Backgroud: Current understanding of injury and regeneration of islet β-cells in diabetes is mainly based on rodent studies. The tree shrew is now generally accepted as being among the closest living relatives of prim...Backgroud: Current understanding of injury and regeneration of islet β-cells in diabetes is mainly based on rodent studies. The tree shrew is now generally accepted as being among the closest living relatives of primates, and has been widely used in animal experimentation. However, there are few reports on islet cell composition and regeneration of β-cells in tree shrews.Methods: In this study, we examined the changes in islet cell composition and regeneration of β-cells after streptozotocin(STZ) treatment in tree shrews compared with Sprague-Dawley rats. Injury and regeneration of islet β-cells were observed using hematoxylin and eosin(HE) staining and immunohistochemical staining for insulin, glucagon, somatostatin and PDX-1.Results: Our data showed that in rats islet injury was most obvious on day 3 after injection, and islet morphologies were significantly restored by day 21. Regeneration of islet β-cells was very pronounced in rats, and mainly involved regeneration of centro-acinar cells and transformation of extra-islet ductal cells. In tree shrews, the regeneration of islet β-cells was not as significant. On days 3 and 7, only scattered regenerated cells were observed in the remaining islets. Further, no regeneration of centro-acinar cells was observed.Conclusion: The results suggest that the repair mechanism of islet β-cells in tree shrews is similar to that of humans.展开更多
DEAR EDITOR,Osteonecrosis is a common human disease in orthopedics.It is difficult to treat,and half of patients may need artificial joint replacement,resulting in a considerable economic burden and a reduction in qua...DEAR EDITOR,Osteonecrosis is a common human disease in orthopedics.It is difficult to treat,and half of patients may need artificial joint replacement,resulting in a considerable economic burden and a reduction in quality of life.Hormones are one of the major causes of osteonecrosis and high doses of corticosteroids are considered the most dangerous factor.展开更多
基金Laboratory Animal Science of PLA,Grant/Award Number:SYDW[2014]-0009The National Science and Technology Support Program,Grant/Award Number:2011BAI15B01,2014BAI01B01
文摘Backgroud: Current understanding of injury and regeneration of islet β-cells in diabetes is mainly based on rodent studies. The tree shrew is now generally accepted as being among the closest living relatives of primates, and has been widely used in animal experimentation. However, there are few reports on islet cell composition and regeneration of β-cells in tree shrews.Methods: In this study, we examined the changes in islet cell composition and regeneration of β-cells after streptozotocin(STZ) treatment in tree shrews compared with Sprague-Dawley rats. Injury and regeneration of islet β-cells were observed using hematoxylin and eosin(HE) staining and immunohistochemical staining for insulin, glucagon, somatostatin and PDX-1.Results: Our data showed that in rats islet injury was most obvious on day 3 after injection, and islet morphologies were significantly restored by day 21. Regeneration of islet β-cells was very pronounced in rats, and mainly involved regeneration of centro-acinar cells and transformation of extra-islet ductal cells. In tree shrews, the regeneration of islet β-cells was not as significant. On days 3 and 7, only scattered regenerated cells were observed in the remaining islets. Further, no regeneration of centro-acinar cells was observed.Conclusion: The results suggest that the repair mechanism of islet β-cells in tree shrews is similar to that of humans.
基金supported by the National Science and Key Technology Support Program(2014BAI01B01-07)Science and Technology Key Projects of Kunming(2014-04-A-S-01-3072)。
文摘DEAR EDITOR,Osteonecrosis is a common human disease in orthopedics.It is difficult to treat,and half of patients may need artificial joint replacement,resulting in a considerable economic burden and a reduction in quality of life.Hormones are one of the major causes of osteonecrosis and high doses of corticosteroids are considered the most dangerous factor.
