO_(2)using electricity as the driver.Although high salinity can improve the energetic performance of bioelectrochemical systems,acetogenic processes under elevated salinity are poorly known.Here MES under 35e60 g L^(-1)salinity was evaluated.Acetate production in two-chamber MES systems at 35 g L^(-1)salinity(seawater composition)gradually decreased within 60 days,both under-1.2 V cathode potential(vs.Ag/AgCl)and^(-1).56 A m^(-2)reductive current.Carbonate precipitation on cathodes(mostly CaCO3)likely declined the production through inhibiting CO_(2)supply,the direct electrode contact for acetogens and H2 production.Upon decreasing Ca2t and Mg2t levels in three-chamber reactors,acetate was stably produced over 137 days along with a low cathode apparent resistance at 1.9±0.6 mU m^(2)and an average production rate at 3.80±0.21 g m^(-2)d^(-1).Increasing the salinity step-wise from 35 to 60 g L^(-1)gave the most efficient acetate production at 40 g L^(-1)salinity with average rates of acetate production and CO_(2)consumption at 4.56±3.09 and 7.02±4.75 g m^(-2)d^(-1),respectively.The instantaneous coulombic efficiency for VFA averaged 55.1±31.4%.Acetate production dropped at higher salinity likely due to the inhibited CO_(2)dissolution and acetogenic metabolism.Acetobacterium up to 78%was enriched on cathodes as the main acetogen at 35 g L^(-1).Under high-salinity selection,96.5%Acetobacterium dominated on the cathode along with 34.0%Sphaerochaeta in catholyte.This research provides a first proof of concept that MES starting from CO_(2)reduction can be achieved at elevated salinity.展开更多
Seasonal or permanent water scarcity in off-grid communities can be alleviated by recycling water in decentralized wastewater treatment systems.Nature-based solutions,such as constructed wetlands(CWs),have become popu...Seasonal or permanent water scarcity in off-grid communities can be alleviated by recycling water in decentralized wastewater treatment systems.Nature-based solutions,such as constructed wetlands(CWs),have become popular solutions for sanitation in remote locations.Although typical CWs can efficiently remove solids and organics to meet water reuse standards,polishing remains necessary for other parameters,such as pathogens,nutrients,and recalcitrant pollutants.Different CW designs and CWs coupled with electrochemical technologies have been proposed to improve treatment efficiency.Electrochemical systems(ECs)have been either implemented within the CW bed(ECin-CW)or as a stage in a sequential treatment(CW+EC).A large body of literature has focused on ECin-CW,and multiple scaled-up systems have recently been successfully implemented,primarily to remove recalcitrant organics.Conversely,only a few reports have explored the opportunity to polish CW effluents in a downstream electrochemical module for the electro-oxidation of micropollutants or electro-disinfection of pathogens to meet more stringent water reuse standards.This paper aims to critically review the opportunities,challenges,and future research directions of the different couplings of CW with EC as a decentralized technology for water treatment and recovery.展开更多
The anaerobic digestion process has been one of the key processes for renewable energy recovery from organic waste streams for over a century.The anaerobic digestion microbiome is,through the continuous development of...The anaerobic digestion process has been one of the key processes for renewable energy recovery from organic waste streams for over a century.The anaerobic digestion microbiome is,through the continuous development of novel techniques,evolving from a black box to a well-defined consortium,but we are not there yet.In this perspective,I provide my view on the current status and challenges of the anaerobic digestion microbiome,as well as the opportunities and solutions to exploit it.I consider identification and fingerprinting of the anaerobic digestion microbiome as complementary tools to monitor the anaerobic digestion microbiome.However,data availability,method-inherent biases and correct taxa identification hamper the accuracy and reproducibility of anaerobic digestion microbiome characterization.Standardisation of microbiome research in anaerobic digestion and other engineered systems will be essential in the coming decades,for which I proposed some targeted solutions.These will bring anaerobic digestion from a single-purpose energy-recovery technology to a versatile process for integrated resource recovery.It is my opinion that the exploitation of the microbiome will be a driver of innovation,and that it has a key role to play in the bio-based economy of the decades to come.展开更多
are time-consuming and not sensitive enough.However,bacteria typically connect to electrodes through biofilm formation,leading to problems due to lack of uniformity or long device production times.A suitable immobilis...are time-consuming and not sensitive enough.However,bacteria typically connect to electrodes through biofilm formation,leading to problems due to lack of uniformity or long device production times.A suitable immobilisation technique can overcome these challenges.Still,they may respond more slowly than biofilm-based electrodes because bacteria gradually adapt to electron transfer during biofilm formation.In this study,we propose a controlled and reproducible way to fabricate bacteria-modified electrodes.The method consists of an immobilisation step using a cellulose matrix,followed by an electrode polarization in the presence of ferricyanide and glucose.Our process is short,reproducible and led us to obtain ready-to-use electrodes featuring a high-current response.An excellent shelf-life of the immobilised electrochemically active bacteria was demonstrated for up to one year.After an initial 50% activity loss in the first month,no further declines have been observed over the following 11 months.We implemented our bacteria-modified electrodes to fabricate a lateral flow platform for toxicity monitoring using formaldehyde(3%).Its addition led to a 59% current decrease approximately 20 min after the toxic input.The methods presented here offer the ability to develop a high sensitivity,easy to produce,and long shelf life bacteria-based toxicity detectors.展开更多
基金funded through the European Union’s Horizon 2020 project ELECTRA under grant agreement No.826244National Natural Science Foundation of China(NSFC)(No.31861133001,31861133002,31861133003)+3 种基金Serra Hunter Fellow(UdG-AG-575)the funding from the ICREA Academia awarthe Catalan Government with code 2017-SGR-1552supported by the Ghent University special research fund under grant No.BOF19/GOA/026.
