This study describes the application of electrospray ionization mass spectrometry （ESI-MS） to investigate copper ion interaction with amoxicillin. ESI mass spectra of Cu-amoxicillin complexes show complex ions at m/...This study describes the application of electrospray ionization mass spectrometry （ESI-MS） to investigate copper ion interaction with amoxicillin. ESI mass spectra of Cu-amoxicillin complexes show complex ions at m/z 828, 792, 753, 731,428, 388 and 366 corresponding to [63Cu＋（2A-H）＋2H2O]＋, [63Cu＋（2A-H）]＋, [2A＋Na]＋, [2A＋H]＊, [63Cu＋（A-H）]~, [A＋Na]~ and [A＋H]＋ （where A = amoxicillin）. Based on the observed m/z values of Cu-amoxicillin complex ions, it is found that the Cu-amoxicillin ratios are 1：1 and 1：2, and the copper ions exhibited three feasible coordination numbers （2, 4 and 6） with arnoxicillin complexes. The structures and coordination numbers of copper-arnoxicillin complex ions were probed from their collisionally activated dissociation （CAD） spectra. Based on these results, it is confirmed that the copper ions could form stable tetrahedral and octahedral complexes with amoxicillin. This study validates the annlicabilitv of ESI-MS for nrobin; CODDer-amoxicillin comDlex ions.展开更多
基金National Science Council(NSC)forfinancial support
文摘This study describes the application of electrospray ionization mass spectrometry （ESI-MS） to investigate copper ion interaction with amoxicillin. ESI mass spectra of Cu-amoxicillin complexes show complex ions at m/z 828, 792, 753, 731,428, 388 and 366 corresponding to [63Cu＋（2A-H）＋2H2O]＋, [63Cu＋（2A-H）]＋, [2A＋Na]＋, [2A＋H]＊, [63Cu＋（A-H）]~, [A＋Na]~ and [A＋H]＋ （where A = amoxicillin）. Based on the observed m/z values of Cu-amoxicillin complex ions, it is found that the Cu-amoxicillin ratios are 1：1 and 1：2, and the copper ions exhibited three feasible coordination numbers （2, 4 and 6） with arnoxicillin complexes. The structures and coordination numbers of copper-arnoxicillin complex ions were probed from their collisionally activated dissociation （CAD） spectra. Based on these results, it is confirmed that the copper ions could form stable tetrahedral and octahedral complexes with amoxicillin. This study validates the annlicabilitv of ESI-MS for nrobin; CODDer-amoxicillin comDlex ions.
