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In vivo imaging of the neuronal response to spinal cord injury:a narrative review
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作者 Junhao Deng Chang Sun +5 位作者 Ying Zheng Jianpeng Gao Xiang Cui Yu Wang Licheng Zhang Peifu Tang 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第4期811-817,共7页
Deciphering the neuronal response to injury in the spinal cord is essential for exploring treatment strategies for spinal cord injury(SCI).However,this subject has been neglected in part because appropriate tools are ... Deciphering the neuronal response to injury in the spinal cord is essential for exploring treatment strategies for spinal cord injury(SCI).However,this subject has been neglected in part because appropriate tools are lacking.Emerging in vivo imaging and labeling methods offer great potential for observing dynamic neural processes in the central nervous system in conditions of health and disease.This review first discusses in vivo imaging of the mouse spinal cord with a focus on the latest imaging techniques,and then analyzes the dynamic biological response of spinal cord sensory and motor neurons to SCI.We then summarize and compare the techniques behind these studies and clarify the advantages of in vivo imaging compared with traditional neuroscience examinations.Finally,we identify the challenges and possible solutions for spinal cord neuron imaging. 展开更多
关键词 anterior horn neurons calcium imaging central nervous system dorsal horn neurons dorsal root ganglion in vivo imaging neuronal response spinal cord injury spinal cord two-photon microscopy
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Epidemiological and clinical features,treatment status,and economic burden of traumatic spinal cord injury in China:a hospital-based retrospective study 被引量:4
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作者 Hengxing Zhou Yongfu Lou +32 位作者 Lingxiao Chen Yi Kang Lu Liu Zhiwei Cai David BAnderson Wei Wang Chi Zhang Jinghua Wang Guangzhi Ning Yanzheng Gao Baorong He Wenyuan Ding Yisheng Wang Wei Mei Yueming Song Yue Zhou Maosheng Xia Huan Wang Jie Zhao Guoyong Yin Tao Zhang Feng Jing Rusen Zhu Bin Meng Li Duan Zhongmin Zhang Desheng Wu Zhengdong Cai Lin Huang Zhanhai Yin Kainan Li Shibao Lu Shiqing Feng 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第5期1126-1132,共7页
Traumatic spinal cord injury is potentially catastrophic and can lead to permanent disability or even death.China has the largest population of patients with traumatic spinal cord injury.Previous studies of traumatic ... Traumatic spinal cord injury is potentially catastrophic and can lead to permanent disability or even death.China has the largest population of patients with traumatic spinal cord injury.Previous studies of traumatic spinal cord injury in China have mostly been regional in scope;national-level studies have been rare.To the best of our knowledge,no national-level study of treatment status and economic burden has been performed.This retrospective study aimed to examine the epidemiological and clinical features,treatment status,and economic burden of traumatic spinal cord injury in China at the national level.We included 13,465 traumatic spinal cord injury patients who were injured between January 2013 and December 2018 and treated in 30 hospitals in 11 provinces/municipalities representing all geographical divisions of China.Patient epidemiological and clinical features,treatment status,and total and daily costs were recorded.Trends in the percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department and cost of care were assessed by annual percentage change using the Joinpoint Regression Program.The percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department did not significantly change overall(annual percentage change,-0.5%and 2.1%,respectively).A total of 10,053(74.7%)patients underwent surgery.Only 2.8%of patients who underwent surgery did so within 24 hours of injury.A total of 2005(14.9%)patients were treated with high-dose(≥500 mg)methylprednisolone sodium succinate/methylprednisolone(MPSS/MP);615(4.6%)received it within 8 hours.The total cost for acute traumatic spinal cord injury decreased over the study period(-4.7%),while daily cost did not significantly change(1.0%increase).Our findings indicate that public health initiatives should aim at improving hospitals’ability to complete early surgery within 24 hours,which is associated with improved sensorimotor recovery,increasing the awareness rate of clinical guidelines related to high-dose MPSS/MP to reduce the use of the treatment with insufficient evidence. 展开更多
关键词 China clinical features COSTS EPIDEMIOLOGY methylprednisolone sodium succinate METHYLPREDNISOLONE retrospective study traumatic spinal cord injury TREATMENT
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Application value of biofluid-based biomarkers for the diagnosis and treatment of spinal cord injury 被引量:4
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作者 Hong-Da Wang Zhi-Jian Wei +1 位作者 Jun-Jin Li Shi-Qing Feng 《Neural Regeneration Research》 SCIE CAS CSCD 2022年第5期963-971,共9页
