Helicobacter Increase Instability Genome in Mucous Cells of Antrum of Stomach Mucous in People, Who Lives in Radiation Contaminated Areas

The research of degree of incidence of genomic changes (of micronuclei) in mucous cells of gastric superficial-foveolar epithelium in contaminated and not contaminated mucous of stomach was made. Histopathological research of micronuclei and helicobacter was made in gastric biopsy specimen of patients with diagnosis-chronical gastritis (ICD-10K29.3) in group of patients who lived in radiation contaminated areas and in groups of people, who had no factors of radiation influence in anamnesis. People, who lived in the radiation contaminated areas, whose mucous was infected by Helicobacter pylori, had the highest frequency of mucous cells with micronuclei. In the group of patients from radiation contaminated areas with HP-associated gastritis frequency of appearance of mucous cells with micronucleus in the mucous of stomach have five time more, than patients, whose mucous of stomach was not infected (p Helicobacter pylori can success in mutagenic effect of radiation factor.

Epidemic Outbreak of Measles in the Region of Plovdiv, Bulgaria (March-July 2017)

Object: To analyze the clinical, epidemiological and laboratory characteristics of the cases with measles in the Plovdiv region, Bulgaria, in the period March-July 2017. Materials and Methods: The study included 139 hospitalized patients with measles, treated in the Clinic of Infectious Diseases, University Hospital “St. George”—Plovdiv, during the observed period. Diagnosis was verified by ELISA in 133 of the patients. The following methods were used: clinical and epidemiological analysis, laboratory, microbiological and imaging tests. Results: The age distribution of the patients was: infants below 1 year were 23.8%;1 - 3 years—24.5%;4 - 17 years—30.9%;and over 18 years —20.8%. Ethnicity: from Roma origin were 83.5% and Bulgarians—16.5%. Most of the patients (62%) reported contact with a measles patient. Ninety eight of the patients were over 13 months of age and they should have been immunized, as the vaccine in Bulgaria is applied on the 13 month. Of these 98 patients, 49% were immunized, 28% were not, and for 23% there were not data. The most common clinical symptoms were: fever—97.1%, cough—98.6% and runny nose—82.7%. Conjunctivitis was observed in 82%, asthenia in 46%, and diarrhea in 51.1%. The rash appeared average on the 4th day of the onset of complaints. Koplik’s spots were observed in 71.9%, cervical lymphadenopathy in 56.1%, hepatomegaly—in 25.2%, and splenomegaly—in 7.9%;auscultation data for bronchitis/pneumonia—in 33.1%. Laboratory tests: leucopenia in 32.4%, leukocytosis—2.2%, normal results—in the rest 65.4%. ESR was increased in 39.1% and CRP in 76.5% of the patients. X-ray of the lung was performed under indication and was positive in 28/46. The serological test—anti-measles IgM (ELISA) was positive in 95.7% (133/139). We observed the following complications: pneumonia—12.2%, bronchitis—15.1%, laryngitis—4.3%, otitis—1.4%, gastroenteritis—14.4% and stomatitis—6.5%. The outcome was fatal for one patient. Conclusions: Measles still prevails during the age of childhood, but 20.8% of the patients were adults. The analysis of the patient’s immunity showed that the lap of immunization was the main cause for periodical arising of epidemic outbreaks, although the programs for elimination of measles in Europe until 2020. The disease ran with typical course but we observed a higher rate of complications.

Optimal Use of Skin Thiersch Graft

The most popular and most widely used mode for short-term storage of grafts was curing them in a standard fridge at a temperature of＋4 ℃. According to the literature, the usability of the same ranges from 10 clays to 2! days. The aim was to determine the usefulness of such a maximum time preserved grafts. This study comprised 50 patients, of which, take parts of skin with the size of 3 cm × 5 cm. It started from the 11th day after the 5 preparation were divided, one of which is a part applied to a suitable surface for accepting the transplant, the second part of the extended conservation 10 more days （to be transplanted） and the third placed in formalin and sent to histopatological analysis. Then, a series of five preparations were made, in which each subsequent extended period of conservation by one day longer, ending their 20th day. Usability over 50% of grafts preserved in NaCl solutions up to 20 days, and then felt. It is possible that a transplant is used with 30 days of preservation, but this is the exception, not the usual state.

