Anticancer effect of the extracts from Polyalthia evecta against human hepatoma cell line(HepG2)

Objective:To investigate the anticancer activity of Polyalthia evecta(P.evecta)(Pierre) Finet & Gagnep against human hepatoma cell line(HepG2).Methods:The anticancer activity was based on(a) the cytotoxicity against human hepatoma cells(HepG2) assessed using a neutral red assay and(b) apoptosis induction determined by evaluation of nuclei morphological changes after DAP1 staining.Preliminary phytochemical analysis of the crude extract was assessed by HPLC analysis.Results:The 50% ethanol-water crude leaf extract of P.evecta(EW-L) showed greater potential anticancer activity with high cytotoxicity[IC_(50)=(62.8±7.3)μg/mL]and higher selectivity in HepG2 cells than normal Vero cells[selective index(SI)=7.9].The SI of EW-L was higher than the positive control,melphalan(SI=1.6) and the apoptotic cells(46.4±2.6)%induced by EW-L was higher than the melphalan(41.6±2.1)%(P<0.05).The HPLC chromatogram of the EW-L revealed the presence of various kinds of polyphenolics and flavonoids in it.Conclusions: P.evecta is a potential plant with anticancer activity.The isolation of pure compounds and determination of the bioactivity of individual compounds will be further performed.

Synergistic anticancer effect of the extracts from Polyalthia evecta caused apoptosis in human hepatoma(HepG2)cells

Objective:To evaluate the anticancer activity of the extract fraction of Polyalthia evecta(P. evecta)(Pierre) Finet & Gagnep and the synergistic anticancer effect of the extracts from P.evecta by using the ATR/FT-IR spectroscopy.Methods:The 50%ethanol-water crude leaf extract of P.evecta(EW-L) was prepared and was further fractionated to isolate various fractions.The anticancer activity was investigated from cytotoxicity against HepG2 using a neutral red assay and apoptosis induction by evaluation of nuclei morphological changes after DAPI staining. Synergistic anticancer effects of the extracts from P.evecta were performed using the ATR/FTIR spectroscopy.Results:The result showed that the EW-L showed higher cytotoxicity and apoptosis induction in HepG2 cells than its fractionated extracts.The hexane extract exhibited higher cytotoxicity and apoptosis induction than the water extracts,but less than the EW-L. The combined water and hexane extracts apparently increased cytotoxicity and apoptosis induction.The%apoptotic cells induced by the extract mixture were increased about 2-fold compared to the single hexane extract Conclusions:The polar extract fraction is necessary for the anticancer activity of the non-polar extract fraction.The ATR/FT-IR spectra illustrates the physical interaction among the constituents in the extract mixture and reveals the presence of polyphenolic constituents in the EW-L,which might play a role for the synergistic anticancer effect.

蝶豆花提取物的抗氧化活性及其对酮康唑诱导大鼠睾丸损伤的保护作用(英文)

研究目的:研究蝶豆花提取物的抗氧化活性及其对酮康唑(KET)诱导雄性大鼠睾丸损伤的保护作用。创新要点:对蝶豆花提取物保护酮康唑引起的雄性动物睾丸损伤进行研究,为减轻抗真菌药物酮康唑的生殖毒性提供理论依据和解决途径。研究方法:采用2,2-二苯基-1-苦肼基自由基(DPPH)分析法和亚铁还原能力实验法(FRAP)来测定蝶豆花提取物的抗氧化活性。在为期28天的动物试验中,前21天为预防期,第21至28天为KET诱导期。整个试验期中,对雄性大鼠进行蝶豆花提取物(0、50、100 mg/kg BW)灌胃饲养;在诱导期,同时腹腔注射KET(100 mg/kg BW)。试验结束后,测定睾丸、附睾和输精管以及精囊的重量、血清睾酮水平、精子浓度、组织结构、睾丸的直径和睾丸酪氨酸磷酸化水平。重要结论:蝶豆花提取物具有清除DPPH自由基能力和较高的还原能力,其在100 mg/kg BW下对雄性大鼠生殖系统无毒。50、100 mg/kg BW蝶豆花提取物能改善KET引起的生殖器官重量下降、血清睾酮水平和精子浓度,降低KET引起的睾丸损伤。与其他组相比,100 mg/kg BW蝶豆花提取物能显著提高睾丸中50-kDa的酪氨酸磷酸化蛋白的表达。由此可见,具有抗氧化活性的蝶豆花提取物不会损伤雄性生殖系统,而且具有保护KET诱导大鼠睾丸损伤的作用。