Recent studies in patients with spinal cord injuries(SCIs)have confirmed the diagnostic potential of biofluid-based biomarkers,as a topic of increasing interest in relation to SCI diagnosis and treatment.This paper re... Recent studies in patients with spinal cord injuries(SCIs)have confirmed the diagnostic potential of biofluid-based biomarkers,as a topic of increasing interest in relation to SCI diagnosis and treatment.This paper reviews the research progress and application prospects of recently identified SCI-related biomarkers.Many structural proteins,such as glial fibrillary acidic protein,S100-β,ubiquitin carboxy-terminal hydrolase-L1,neurofilament light,and tau protein were correlated with the diagnosis,American Spinal Injury Association Impairment Scale,and prognosis of SCI to different degrees.Inflammatory factors,including interleukin-6,interleukin-8,and tumor necrosis factorα,are also good biomarkers for the diagnosis of acute and chronic SCI,while non-coding RNAs(micro RNAs and long non-coding RNAs)also show diagnostic potential for SCI.Trace elements(Mg,Se,Cu,Zn)have been shown to be related to motor recovery and can predict motor function after SCI,while humoral markers can reflect the pathophysiological changes after SCI.These factors have the advantages of low cost,convenient sampling,and ease of dynamic tracking,but are also associated with disadvantages,including diverse influencing factors and complex level changes.Although various proteins have been verified as potential biomarkers for SCI,more convincing evidence from large clinical and prospective studies is thus required to identify the most valuable diagnostic and prognostic biomarkers for SCI. 展开更多
关键词 BIOMARKER diagnosis inflammatory cytokine motor recovery non-coding RNA PROGNOSIS spinal cord injury structural protein trace element
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Ferroptosis inhibition protects vascular endothelial cells and maintains integrity of the blood-spinal cord barrier after spinal cord injury 被引量:3
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作者 Wenxiang Li Xiaoqing Zhao +12 位作者 Rong Zhang Xinjie Liu Zhangyang Qi Yang Zhang Weiqi Yang Yilin Pang Chenxi Zhao Baoyou Fan Ning Ran Jiawei Zhang Xiaohong Kong Shiqing Feng Xue Yao 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第11期2474-2481,共8页
Maintaining the integrity of the blood-spinal cord barrier is critical for the recove ry of spinal cord injury.Ferro ptosis contributes to the pathogenesis of spinal cord injury.We hypothesized that ferroptosis is inv... Maintaining the integrity of the blood-spinal cord barrier is critical for the recove ry of spinal cord injury.Ferro ptosis contributes to the pathogenesis of spinal cord injury.We hypothesized that ferroptosis is involved in disruption of the blood-s pinal cord barrier.In this study,we administe red the ferroptosis inhibitor liproxstatin-1 intraperitoneally after contusive spinal co rd injury in rats.Liproxstatin-1 improved locomotor recovery and somatosensory evoked potential electrophysiological performance after spinal cord inju ry.Liproxstatin-1 maintained blood-spinal cord barrier integrity by upregulation of the expression of tight junction protein.Liproxstatin-1 inhibited ferroptosis of endothelial cell after spinal cord injury,as shown by the immunofluorescence of an endothelial cell marker(rat endothelium cell antigen-1,RECA-1) and fe rroptosis markers Acyl-CoA synthetase long-chain family member 4 and 15-lipoxygenase.Liproxstatin-1reduced brain endothelial cell ferroptosis in vitro by upregulating glutathione peroxidase 4 and downregulating Acyl-CoA synthetase long-chain family member4 and 15-lipoxygenase.Furthermore,inflammatory cell recruitment and astrogliosis were mitigated after liproxstatin-1 treatment.In summary,liproxstatin-1im proved spinal cord injury recovery by inhibiting ferroptosis in endothelial cells and maintaining blood-s pinal co rd barrier integrity. 展开更多
关键词 blood-spinal cord barrier ferroptosis liproxstatin-1 NEUROINFLAMMATION spinal cord injury vascular endothelial cells
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Adhesive hydrogels in osteoarthritis:from design to application 被引量:2
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作者 Wang-Lin Duan Li-Ning Zhang +6 位作者 Raghvendra Bohara Sergio Martin-Saldana Fei Yang Yi-Yang Zhao Yong Xie Ya-Zhong Bu Abhay Pandit 《Military Medical Research》 SCIE CAS CSCD 2023年第5期652-679,共28页
Osteoarthritis(OA)is the most common type of degenerative joint disease which affects 7%of the global population and more than 500 million people worldwide.One research frontier is the development of hydrogels for OA ... Osteoarthritis(OA)is the most common type of degenerative joint disease which affects 7%of the global population and more than 500 million people worldwide.One research frontier is the development of hydrogels for OA treatment,which operate either as functional scaffolds of tissue engineering or as delivery vehicles of functional additives.Both approaches address the big challenge:establishing stable integration of such delivery systems or implants.Adhesive hydrogels provide possible solutions to this challenge.However,few studies have described the current advances in using adhesive hydrogel for OA treatment.This review summarizes the commonly used hydrogels with their adhesion mechanisms and components.Additionally,recognizing that OA is a complex disease involving different biological mechanisms,the bioactive therapeutic strategies are also presented.By presenting the adhesive hydrogels in an interdisciplinary way,including both the fields of chemistry and biology,this review will attempt to provide a comprehensive insight for designing novel bioadhesive systems for OA therapy. 展开更多