切尔诺贝利事故清理工作者淋巴细胞的尾核

为了研究切尔诺贝利事故清理工作者和其他受照射人员外周血淋巴细胞,选取切尔诺贝利事故清理工作者和其他受照射人员作为试验组,采用常规方法取指尖外周血制备涂片,空气干燥,对氨基苯甲酸混合,Giemsa法染色,光镜下计数每人500个细胞样本,观察记录具有尾状突起细胞核发生率,进行统计学处理。未照射对照组的外周血淋巴细胞尾核发生率在0%～0.6%,平均值0.16。而大多数受照射对象都出现较多具有尾核的淋巴细胞。照射组的淋巴细胞出现尾核最大值是3.2%,平均值是0.50%。切尔诺贝利事故清理工作者的带尾核的淋巴细胞出现的几率显著高于对照组(p<0.01)。外周血淋巴细胞尾核可能是一种有意义的受照射标记物,受照射淋巴细胞尾核的异常出现可能起因于双着丝粒所形成的染色体桥的断裂。对于更加详尽地获取辐射生物效应信息,回顾性研究辐射剂量效应可能具有重要的理论和实践价值。

Prehospital response to respiratory distress by the public ambulance system in a Ukrainian city

BACKGROUND: The capability of the public ambulance system in Ukraine to address urgent medical complaints in a prehospital environment is unknown. Evaluation using reliable sources of patient data is needed to provide insight into current treatments and outcomes. METHODS: We obtained access to de-identifi ed computer records from the emergency medical services(EMS) dispatch center in Poltava, a medium-sized city in central Ukraine. Covering a fi vemonth period, we retrieved data for urgent calls with a patient complaint of respiratory distress. We evaluated ambulance response and treatment times, field diagnoses, and patient disposition, and analyzed factors related to fatal outcomes. RESULTS: Over the f ive-month period of the study, 2,029 urgent calls for respiratory distress were made to the Poltava EMS dispatch center. A physician-led ambulance typically responded within 10 minutes. Seventy-seven percent of patients were treated and released, twenty percent were taken to hospital, and three percent died in the prehospital phase. On univariate analysis, age over 60 and altered mental status at the time of the call were strongly associated with a fatal outcome. CONCLUSION: The EMS dispatch center in a medium-sized city in Ukraine has adequate organizational infrastructure to ensure that a physician-led public ambulance responds rapidly to complaints of respiratory distress. That EMS system was able to manage most patients without requiring hospital admission. However, a prehospital fatality rate of three percent suggests that further research is warranted to determine training, equipment, or procedural needs of the public ambulance system to manage urgent medical conditions.

Di-and tri-methylation of histone H3K36 play distinct roles in DNA double-strand break repair

Histone H3 Lys36(H3K36)methylation and its associated modifiers are crucial for DNA double-strand break(DSB)repair,but the mechanism governing whether and how different H3K36 methylation forms impact repair pathways is unclear.Here,we unveil the distinct roles of H3K36 dimethylation(H3K36me2)and H3K36 trimethylation(H3K36me3)in DSB repair via non-homologous end joining(NHEJ)or homologous recombination(HR).Yeast cells lacking H3K36me2 or H3K36me3 exhibit reduced NHEJ or HR efficiency.y Ku70 and Rfa1 bind H3K36me2-or H3K36me3-modified peptides and chromatin,respectively.Disrupting these interactions impairs y Ku70 and Rfa1 recruitment to damaged H3K36me2-or H3K36me3-rich loci,increasing DNA damage sensitivity and decreasing repair efficiency.Conversely,H3K36me2-enriched intergenic regions and H3K36me3-enriched gene bodies independently recruit y Ku70 or Rfa1 under DSB stress.Importantly,human KU70 and RPA1,the homologs of y Ku70 and Rfa1,exclusively associate with H3K36me2 and H3K36me3 in a conserved manner.These findings provide valuable insights into how H3K36me2 and H3K36me3 regulate distinct DSB repair pathways,highlighting H3K36 methylation as a critical element in the choice of DSB repair pathway.

Effect of Xuefu Zhuyu Capsule (血府逐瘀胶囊) on the Symptoms and Signs and Health-related Quality of Life in the Unstable Angina Patients with Blood-stasis Syndrome after Percutaneous Coronary Intervention: A Randomized Controlled Trial

