Establishment of method for seroepidermiological detection of human herpes virus 8 infection by using the fusion protein in the prokaryotic expression system as antigen for testing

To establish a sensitive and specific method for seroepidermiological detection of human her- pes virus 8(HHV-8)infection,three potent antigenic proteins encoded by open reading frames(ORFs) K8.1,65 and 73C in genome of HHV-8 were produced as glutathione S-transferase fusion protein in the prokaryotic expression system and was used as antigen for testing.The recombinant fusion protein ex- pressed in the prokaryotic expression vector E.coli BL21 was purified by glutathione Sepharose 4B affin- ity chromatography and was quantitated with SDS-PAGE.All these 3 fusion proteins produced in the pro- karyotic expression system showed good immunogenicity as demonstrated by Western blotting and could be recognized by mixed sera of patients with Kaposi′s sarcoma(KS).The immuno-reactivities of the single or compound fusion protein were determined by means of ELISA and compared with the traditional immu- nofluorescence assay(IFA)to determine their sensitivity and specificity of the test.It was demonstrated that the sensitivity of mixed-antigen ELISA method was significantly higher than that of IFA(81.8% vs 34.4%),while the specificity of the former was demonstrated to be 97.9%.The coincidence of the de- tection rate between these two methods was considerably high,approaching up to 90.0%.These results suggest that the mixed antigen ELISA assay appears to be a sensitive and specific method for sero-epide- miological detection of human herpesvirus 8 infection.

Study on mechanism of Shenqu Zhuyu decoction in improving varicocele rats spermatogenesis based on PINK1/Parkin mediated mitophagy and apoptosis

Objective To observe the effect of the Shenqu Zhuyu Decoction(SZD)on varicocele(VC)rats mitophagy and apoptosis in left testis based on PTENinduced putative kinase 1(PINK1)/E3 ubiquitin protein ligase(Parkin)signaling pathway,and to explore its mechanism.Methods Totally 36 male SD rats were randomly divided into sham operation group,model group and SZD group,12 rats in each group.VC rats model was established by partial ligation of the left renal vein in model group and SZD group.At 48th day after successful molding,the rats in SZD group were treated with SZD(0.7 g/100 g)by gavage,and rats in sham operation group and model group were given the equal volume of normal saline by gavage,twice a day for 60 days.

The Termination and Aftermath of the Lomagundi -Jatuli Carbon Isotope Excursions in the Paleoproterozoic Hutuo Group, North China

The Lomagundi-Jatuli Event（LJE） is one of the largest and earliest positive carbon isotope excursions preserving δ^（13）C_（carb） values between ＋5 and ＋16‰ in Paleoproterozoic carbonates worldwide. However, the duration, amplitude and patterns of these excursions remain poorly constrained. The 2.14-1.83 Ga Hutuo Group in the North China Craton is a 〉10 km thick volcano-sedimentary sequence, including 〉5 km thick well-preserved carbonates that were deposited in supra-tidal to sub-tidal environments. C-O isotopic and elemental analyses of 152 least altered samples of the carbonates revealed a three-stage δ^（13）C evolution. It began with an exclusively positive δ^（13）C_（carb）（＋1.3 to ＋ 3.4‰） stage in the ~2.1 Ga carbonate in the Dashiling and Qingshicun Formations, followed by a transition from positive values to oscillating positive and negative values in ~3 000 m thick carbonates of the Wenshan, Hebiancun, Jianancun, and Daguandong Formations, and end with exclusively negative δ^（13）C_（carb） values preserved in 〉 500 m thick dolostones of the Huaiyincun and Beidaxing Formations. It appears that much of the LJE, particularly those extremely positive δ^（13）C_（carb） signals, was not recorded in the Hutuo carbonates. The exclusively positive δ^（13）C_（carb） values（＋1.3 to ＋ 3.4‰） preserved in the lower formations likely correspond to the end of the LJE, whereas the subsequent two stages reflect the aftermath of the LJE and the onset of Shunga-Francevillian event（SFE）. The present data point to an increased influence of oxygen on the carbon cycle from the Doucun to the Dongye Subgroups and demonstrate that the termination of the LJE in the North China Craton is nearly simultaneous with those in Fennoscandia and South Africa.