关键词 Adhesive hydrogel OSTEOARTHRITIS Functional additives Cartilage regeneration Interdisciplinary therapy
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Clinical guidelines for indications,techniques,and complications of autogenous bone grafting
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作者 Jianzheng Zhang Shaoguang Li +17 位作者 Hongying He Li Han Simeng Zhang Lin Yang Wenxing Han Xiaowei Wang Jie Gao Jianwen Zhao Weidong Shi Zhuo Wu Hao Wang Zhicheng Zhang Licheng Zhang Wei Chen Qingtang Zhu Tiansheng Sun Peifu Tang Yingze Zhang 《Chinese Medical Journal》 SCIE CAS CSCD 2024年第1期5-7,共3页
Autogenous bone grafts have long been considered the“gold standard”and most effective material in bone regeneration procedures.[1]Autogenous bone grafts are used to repair bone defects caused by nonunion,infection,t... Autogenous bone grafts have long been considered the“gold standard”and most effective material in bone regeneration procedures.[1]Autogenous bone grafts are used to repair bone defects caused by nonunion,infection,tumor resection,and spinal and joint fusion.[2]It has been reported that more than 200,000 autologous bone grafts are performed in the United States each year.[3]Although there are no specific statistics on the annual number of bone grafts performed in China,autologous bone grafting is the most common surgical technique in orthopedics.The iliac crest remains the most common donor site,along with the fibula,ribs,tibial metaphysis,proximal humerus,distal radius,and greater trochanter.[4,5]Various bone-graft options provide different amounts and qualities of cortical,cancellous,and corticocancellous bone.[6,7]Autogenous bone graft is osteogenic,histocompatible,provides structural support. 展开更多
关键词 GRAFTING GRAFT UNION
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Self-rectifying magnetoelectric device for remote neural regeneration and function restoration
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作者 Yuanhao Tong Yuanming Ouyang +1 位作者 Cunyi Fan Yun Qian 《Biomaterials Translational》 2024年第2期197-199,共3页
Recently,Joshua C.Chen,Gauri Bhave,and Jacob T.Robinson from Rice University reported a magnetoelectric nonlinear metamaterial(MNM)for neural signal transmission and nerve function restoration.1 Nonlinear charge trans... Recently,Joshua C.Chen,Gauri Bhave,and Jacob T.Robinson from Rice University reported a magnetoelectric nonlinear metamaterial(MNM)for neural signal transmission and nerve function restoration.1 Nonlinear charge transport between the semiconductor layers enabled this magnetoelectric(ME)metamaterial to have a nonlinear ME coupling coefficient,which allowed for self-rectification(Figure 1). 展开更多
关键词 FUNCTION MAGNETO NONLINEAR
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Skeleton-vasculature chain reaction: a novel insight into the mystery of homeostasis 被引量:6
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作者 Ming Chen Yi Li +5 位作者 Xiang Huang Ya Gu Shang Li Pengbin Yin Licheng Zhang Peifu Tang 《Bone Research》 SCIE CAS CSCD 2021年第3期264-283,共20页
Angiogenesis and osteogenesis are coupled.However,the cellular and molecular regulation of these processes remains to be further investigated.Both tissues have recently been recognized as endocrine organs,which has st... Angiogenesis and osteogenesis are coupled.However,the cellular and molecular regulation of these processes remains to be further investigated.Both tissues have recently been recognized as endocrine organs,which has stimulated research interest in the screening and functional identification of novel paracrine factors from both tissues.This review aims to elaborate on the novelty and significance of endocrine regulatory loops between bone and the vasculature.In addition,research progress related to the bone vasculature,vessel-related skeletal diseases,pathological conditions,and angiogenesis-targeted therapeutic strategies are also summarized.With respect to future perspectives,new techniques such as single-cell sequencing,which can be used to show the cellular diversity and plasticity of both tissues,are facilitating progress in this field.Moreover,extracellular vesicle-mediated nuclear acid communication deserves further investigation.In conclusion,a deeper understanding of the cellular and molecular regulation of angiogenesis and osteogenesis coupling may offer an opportunity to identify new therapeutic targets. 展开更多
关键词 HOMEOSTASIS ORGANS diseases
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Chitin scaffold combined with autologous small nerve repairs sciatic nerve defects 被引量:3
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作者 Bo Wang Chang-Feng Lu +5 位作者 Zhong-Yang Liu Shuai Han Pi Wei Dian-Ying Zhang Yu-Hui Kou Bao-Guo Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2022年第5期1106-1114,共9页