Phyllanthus emblica叶提取物对慢性应激大鼠睾丸损伤有改善作用（英文）

目的:探讨Phyllanthus emblica L.(PE)叶提取物对慢性应激大鼠睾丸损伤和相关蛋白标志物变化的影响。方法:将成年雄性大鼠分成四组:对照组、慢性应激(CS)组和两组PE提取物治疗组(50或100mg/kg体重(BW))。对各组的精子质量、睾丸和尾附睾的组织学特征、以及血清皮质酮、睾酮和丙二醛(MDA)的水平进行分析比较。并使用免疫印迹分析研究睾丸类固醇合成快速调节蛋白(StAR)和酪氨酸磷酸化蛋白的表达。结论:结果显示,50mg/kgBW的PE能显着增加精子浓度和睾酮水平,同时降低CS大鼠中皮质酮水平、MDA水平、以及精子头异常和顶体反应精子的比例。此外,两种剂量的PE都能缓解CS大鼠的睾丸组织病理学特征。同时我们还发现,50 mg/kg BW的PE可以显著提高StAR蛋白的表达,并改变睾丸中部分酪氨酸磷酸化蛋白的强度。总之,50 mg/kgBW的PE叶提取物可以预防CS大鼠的睾丸损伤。

泰国余甘子水提物中总酚含量及抗氧化活性(英文)

通过对余甘子不同部位(叶、枝和树皮)水提物的总酚含量的测定及体外抗氧化活性的研究,为其体内及临床试验研究奠定了基础。余甘子叶、枝和树皮的水提物皆具有抗氧化活性,其中树皮中的总酚含量最高,抗氧化活性最强。

束缚应激对雄性大鼠睾丸磷酸化蛋白表达的影响（英文）

目的:寻找雄性大鼠应激模型中潜在的睾丸蛋白标记物,探索评估男性生殖能力新的参数。创新点:压力可以影响男性生育能力,但其具体机制尚未明了。睾丸组织中酪氨酸磷酸化蛋白在精子形成过程中扮演着重要的角色。本研究首次利用动物模型说明了压力对睾丸组织中酪氨酸磷酸化蛋白表达的影响,进而探索评估男性生殖能力新的参数。方法:雄性SD大鼠被随机分为对照组和束缚应激的实验组。实验组大鼠每天禁足12小时,持续7天。观察比较两组类固醇激素、精子密度、生殖系统形态学和组织学的差别,同时利用免疫印迹方法比较两组睾丸组织裂解产物中急性类固醇调节蛋白和酪氨酸的表达差别(早期在支持细胞中表达,后期在精子细胞中表达)。结果:实验组大鼠的睾酮水平和精子密度较对照组均显著降低,而精子畸形率和皮质酮显著升高。组织学比较发现实验组大鼠精子团数量减少并且出现输精管萎缩。两组大鼠睾丸组织中酪氨酸磷酸化蛋白的表达具有显著差异,实验组95 k Da的磷酸化蛋白表达量显著降低,但两组急性类固醇调节蛋白的表达没有显著差异。结论:本研究证明束缚应激可使大鼠睾丸组织磷酸化蛋白表达降低,并相应伴随着生殖能力参数的降低。这也许可以从一个方面解释压力对男性不育的影响。