Nanopore-based devices have provided exciting opportunities to develop affordable label-free DNA sequencing platforms.Over a decade ago,graphene has been proposed as a two-dimensional(2D)nanopore membrane in order to ...Nanopore-based devices have provided exciting opportunities to develop affordable label-free DNA sequencing platforms.Over a decade ago,graphene has been proposed as a two-dimensional(2D)nanopore membrane in order to achieve single-base resolution.However,it was experimentally revealed that clogging of the graphene nanopore can occur due to the hydrophobic nature of graphene,thus hindering the translocation of DNA.To overcome this problem,the exploration of alternative 2D materials has gained considerable interest over the last decade.Here we show that a Ti_(2)C-based MXene nanopore functionalized by hydroxyl groups(–OH)exhibits transverse conductance properties that allow for the distinction between all four naturally occurring DNA bases.We have used a combination of density functional theory and non-equilibrium Green’s function method to sample over multiple orientations of the nucleotides in the nanopore,as generated from molecular dynamics simulations.The conductance variation resulting from sweeping an applied gate voltage demonstrates that the Ti_(2)C-based MXene nanopore possesses high potential to rapidly and reliably sequence DNA.Our findings open the door to further theoretical and experimental explorations of MXene nanopores as a promising 2D material for nanopore-based DNA sensing.展开更多
基金support from the Thailand Research Fund(MRG6280150)S.J.is supported by Suranaree University of Technology(SUT)+3 种基金Thailand Science Research and Innovation(TSRI)National Science,Research and Innovation Fund(NSRF grant No.B05F640051)R.G.A.thanks for financial support from CNPq(Nos.437182/2018-5 and 313076/2020-0)also FAPERJ(Nos.E-26/010.101126/2018 and 26/202.699/2019).R.H.S.ackn。
文摘Nanopore-based devices have provided exciting opportunities to develop affordable label-free DNA sequencing platforms.Over a decade ago,graphene has been proposed as a two-dimensional(2D)nanopore membrane in order to achieve single-base resolution.However,it was experimentally revealed that clogging of the graphene nanopore can occur due to the hydrophobic nature of graphene,thus hindering the translocation of DNA.To overcome this problem,the exploration of alternative 2D materials has gained considerable interest over the last decade.Here we show that a Ti_(2)C-based MXene nanopore functionalized by hydroxyl groups(–OH)exhibits transverse conductance properties that allow for the distinction between all four naturally occurring DNA bases.We have used a combination of density functional theory and non-equilibrium Green’s function method to sample over multiple orientations of the nucleotides in the nanopore,as generated from molecular dynamics simulations.The conductance variation resulting from sweeping an applied gate voltage demonstrates that the Ti_(2)C-based MXene nanopore possesses high potential to rapidly and reliably sequence DNA.Our findings open the door to further theoretical and experimental explorations of MXene nanopores as a promising 2D material for nanopore-based DNA sensing.