Although autologous nerve transplantation is the gold standard for treating peripheral nerve defects,it has many clinical limitations.As an alternative,various tissue-engineered nerve grafts have been developed to sub... Although autologous nerve transplantation is the gold standard for treating peripheral nerve defects,it has many clinical limitations.As an alternative,various tissue-engineered nerve grafts have been developed to substitute for autologous nerves.In this study,a novel nerve graft composed of chitin scaffolds and a small autologous nerve was used to repair sciatic nerve defects in rats.The novel nerve graft greatly facilitated regeneration of the sciatic nerve and myelin sheath,reduced atrophy of the target muscle,and effectively restored neurological function.When the epineurium of the small autogenous nerve was removed,the degree of nerve regeneration was similar to that which occurs after autogenous nerve transplantation.These findings suggest that our novel nerve graft might eventually be a new option for the construction of tissue-engineered nerve scaffolds.The study was approved by the Research Ethics Committee of Peking University People's Hospital(approval No.2019 PHE27)on October 18,2019. 展开更多
关键词 autologous small nerve chitin scaffold nerve defect nervous system peripheral nerve injury peripheral nerve regeneration sciatic nerve TRAUMA
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Early patellar tendon rupture after total knee arthroplasty: A direct repair method 被引量:3
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作者 Tie-Jian Li Jing-Yang Sun +3 位作者 Yin-Qiao Du Jun-Min Shen Bo-Han Zhang Yong-Gang Zhou 《World Journal of Clinical Cases》 SCIE 2022年第31期11349-11357,共9页
BACKGROUND Patellar tendon rupture after total knee arthroplasty(TKA)is a catastrophic complication.Although the occurrence of this injury is rare,it can lead to significant dysfunction for the patient and is very tri... BACKGROUND Patellar tendon rupture after total knee arthroplasty(TKA)is a catastrophic complication.Although the occurrence of this injury is rare,it can lead to significant dysfunction for the patient and is very tricky to deal with.There has been no standard treatment for early patella tendon rupture after TKA,and long-term follow-up data are lacking.AIM To introduce a direct repair method for early patella tendon rupture following TKA and determine the clinical outcomes and complications of this method.METHODS During the period of 2008 to 2021,3265 consecutive TKAs were retrospectively reviewed.Twelve patients developed early patellar tendon rupture postoperatively and were treated by a direct repair method.Mean follow-up was 5.7 years.Demographic,operative,and clinical data were collected.The clinical outcomes were assessed using the Western Ontario and McMaster Universities(WOMAC)score,the Hospital for Special Surgery(HSS)score,knee range of motion,extensor lag,and surgical complications.Descriptive statistics and paired t test were employed to analyze the data.RESULTS For all 12 patients who underwent direct repair for early patellar tendon rupture,3 patients failed:One(8.3%)for infection and two(17.6%)for re-fracture.The two patients with re-fracture both underwent reoperation to reconstruct the extensor mechanism and the patient with infection underwent revision surgery.The range of motion was 109.2°±10.6°preoperatively to 87.9°±11°postoperatively,mean extensor lag was 21°at follow-up,and mean WOMAC and HSS scores were 65.8±30.9 and 60.3±21.7 points,respectively.CONCLUSION This direct repair method of early patellar tendon rupture is not an ideal therapy.It is actually ineffective for the recovery of knee joint function in patients,and is still associated with severe knee extension lag and high complication rates.Compared with the outcomes of other repair methods mentioned in the literature,this direct repair method shows poor clinical outcomes. 展开更多
关键词 Direct repair Patellar tendon fracture Total knee arthroplasty RECONSTRUCTION High complication rates
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Bone microenvironment regulative hydrogels with ROS scavenging and prolonged oxygen-generating for enhancing bone repair 被引量:9
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作者 Han Sun Juan Xu +4 位作者 Yangyufan Wang Siyu Shen Xingquan Xu Lei Zhang Qing Jiang 《Bioactive Materials》 SCIE CSCD 2023年第6期477-496,共20页
Large bone defects resulting from fractures and disease are a major clinical challenge,being often unable to heal spontaneously by the body’s repair mechanisms.Lines of evidence have shown that hypoxia-induced overpr... Large bone defects resulting from fractures and disease are a major clinical challenge,being often unable to heal spontaneously by the body’s repair mechanisms.Lines of evidence have shown that hypoxia-induced overproduction of ROS in bone defect region has a major impact on delaying bone regeneration.However,replenishing excess oxygen in a short time cause high oxygen tension that affect the activity of osteoblast precursor cells.Therefore,reasonably restoring the hypoxic condition of bone microenvironment is essential for facilitating bone repair.Herein,we designed ROS scavenging and responsive prolonged oxygen-generating hydrogels(CPP-L/GelMA)as a“bone microenvironment regulative hydrogel”to reverse the hypoxic microenvironment in bone defects region.CPP-L/GelMA hydrogels comprises an antioxidant enzyme catalase(CAT)and ROS-responsive oxygen-releasing nanoparticles(PFC@PLGA/PPS)co-loaded liposome(CCP-L)and GelMA hydrogels.Under hypoxic condition,CPP-L/GelMA can release CAT for degrading hydrogen peroxide to generate oxygen and be triggered by superfluous ROS to continuously release the oxygen for more than 2 weeks.The prolonged oxygen enriched microenvironment generated by CPP-L/GelMA hydrogel significantly enhanced angiogenesis and osteogenesis while inhibited osteoclastogenesis.Finally,CPP-L/GelMA showed excellent bone regeneration effect in a mice skull defect model through the Nrf2-BMAL1-autophagy pathway.Hence,CPP-L/GelMA,as a bone microenvironment regulative hydrogel for bone tissue respiration,can effectively scavenge ROS and provide prolonged oxygen supply according to the demand in bone defect region,possessing of great clinical therapeutic potential. 展开更多
