Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized...Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.展开更多
Nanoparticles-based drug delivery systems have attracted significant attention in biomedical fields because they can deliver loaded cargoes to the target site in a controlled manner.However,tremendous challenges must ...Nanoparticles-based drug delivery systems have attracted significant attention in biomedical fields because they can deliver loaded cargoes to the target site in a controlled manner.However,tremendous challenges must still be overcome to reach the expected targeting and therapeutic efficacy in vivo.These challenges mainly arise because the interaction between nanoparticles and biological systems is complex and dynamic and is influenced by the physicochemical properties of the nanoparticles and the heterogeneity of biological systems.Importantly,once the nanoparticles are injected into the blood,a protein corona will inevitably form on the surface.The protein corona creates a new biological identity which plays a vital role in mediating the bio–nano interaction and determining the ultimate results.Thus,it is essential to understand how the protein corona affects the delivery journey of nanoparticles in vivo and what we can do to exploit the protein corona for better delivery efficiency.In this review,we first summarize the fundamental impact of the protein corona on the delivery journey of nanoparticles.Next,we emphasize the strategies that have been developed for tailoring and exploiting the protein corona to improve the transportation behavior of nanoparticles in vivo.Finally,we highlight what we need to do as a next step towards better understanding and exploitation of the protein corona.We hope these insights into the“Yin and Yang”effect of the protein corona will have profound implications for understanding the role of the protein corona in a wide range of nanoparticles.展开更多
基金This work was funded by an Irish Research Council for Science,Engineering and Technology Postdoctoral Fellowship(M.L.)the Marianne and Marcus Wallenberg Foundation(M.L.)+2 种基金the EU FP6 project NanoInteract(NMP4-CT-2006-033231)and the SFI SRC BioNanoInteract(07 SRC B1155)Centre for Nano-Vaccine,Copenhagen,Denmark,and the Swedish Research Council(VR).
文摘Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.
基金This work was supported by the National Natural Science Foundation of China(Nos.32171386,51773098,31971304,21807023,and 32101126)the Science Fund for Creative Research Groups of Nature Science Foundation of Hebei Province(No.B2021201038)the China Postdoctoral Science Foundation(No.2021M700954).
文摘Nanoparticles-based drug delivery systems have attracted significant attention in biomedical fields because they can deliver loaded cargoes to the target site in a controlled manner.However,tremendous challenges must still be overcome to reach the expected targeting and therapeutic efficacy in vivo.These challenges mainly arise because the interaction between nanoparticles and biological systems is complex and dynamic and is influenced by the physicochemical properties of the nanoparticles and the heterogeneity of biological systems.Importantly,once the nanoparticles are injected into the blood,a protein corona will inevitably form on the surface.The protein corona creates a new biological identity which plays a vital role in mediating the bio–nano interaction and determining the ultimate results.Thus,it is essential to understand how the protein corona affects the delivery journey of nanoparticles in vivo and what we can do to exploit the protein corona for better delivery efficiency.In this review,we first summarize the fundamental impact of the protein corona on the delivery journey of nanoparticles.Next,we emphasize the strategies that have been developed for tailoring and exploiting the protein corona to improve the transportation behavior of nanoparticles in vivo.Finally,we highlight what we need to do as a next step towards better understanding and exploitation of the protein corona.We hope these insights into the“Yin and Yang”effect of the protein corona will have profound implications for understanding the role of the protein corona in a wide range of nanoparticles.