文摘Lack of suitable electron donors or acceptors is in many cases the key reason for pollutants to persist in the environment.Externally supplementation of electron donors or acceptors is often difficult to control and/or involves chemical additions with limited lifespan,residue formation or other adverse side effects.Microbial electrochemistry has evolved very fast in the past years–this field relates to the study of electrochemical interactions between microorganisms and solid-state electron donors or acceptors.Current can be supplied in such so-called bioelectrochemical systems(BESs)at low voltage to provide or extract electrons in a very precise manner.A plethora of metabolisms can be linked to electrical current now,from metals reductions to denitrification and dechlorination.In this perspective,we provide an overview of the emerging applications of BES and derived technologies towards the bioremediation field and outline how this approach can be game changing.
基金The ELECTRA project has received funding from European Union’s Horizon 2020 research and innovation programunder grant agreement No.826244Eduardo Bolea-Fernandez thanks FWO-Vlaanderen for his postdoctoral grant(No.12ZA320N),The authors would like to thank Victor Lobanov,Mingsheng Jia,and Hira Khan for critically reading the manuscript.
文摘Biogenic palladium nanoparticles(bio-Pd NPs)are used for the reductive transformation and/or dehalogenation of persistent micropollutants.In this work,H2(electron donor)was produced in situ by an electrochemical cell,permitting steered production of differently sized bio-Pd NPs.The catalytic activity was first assessed by the degradation of methyl orange.The NPs showing the highest catalytic activity were selected for the removal of micropollutants from secondary treated municipal wastewater.The synthesis at different H2 flow rates(0.310 L/hr or 0.646 L/hr)influenced the bio-Pd NPs size.The NPs produced over 6 hr at a lowH2 flow rate had a larger size(D50=39.0 nm)than those produced in 3 hr at a high H2 flow rate(D50=23.2 nm).Removal of 92.1%and 44.3%of methyl orange was obtained after 30 min for the NPs with sizes of 39.0 nm and 23.2 nm,respectively.Bio-Pd NPs of 39.0 nm were used to treat micropollutants present in secondary treated municipal wastewater at concentrations ranging fromμg/L to ng/L.Effective removal of 8 compounds was observed:ibuprofen(69.5%)<sulfamethoxazole(80.6%)<naproxen(81.4%)<furosemide(89.7%)<citalopram(91.7%)<diclofenac(91.9%)<atorvastatin(>94.3%)<lorazepam(97.2%).Re-moval of fluorinated antibiotics occurred at>90%efficiency.Overall,these data indicate that the size,and thus the catalytic activity of the NPs can be steered and that the removal of challengingmicropollutants at environmentally relevant concentrations can be achieved through the use of bio-Pd NPs.
基金supported by the National Natural Science Foundation of China(No.42107242 and 51974039)Chongqing Special Support Fund for Post Doctorsupported by a Competitive Research Grant from the Office of Sponsored Research(No.OSR-2016-CRG5-2985)of King Abdullah University of Science and Technology.
文摘Microbial electrosynthesis(MES)enables the bioproduction of multicarbon compounds from CO_(2)using electricity as the driver.Although high salinity can improve the energetic performance of bioelectrochemical systems,acetogenic processes under elevated salinity are poorly known.Here MES under 35e60 g L^(-1)salinity was evaluated.Acetate production in two-chamber MES systems at 35 g L^(-1)salinity(seawater composition)gradually decreased within 60 days,both under-1.2 V cathode potential(vs.Ag/AgCl)and^(-1).56 A m^(-2)reductive current.Carbonate precipitation on cathodes(mostly CaCO3)likely declined the production through inhibiting CO_(2)supply,the direct electrode contact for acetogens and H2 production.Upon decreasing Ca2t and Mg2t levels in three-chamber reactors,acetate was stably produced over 137 days along with a low cathode apparent resistance at 1.9±0.6 mU m^(2)and an average production rate at 3.80±0.21 g m^(-2)d^(-1).Increasing the salinity step-wise from 35 to 60 g L^(-1)gave the most efficient acetate production at 40 g L^(-1)salinity with average rates of acetate production and CO_(2)consumption at 4.56±3.09 and 7.02±4.75 g m^(-2)d^(-1),respectively.The instantaneous coulombic efficiency for VFA averaged 55.1±31.4%.Acetate production dropped at higher salinity likely due to the inhibited CO_(2)dissolution and acetogenic metabolism.Acetobacterium up to 78%was enriched on cathodes as the main acetogen at 35 g L^(-1).Under high-salinity selection,96.5%Acetobacterium dominated on the cathode along with 34.0%Sphaerochaeta in catholyte.This research provides a first proof of concept that MES starting from CO_(2)reduction can be achieved at elevated salinity.