关键词 Bone defect Hypoxic microenvironment Reactive oxygen species responsiveness Prolonged oxygen generation Brain and muscle arnt-like protein 1
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3D-printed fish gelatin scaffolds for cartilage tissue engineering 被引量:2
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作者 Abudureheman Maihemuti Han Zhang +4 位作者 Xiang Lin Yangyufan Wang Zhihong Xu Dagan Zhang Qing Jiang 《Bioactive Materials》 SCIE CSCD 2023年第8期77-87,共11页
Knee osteoarthritis is a chronic disease caused by the deterioration of the knee joint due to various factors such as aging,trauma,and obesity,and the nonrenewable nature of the injured cartilage makes the treatment o... Knee osteoarthritis is a chronic disease caused by the deterioration of the knee joint due to various factors such as aging,trauma,and obesity,and the nonrenewable nature of the injured cartilage makes the treatment of osteoarthritis challenging.Here,we present a three-dimensional(3D)printed porous multilayer scaffold based on cold-water fish skin gelatin for osteoarticular cartilage regeneration.To make the scaffold,cold-water fish skin gelatin was combined with sodium alginate to increase viscosity,printability,and mechanical strength,and the hybrid hydrogel was printed according to a pre-designed specific structure using 3D printing technology.Then,the printed scaffolds underwent a double-crosslinking process to enhance their mechanical strength even further.These scaffolds mimic the structure of the original cartilage network in a way that allows chondrocytes to adhere,proliferate,and communicate with each other,transport nutrients,and prevent further damage to the joint.More importantly,we found that cold-water fish gelatin scaffolds were nonimmunogenic,nontoxic,and biodegradable.We also implanted the scaffold into defective rat cartilage for 12 weeks and achieved satisfactory repair results in this animal model.Thus,cold-water fish skin gelatin scaffolds may have broad application potential in regenerative medicine. 展开更多
关键词 3D printing Fish skin gelatin Sodium alginate Cartilage defect repair Tissue engineering
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Fish scale-derived scaffolds with MSCs loading for photothermal therapy of bone defect 被引量:1
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作者 Siyu Shen Rui Liu +5 位作者 Chuanhui Song Tao Shen Yiwen Zhou Junxia Guo Bin Kong Qing Jiang 《Nano Research》 SCIE EI CSCD 2023年第5期7383-7392,共10页
Tissue engineering scaffolds have presented effective value in bone repair.However,the integration of the diverse components,complex structures,multifunction to impart the scaffolds with improved applicability is stil... Tissue engineering scaffolds have presented effective value in bone repair.However,the integration of the diverse components,complex structures,multifunction to impart the scaffolds with improved applicability is still a challenge.Here,we propose a novel fish-derived scaffold combined with photothermal therapy and mesenchymal stem cells(MSCs)to promote bone regeneration.The fish-derived scaffold is composed of the decellularized fish scale and gelatin methacrylate synthesized from fish gelatin(fGelMA),which can promote the proliferation and osteogenesis of MSCs with no obvious immunological rejection.Furthermore,the black phosphorus(BP)nanosheets are incorporated into the fGelMA hydrogel network,which can endow the hydrogel with the capacity of photothermal conversion stimulated by near-infrared(NIR)light.The fish-derived scaffold can promote the osteogenesis process of MSCs with higher expression of osteogenic markers and higher mineralization assisted by the NIR light in vitro.The regeneration of mice calvarial defect has also been accelerated by the scaffold with photothermal therapy and MSCs.These results suggest that the fish-derived scaffold,photothermal therapy,MSCs-based regenerative therapy is a promising clinical strategy in bone regeneration. 展开更多
关键词 fish scale GelMA photothermal therapy mesenchymal stem cell bone regeneration
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Chinese expert consensus on organ protection of transplantation(2022 edition)
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作者 Jian-Hui Li Xiao Xu +30 位作者 Yan-Feng Wang Hai-Yang Xie Jing-Yu Chen Nian-Guo Dong Mitesh Badiwala Li-Ming Xin Roberto Vanin Pinto Ribeiro Hao Yin Hao Zhang Jian-Zheng Zhang Feng Huo Jia-Yin Yang Hong-Ji Yang Hui Pan Shao-Guang Li Yin-Biao Qiao Jia Luo Hao-Yu Li Jun-Jun Jia Hao Yu Han Liang Si-Jia Yang Hao Wang Zhong-Yang Liu Li-Cheng Zhang Xiao-Yi Hu Hao Wu Yi-Qing Hu Pei-Fu Tang Qi-Fa Ye Shu-Sen Zheng 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS CSCD 2022年第6期516-526,共11页