Objective： Compared with Shengmai Capsule （生脉胶囊, SM）, the study was conducted to evaluate the efficacy and safety of Xuefu Zhuyu Capsule （血府逐瘀胶囊, XFZY） on the symptoms and signs and health-related quality of life （HR-QOL） in the unstable angina （UA） patients with blood-stasis syndrome （BSS） after percutaneous coronary intervention （PCI）. Methods： A randomized, double-blinded, doubledummy, and placebo-controlled trial was applied. Ninety patients, diagnosed as UA and BSS after successful PCI, were enrolled and equally randomized into three groups, XFZY group, SM group, and placebo group, and administered with the corresponding medications respectively for four weeks. The clinical symptoms and signs （CSS）, electrocardiography （ECG）, and BSS scores were recorded and compared among groups during and after the treatment. Short-form 36 （SF-36） and Seattle Angina Questionnaire （SAQ） were applied to assess the HR-QOL in each group before and after the treatment. Safety indexes （blood routine and liver and kidney function tests） were also examined at the beginning and after the treatment. Results： Eighty-six patients completed the whole study. After the treatment, the total effective rates of the XFZY group in ameliorating CSS and ECG were 76.7% and 60.0%, respectively, which were obviously higher than those in SM （CSS： 53.3%; ECG： 36.7%） and the placebo （CSS： 43.3%; ECG： 30.0%） groups. After one week＇s treatment, BSS scores slightly decreased in each group, but no significant differences were found among three groups （P0.05）. After four weeks＇ treatment, BSS scores in the XFZY group decreased to a lower level compared with SM （P0.05） and the placebo （P0.01） groups. After the treatment, the efficacy of XFZY group in improving body pain （BP）, general health （GH）, vitality （VT）, society functioning （SF）, role emotional （RE）, angina stability （AS）, angina frequency （AF）, and treatment satisfaction （TS） were better than those in the placebo group （P0.05, P0.01）. Meanwhile, the dimensions of BP, GH, SF, AS, AF, and TS were better improved than those in the SM group （P0.05）. No obvious adverse reaction was found during and after the treatment except one case in the XFZY group reporting of stomach discomfort. Conclusions： Compared with SM Capsule treatment, a short-term treatment with XFZY Capsule exhibits better efficacy on CSS and BSS scores, and HR-QOL in UA patients with BSS after PCI. However, its long-term efficacy and safety still needs further investigation.

Serial analysis of gene expression in mice with lipopoly-saccharide-induced acute lung injury

Objective: To monitor the systemic gene expression profile in a murine model of lipopolysaccharide-induced acute lung injury. Methods: Acute lung injury was induced by intratracheal injection of lipopolysaccharide in 3 mice. Another 3 normal mice receiving same volume of normal saline were taken as the controls. The comprehensive gene expression profile was monitored by the recently modified long serial analysis of gene expression. Results: A total of 24 670 tags representing 12 168 transcripts in the control mice and 26 378 tags representing 13 397 transcripts in the mice with lung injury were identified respectively. There were 11 transcripts increasing and 7 transcripts decreasing more than 10 folds in the lipopolysaccharide-treated mice. The most overexpressed genes in the mice with lung injury included serum amyloid A3, metallothionein 2, lipocalin 2, cyclin-dependent kinase inhibitor 1A, lactate dehydrogenase 1, melatonin receptor, S100 calcium-binding protein A9, natriuretic peptide precursor, etc. Mitogen activated protein kinase 3, serum albumin, complement component 1 inhibitor, and ATP synthase were underexpressed in the lung injury mice. Conclusions: Serial analysis of gene expression provides a molecular characteristic of acute lung injury.

切尔诺贝利核事故受照射人员生物标本数据库的建立

目的建立一种简易的核事故受照射人员生物标本库,准备用于研究小剂量电离辐射对人体作用。方法将血液标本固定于滤膜上加以封存,血液标本常规涂片染色保存和保存一部分头发。结果收集1162例人体生物标本,其中958例切尔诺贝利核事故清理工作人员,46例其他核事故受照射人员,158例对照组人员,这些生物标本附有完整的信息数据。结论建立的生物标本数据库对于更加详尽获取辐射生物效应信息、回顾性研究辐射对人类机体作用机制,具有重要的理论和实践价值。

Bridging peripheral nerve defect with chitosan-collagen film

Objective: To seek new method for the treatment of peripheral nerve injury. Methods: In rat model with sciatic nerve defect, chitosan collagen film was sutured into conduit to bridge 5 mm , 10 mm nerve defects. Rats that underwent end to end anastomosis were taken as controls. General observation, electrophysiological study, histological study and image analysis were performed at 4, 8, 12 weeks postoperatively. Results: In 5 mm nerve defects, the quality of nerve regeneration was similar to that of the control group. For 10 mm nerve defect, nerve regeneration was inferior to that of the control group. Chitosan collagen film obviously degraded at 12 weeks postoperatively. Conclusions: Chitosan collagen film conduit can be used to bridge peripheral nerve defect.