AIM： To study the effect of retrorsine on mouse hepatocyte proliferation. METHODS： Mice and rats were treated respectively with two injections of retrorsine （as retrosine-treated group） or saline （as non-treated ...AIM： To study the effect of retrorsine on mouse hepatocyte proliferation. METHODS： Mice and rats were treated respectively with two injections of retrorsine （as retrosine-treated group） or saline （as non-treated group） at 2 wk intervals. They received a single injection of carbon tetrachloride （CCh） 4 wk later. On d 0, 1, 2, 3, 4, 6, 15 after CCh administration, the animals were killed and their livers were excised. Hematoxylin and eosin （HE） staining and Ki-67 antibody immunohistochemical analysis of liver samples were used to evaluate the pathological changes and hepatocyte proliferation. RESULTS： In rats treated with retrorsine and CCl4, the liver displayed obvious megalocytosis, proliferation of mild bile duct, small hepatocyte-forming nodule, which were not found in liver samples from non-treated group. However, in mice treated with retrorsine combined with CCh, the liver displayed hepatocyte degeneration and necrosis in perivenous areas. There was no obvious difference between retrorsine-treated group and nontreated group. Ki-67 immunohistochemical analysis showed that in rats treated with retrorsine, the positive hepatocytes mainly found in small hepatocyte nodules, were obviously less than those in non-treated group. The mice treated with retrorsine showed that the number of Ki-67 positive hepatocytes was very high and more than that in non-treated group. CONCLUSION： Retrorsine has no effect on mouse hepatocyte proliferation.展开更多
基金Supported by the Major State Basic Research Development Program of China, No.001CB509904National High Technology Research and Development Program of China, 2004AA205010 Shanghai Science & Technology Commission and Shanghai Municipal Education Commission
文摘AIM： To study the effect of retrorsine on mouse hepatocyte proliferation. METHODS： Mice and rats were treated respectively with two injections of retrorsine （as retrosine-treated group） or saline （as non-treated group） at 2 wk intervals. They received a single injection of carbon tetrachloride （CCh） 4 wk later. On d 0, 1, 2, 3, 4, 6, 15 after CCh administration, the animals were killed and their livers were excised. Hematoxylin and eosin （HE） staining and Ki-67 antibody immunohistochemical analysis of liver samples were used to evaluate the pathological changes and hepatocyte proliferation. RESULTS： In rats treated with retrorsine and CCl4, the liver displayed obvious megalocytosis, proliferation of mild bile duct, small hepatocyte-forming nodule, which were not found in liver samples from non-treated group. However, in mice treated with retrorsine combined with CCh, the liver displayed hepatocyte degeneration and necrosis in perivenous areas. There was no obvious difference between retrorsine-treated group and nontreated group. Ki-67 immunohistochemical analysis showed that in rats treated with retrorsine, the positive hepatocytes mainly found in small hepatocyte nodules, were obviously less than those in non-treated group. The mice treated with retrorsine showed that the number of Ki-67 positive hepatocytes was very high and more than that in non-treated group. CONCLUSION： Retrorsine has no effect on mouse hepatocyte proliferation.