Introduction Organ transplantation increases survival and improves qual-ity of life to many patients with end-stage organ failure.Or-gan shortage is a worldwide problem that restricts organ trans-plantation[1].Organ p... Introduction Organ transplantation increases survival and improves qual-ity of life to many patients with end-stage organ failure.Or-gan shortage is a worldwide problem that restricts organ trans-plantation[1].Organ procurement and preservation as well as ischemia-reperfusion injury(IRI)after transplantation are the im-portant factors affecting prognosis of recipients.Since the de-velopment of organ transplantation technology in the 20th cen-tury,organ protection technology has been a most promising con-cept in this field.Organ preservation solutions such as the Collins solution,University of Wisconsin(UW)solution,and histidine-tryptophan-ketoglutarate(HTK)solution were developed sequen-tially[2],which developed rapidly in static cold storage(SCS)tech-niques.SCS remains the standard preservation technique for organ transplantation[2]. 展开更多
关键词 ORGAN PRESERVATION TRANSPLANTATION
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Liposomalα-cyperone targeting bone resorption surfaces suppresses osteoclast differentiation and osteoporosis progression via the PI3K/Akt axis
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作者 Lin Yang Xueying An +7 位作者 Wang Gong Wenshu Wu Bin Liu Xiaoyan Shao Yansi Xian Rui Peng Baosheng Guo Qing Jiang 《Nano Research》 SCIE EI CSCD 2024年第4期2949-2959,共11页
Osteoporosis is a metabolic dysregulation of bone that occurs mainly in postmenopausal women,and the hyperfunction of osteoclasts is the primary contributor to postmenopausal osteoporosis.However,the development of ef... Osteoporosis is a metabolic dysregulation of bone that occurs mainly in postmenopausal women,and the hyperfunction of osteoclasts is the primary contributor to postmenopausal osteoporosis.However,the development of effective therapeutic drugs and precise delivery systems remains a challenge in the field of anti-absorption therapy.Here,we reported theα-cyperone(α-CYP)for anti-osteoporosis and developed a liposome-based nano-drug delivery system ofα-CYP,that specifically targets the bone resorption interface.Firstly,we found that theα-CYP,one of the major sesquiterpenes of Cyperus rotundus L.,attenuated the progression of osteoporosis in ovariectomized(OVX)mice and down-regulated the expression of phosphorylated proteins of phosphoinositide 3-kinase(PI3K)and protein kinase B(Akt),causing down-regulation of osteoclast-related genes/proteins and curbing osteoclast differentiation.Furthermore,α-CYP reversed the activation of osteoclastic differentiation and enhanced osteoporosis-related proteins expression caused by PI3K/Akt agonist(YS-49).More importantly,we adopted the osteoclastic resorption surface targeting peptide Asp8 and constructed the liposome(lipαC@Asp8)to deliverα-CYP to osteoclasts and confirmed its anti-osteoporosis effect and enhanced osteoclast inhibition by blocking PI3K/Akt axis.In conclusion,this study demonstrated thatα-CYP inhibits osteoclast differentiation and osteoporosis development by silencing PI3K/Akt pathway,and the liposome targeting delivery systems loaded withα-CYP might provide a novel and effective strategy to treat osteoporosis. 展开更多
关键词 OSTEOPOROSIS Α-CYPERONE OSTEOCLAST phosphoinositide 3-kinase/protein kinase B(PI3K/Akt) liposome
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Bone targeting antioxidative nano-iron oxide for treating postmenopausal osteoporosis 被引量:4
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作者 Liming Zheng Zaikai Zhuang +7 位作者 Yixuan Li Tianshu Shi Kai Fu Wenjin Yan Lei Zhang Peng Wang Lan Li Qing Jiang 《Bioactive Materials》 SCIE 2022年第8期250-261,共12页
Osteoporosis is the most common degenerative orthopedic disease in the elderly.Recently,the therapeutic methods for osteoporosis have shifted towards the regulation of local immunity in bone tissues,which could provid... Osteoporosis is the most common degenerative orthopedic disease in the elderly.Recently,the therapeutic methods for osteoporosis have shifted towards the regulation of local immunity in bone tissues,which could provide a suitable environment for the positive regulation of bone metabolism,promoting osteogenic differentiation and inhibiting osteoclast differentiation.Our previous work demonstrated that iron oxide nanoparticles(IONPs)could positively regulate bone metabolism in vitro.In this study,we further demonstrated that daily administration of IONPs relieved estrogen deficiency-induced osteoporosis via scavenging reactive oxygen species in vivo.Meanwhile,IONPs promoted the osteogenic differentiation of bone marrow mesenchymal stem cells and inhibited the osteoclast differentiation of monocytes from IONPs treated mice.Besides,alendronate,a clinically used anti-osteoporosis bisphosphate,was employed to precisely deliver the IONPs to the bone tissues and played a synergically therapeutic role.Eventually,we verified the bone targeting ability,therapeutic efficiency,and biocompatibility of the novel bone target iron oxides in ovariectomy-induced osteoporotic mice.By applying BTNPs,the OVX-induced osteoporosis was significantly revised in mice models via the positive regulation of bone metabolism. 展开更多
