RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.Th...RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.These lineswere characterized using 80 decamer primers byamplification.Eighty primers amplified 392scorable DNA fragments out of which 20% werepolymorphic.The lines CNH 123 and CNH 120have similarity(85%)as obtained by the Neiand Lies coefficient of similarity.The展开更多
Selection of transformed tissues in the antibioticmedium is an important step for developingtransgenic plants.The toxic level of G.arboreum and G.hirsutum cotton cultivars ofIndian origin were tested against kanamycin...Selection of transformed tissues in the antibioticmedium is an important step for developingtransgenic plants.The toxic level of G.arboreum and G.hirsutum cotton cultivars ofIndian origin were tested against kanamycinusing different concentrations.Among these 75～100 ug. ml<sup>-1</sup> level of kanamycin was found展开更多
文摘RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.These lineswere characterized using 80 decamer primers byamplification.Eighty primers amplified 392scorable DNA fragments out of which 20% werepolymorphic.The lines CNH 123 and CNH 120have similarity(85%)as obtained by the Neiand Lies coefficient of similarity.The
文摘Selection of transformed tissues in the antibioticmedium is an important step for developingtransgenic plants.The toxic level of G.arboreum and G.hirsutum cotton cultivars ofIndian origin were tested against kanamycinusing different concentrations.Among these 75～100 ug. ml<sup>-1</sup> level of kanamycin was found