基于碳量子点的活细胞成像及长程示踪

以邻苯二胺为前驱体,通过一步水热合成法,制备出粒径为4 nm,分散性良好,荧光性能优异,高生物相容性的碳量子点(o-Cdots)。采用多代数荧光追踪,研究碳量子点对于人胃癌细胞(MGC-803)的长程示踪荧光成像效果。结果表明,碳量子点进入细胞,分布于细胞质和细胞核,展现出稳定的荧光标记强度。在紫外光持续照射30 min后,相较于商用的细胞核染剂(DAPI)碳点无明显光漂白现象。在细胞传代至5代后,在细胞内仍然可以清晰的观察到碳量子点的明亮荧光。这些优越的特性使碳量子点成为一种长程追踪成像的理想型无创荧光探针。

A meta-analysis of diagnostic value of gene detection for spontaneous bacterial peritonitis

Objective To systematically assess the diagnostic value of gene detection for spontaneous bacterial peritonitis(SBP).Methods A literature search was performed in the database of Pub Med,Web of Science,Cochrane Library and China National Knowledge Internet(CNKI).Relevant studies on diagnostic value of gene detection

A follow up study on iodine nutrition and goiter rate of residents in excessive iodine intaking areas after ceasing supplementation of iodized salt in Dezhou City Shandong Province in 2013-2014

Objective To investigate the status of iodine content and goiter rate of the residents in excessive iodine intaking areas in Dezhou of Shandong Province after ceasing supplementation of iodized salt.Methods In ten excessive iodine intaking counties(cities,districts)from 2013to 2014,300 residents were selected from each

The effect of Bcl-2 gene silencing on the sensitivity of cell line A549 to chemotherapeutic drugs

Objective To investigate the effects of miRNA-mediated down-regulation of the Bcl-2gene on the chemotherapeutic sensitivities and mRNA transcriptions of sensitivity associated genes in human lung adenocarcinoma

Diagnostic performance of plasma mi-R499 for acute myocardial infarction

Objective To evaluate the diagnostic performance of plasma miR-499 in acute myocardial infarction(AMI)diagnosis.Methods Diagnostic accuracy test was used.The suspected AMI patients,who with chest pain for more than half an hour and been admitted in the SecondPeople’s Hospital of Wuxi and First People’s Hospital of Chuzhou during October 2010 and July 2011,were consecutively and prospectively enrolled in the present study.Sixty apparently healthy individuals were designed as

Cytokines expression and significance in the peripheral blood from mice with temporal lobe epileptic in the acute

Objective To study the expression changes of IL-1β,FN-α6,IFN-γand TNFR-m18 in peripheral blood within 2 hours in epileptic mice.Methods Semi quantitative real-time PCR was used to test the mRNA expression level of IL-1β,FN-α6,IFN-γand TNFR-m18 in peripheral blood from normal and

A study on the relationship between arsenic exposure and H3K4me3 and H3K79me3 in human peripheral leukocyte histone

Objective To observe the effect of arsenic exposure to drinking water on the level of histone 3 lysine 4 trimethylation(H3K4me3)and histone 3 lysine 79 trimethylation(H3K79me3)in peripheral blood leukocytes of human,and to analyze the relationship between arsenic exposure and H3K4me3,H3K79me3 modification levels.Methods A cluster sampling survey was carried out in typical endemic arsenicosis areas of Shanxi and Jilin provinces.Two hundred eighty-one local residents with a drinking water age of≥10 years were selected as the survey subjects.According to the arsenic content of drinking water,the tested population was divided into control group(water arsenic content≤0.01 mg/L,60 cases),low water arsenic exposure group(>0.01-0.05 mg/L,61 cases),medium water arsenic exposure group(>0.05-0.10 mg/L,50 cases),and 110 cases of high water arsenic exposure group(>0.10 mg/L).Drinking water samples,immediate urine samples and peripheral blood samples were collected from the subjects.Arsenic content in drinking water and urinary arsenic content were determined via the atomic fluorescence method;histone H3K4me3 and H3K79me3 in peripheral blood leukocytes were determined by dot blot hybridization(Dot Blotting).Results There were no statistically significant differences in age(61.50,60.00,59.50,59.50 years old),different gender(male:20,27,17,40 cases,female:40,34,33,70 cases),body mass index(BMI),smoking and drinking status between the control group,low,medium and high water arsenic exposure groups.Water arsenic content in the control group,low,medium and high water arsenic exposure groups(median:0.005,0.024,0.076,0.150 mg/L),urinary arsenic content(0.011,0.018,0.061,0.134 mg/L),and water arsenic cumulative exposure levels(0.342,1.641,5.273,7.716 mg)were compared between the groups,the differences were statistically significant(H=256.041,88.615,218.610,P<0.01).In the control group,low,medium and high water arsenic exposure groups,the modification levels of H3K4me3(0.100,0.059,0.083,0.083)and H3K79me3(0.049,0.036,0.055,0.052)in peripheral blood leukocytes were not significantly different(H=1.488,2.097,P>0.05).The levels of H3K4me3 and H3K79me3 in peripheral blood leukocytes were positively correlated with water arsenic content,urinary arsenic content,water arsenic cumulative exposure levels(r=0.245,0.221;0.299,0.318;0.149,0.149;P<0.01 or<0.05);there was a positive correlation between H3K4me3 and H3K79me3 modification levels(r=0.811,P<0.01).Conclusion There is a positive correlation between arsenic exposure through drinking water and the levels of H3K4me3 and H3K79me3 in the peripheral blood leukocytes of the population,but it is necessary to expand the sample size for further study.