基金Tim Lacoere and Sam Eggeling are kindly acknowledged for help with the artwork.Marlee Wasserman is kindly acknowledged for the English editing of the manuscript.This work was supported by the Fund Ernest Solvay,managed by the King Baudouin Foundation,under grant 2020-B7120700-214824SM is supported by the Bijzonder Onderzoeksfonds(BOF18/DOS/035)scholarship and ESPOLAP is supported by a Ghent University Bijzonder Onderzoeksfonds GOA grant(BOF19/GOA/026).
文摘Seasonal or permanent water scarcity in off-grid communities can be alleviated by recycling water in decentralized wastewater treatment systems.Nature-based solutions,such as constructed wetlands(CWs),have become popular solutions for sanitation in remote locations.Although typical CWs can efficiently remove solids and organics to meet water reuse standards,polishing remains necessary for other parameters,such as pathogens,nutrients,and recalcitrant pollutants.Different CW designs and CWs coupled with electrochemical technologies have been proposed to improve treatment efficiency.Electrochemical systems(ECs)have been either implemented within the CW bed(ECin-CW)or as a stage in a sequential treatment(CW+EC).A large body of literature has focused on ECin-CW,and multiple scaled-up systems have recently been successfully implemented,primarily to remove recalcitrant organics.Conversely,only a few reports have explored the opportunity to polish CW effluents in a downstream electrochemical module for the electro-oxidation of micropollutants or electro-disinfection of pathogens to meet more stringent water reuse standards.This paper aims to critically review the opportunities,challenges,and future research directions of the different couplings of CW with EC as a decentralized technology for water treatment and recovery.
基金supported as postdoctoral fellow by the Research Foundation Flanders(FWO-Vlaanderen).
文摘The anaerobic digestion process has been one of the key processes for renewable energy recovery from organic waste streams for over a century.The anaerobic digestion microbiome is,through the continuous development of novel techniques,evolving from a black box to a well-defined consortium,but we are not there yet.In this perspective,I provide my view on the current status and challenges of the anaerobic digestion microbiome,as well as the opportunities and solutions to exploit it.I consider identification and fingerprinting of the anaerobic digestion microbiome as complementary tools to monitor the anaerobic digestion microbiome.However,data availability,method-inherent biases and correct taxa identification hamper the accuracy and reproducibility of anaerobic digestion microbiome characterization.Standardisation of microbiome research in anaerobic digestion and other engineered systems will be essential in the coming decades,for which I proposed some targeted solutions.These will bring anaerobic digestion from a single-purpose energy-recovery technology to a versatile process for integrated resource recovery.It is my opinion that the exploitation of the microbiome will be a driver of innovation,and that it has a key role to play in the bio-based economy of the decades to come.
基金supported by the People Programme(Marie Curie Actions)of the 7th Framework Programme of the European Union(FP7/2007-2013)under the REA grant agreement no.600388(TECNIOspring programme)the Agency for Business Competitiveness of the Government of Catalonia(ACCIO)+1 种基金financial support from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 767678supported by the Fonds voor Wetenschappelijk Onderzoek FWO via project MiCrO2(Grant number G020616N).
文摘are time-consuming and not sensitive enough.However,bacteria typically connect to electrodes through biofilm formation,leading to problems due to lack of uniformity or long device production times.A suitable immobilisation technique can overcome these challenges.Still,they may respond more slowly than biofilm-based electrodes because bacteria gradually adapt to electron transfer during biofilm formation.In this study,we propose a controlled and reproducible way to fabricate bacteria-modified electrodes.The method consists of an immobilisation step using a cellulose matrix,followed by an electrode polarization in the presence of ferricyanide and glucose.Our process is short,reproducible and led us to obtain ready-to-use electrodes featuring a high-current response.An excellent shelf-life of the immobilised electrochemically active bacteria was demonstrated for up to one year.After an initial 50% activity loss in the first month,no further declines have been observed over the following 11 months.We implemented our bacteria-modified electrodes to fabricate a lateral flow platform for toxicity monitoring using formaldehyde(3%).Its addition led to a 59% current decrease approximately 20 min after the toxic input.The methods presented here offer the ability to develop a high sensitivity,easy to produce,and long shelf life bacteria-based toxicity detectors.