关键词 OSTEOPOROSIS Bone targeting Iron oxide ANTIOXIDANT NANOMEDICINE
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Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering 被引量:4
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作者 Yong Tang Keyu Luo +13 位作者 Yin Chen Yueqi Chen Rui Zhou Can Chen Jiulin Tan Moyuan Deng Qijie Dai Xueke Yu Jian Liu Chengmin Zhang Wenjie Wu Jianzhong Xu Shiwu Dong Fei Luo 《Bioactive Materials》 SCIE 2021年第7期2039-2057,共19页
A close relationship has been reported to exist between cadherin-mediated cell-cell adhesion and integrin-mediated cell mobility,and protein tyrosine phosphatase 1B(PTP1B)may be involved in maintaining this homeostasi... A close relationship has been reported to exist between cadherin-mediated cell-cell adhesion and integrin-mediated cell mobility,and protein tyrosine phosphatase 1B(PTP1B)may be involved in maintaining this homeostasis.The stable residence of mesenchymal stem cells(MSCs)and endothelial cells(ECs)in their niches is closely related to the regulation of PTP1B.However,the exact role of the departure of MSCs and ECs from their niches during bone regeneration is largely unknown.Here,we show that the phosphorylation state of PTP1B tyrosine-152(Y152)plays a central role in initiating the departure of these cells from their niches and their subsequent recruitment to bone defects.Based on our previous design of a PTP1B Y152 region-mimicking peptide(152RM)that significantly inhibits the phosphorylation of PTP1B Y152,further investigations revealed that 152RM enhanced cell migration partly via integrinαvβ3 and promoted MSCs osteogenic differentiation partly by inhibiting ATF3.Moreover,152RM induced type H vessels formation by activating Notch signaling.Demineralized bone matrix(DBM)scaffolds were fabricated with mesoporous silica nanoparticles(MSNs),and 152RM was then loaded onto them by electrostatic adsorption.The DBM-MSN/152RM scaffolds were demonstrated to induce bone formation and type H vessels expansion in vivo.In conclusion,our data reveal that 152RM contributes to bone formation by coupling osteogenesis with angiogenesis,which may offer a potential therapeutic strategy for bone defects. 展开更多
关键词 PTP1B Bone regeneration ANGIOGENESIS Cell migration Type H vessels
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Cartilage lacuna-biomimetic hydrogel microspheres endowed with integrated biological signal boost endogenous articular cartilage regeneration
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作者 Hao Li Tianyuan Zhao +7 位作者 Zhiguo Yuan Tianze Gao Yongkang Yang Runmeng Li Qinyu Tian Peifu Tang Quanyi Guo Licheng Zhang 《Bioactive Materials》 SCIE 2024年第11期61-82,共22页
Despite numerous studies on chondrogenesis,the repair of cartilage—particularly the reconstruction of cartilage lacunae through an all-in-one advanced drug delivery system remains limited.In this study,we developed a... Despite numerous studies on chondrogenesis,the repair of cartilage—particularly the reconstruction of cartilage lacunae through an all-in-one advanced drug delivery system remains limited.In this study,we developed a cartilage lacuna-like hydrogel microsphere system endowed with integrated biological signals,enabling sequential immunomodulation and endogenous articular cartilage regeneration.We first integrated the chondrogenic growth factor transforming growth factor-β3(TGF-β3)into mesoporous silica nanoparticles(MSNs).Then,TGF-β3@MSNs and insulin-like growth factor 1(IGF-1)were encapsulated within microspheres made of polydopamine(pDA).In the final step,growth factor-loaded MSN@pDA and a chitosan(CS)hydrogel containing platelet-derived growth factor-BB(PDGF-BB)were blended to produce growth factors loaded composite microspheres(GFs@μS)using microfluidic technology.The presence of pDA reduced the initial acute inflammatory response,and the early,robust release of PDGF-BB aided in attracting endogenous stem cells.Over the subsequent weeks,the continuous release of IGF-1 and TGF-β3 amplified chondrogenesis and matrix formation.μS were incorporated into an acellular cartilage extracellular matrix(ACECM)and combined with a polydopamine-modified polycaprolactone(PCL)structure to produce a tissue-engineered scaffold that mimicked the structure of the cartilage lacunae evenly distributed in the cartilage matrix,resulting in enhanced cartilage repair and patellar cartilage protection.This research provides a strategic pathway for optimizing growth factor delivery and ensuring prolonged microenvironmental remodeling,leading to efficient articular cartilage regeneration. 展开更多
关键词 Microfluidic technology Hydrogel microsphere Immunomodulation Chondrogenesis Articular cartilage regeneration
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Promotion of osteochondral repair through immune microenvironment regulation and activation of endogenous chondrogenesis via the release of apoptotic vesicles from donor MSCs
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作者 Guangzhao Tian Han Yin +13 位作者 Jinxuan Zheng Rongcheng Yu Zhengang Ding Zineng Yan Yiqi Tang Jiang Wu Chao Ning Xun Yuan Chenxi Liao Xiang Sui Zhe Zhao Shuyun Liu Weimin Guo Quanyi Guo 《Bioactive Materials》 SCIE 2024年第11期455-470,共16页
Utilizing transplanted human umbilical cord mesenchymal stem cells(HUMSCs)for cartilage defects yielded advanced tissue regeneration,but the underlying mechanism remain elucidated.Early after HUMSCs delivery to the de... Utilizing transplanted human umbilical cord mesenchymal stem cells(HUMSCs)for cartilage defects yielded advanced tissue regeneration,but the underlying mechanism remain elucidated.Early after HUMSCs delivery to the defects,we observed substantial apoptosis.The released apoptotic vesicles(apoVs)of HUMSCs promoted cartilage regeneration by alleviating the chondro-immune microenvironment.ApoVs triggered M2 polarization in macrophages while simultaneously facilitating the chondrogenic differentiation of endogenous MSCs.Mechanistically,in macrophages,miR-100-5p delivered by apoVs activated the MAPK/ERK signaling pathway to promote M2 polarization.In MSCs,let-7i-5p delivered by apoVs promoted chondrogenic differentiation by targeting the eEF2K/p38 MAPK axis.Consequently,a cell-free cartilage regeneration strategy using apoVs combined with a decellularized cartilage extracellular matrix(DCM)scaffold effectively promoted the regeneration of osteochondral defects.Overall,new mechanisms of cartilage regeneration by transplanted MSCs were unconcealed in this study.Moreover,we provided a novel experimental basis for cell-free tissue engineering-based cartilage regeneration utilizing apoVs.Utilizing transplanted human umbilical cord mesenchymal stem cells(HUMSCs)for cartilage defects yielded advanced tissue regeneration,but the underlying mechanism remain elucidated.Early after HUMSCs delivery to the defects,we observed substantial apoptosis.The released apoptotic vesicles(apoVs)of HUMSCs promoted cartilage regeneration by alleviating the chondro-immune microenvironment.ApoVs triggered M2 polarization in macrophages while simultaneously facilitating the chondrogenic differentiation of endogenous MSCs.Mechanistically,in macrophages,miR-100-5p delivered by apoVs activated the MAPK/ERK signaling pathway to promote M2 polarization.In MSCs,let-7i-5p delivered by apoVs promoted chondrogenic differentiation by targeting the eEF2K/p38 MAPK axis.Consequently,a cell-free cartilage regeneration strategy using apoVs combined with a decellularized cartilage extracellular matrix(DCM)scaffold effectively promoted the regeneration of osteochondral defects.Overall,new mechanisms of cartilage regeneration by transplanted MSCs were unconcealed in this study.Moreover,we provided a novel experimental basis for cell-free tissue engineering-based cartilage regeneration utilizing apoVs. 展开更多
关键词 Apoptotic vesicles Cartilage regeneration Human umbilical cord mesenchymal stem cells Macrophage polarization Tissue engineering
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Histone deacetylase inhibition enhances extracellular vesicles from muscle to promote osteogenesis via miR-873-3p
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作者 Ming Chen Yi Li +21 位作者 Mingming Zhang Siliang Ge Taojin Feng Ruijing Chen Junmin Shen Ran Li Zhongqi Wang Yong Xie Duanyang Wang Jiang Liu Yuan Lin Feifan Chang Junyu Chen Xinyu Sun Dongliang Cheng Xiang Huang Fanfeng Wu Qinxiang Zhang Pingqiang Cai Pengbin Yin Licheng Zhang Peifu Tang 《Signal Transduction and Targeted Therapy》 SCIE 2024年第10期4730-4748,共19页
Regular physical activity is widely recognized for reducing the risk of various disorders,with skeletal muscles playing a key role by releasing biomolecules that benefit multiple organs and tissues.However,many indivi... Regular physical activity is widely recognized for reducing the risk of various disorders,with skeletal muscles playing a key role by releasing biomolecules that benefit multiple organs and tissues.However,many individuals,particularly the elderly and those with clinical conditions,are unable to engage in physical exercise,necessitating alternative strategies to stimulate muscle cells to secrete beneficial biomolecules.Histone acetylation and deacetylation significantly influence exercise-induced gene expression,suggesting that targeting histone deacetylases(HDACs)could mimic some exercise responses.In this study,we explored the effects of the HDAC inhibitor Trichostatin A(TSA)on human skeletal muscle myoblasts(HSMMs).Our findings showed that TSA-induced hyperacetylation enhanced myotube fusion and increased the secretion of extracellular vesicles(EVs)enriched with miR-873-3p.These TSA-EVs promoted osteogenic differentiation in human bone marrow mesenchymal stem cells(hBMSCs)by targeting H2 calponin(CNN2).In vivo,systemic administration of TSA-EVs to osteoporosis mice resulted in significant improvements in bone mass.Moreover,TSA-EVs mimicked the osteogenic benefits of exercise-induced EVs,suggesting that HDAC inhibition can replicate exercise-induced bone health benefits.These results demonstrate the potential of TSA-induced muscle-derived EVs as a therapeutic strategy to enhance bone formation and prevent osteoporosis,particularly for individuals unable to exercise.Given the FDA-approved status of various HDAC inhibitors,this approach holds significant promise for rapid clinical translation in osteoporosis treatment. 展开更多
关键词 organs holds muscle
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