The role of gut microbiota in modulating the durability of cOVID-19 vaccine immunity is yet to be characterised.In this cohort study,we collected blood and stool samples of 121 BNT162b2 and 40 CoronaVac vaccinees at b...The role of gut microbiota in modulating the durability of cOVID-19 vaccine immunity is yet to be characterised.In this cohort study,we collected blood and stool samples of 121 BNT162b2 and 40 CoronaVac vaccinees at baseline,1 month,and 6 months post vaccination(p.v).Neutralisation antibody,plasma cytokine and chemokines were measured and associated with the gut microbiota and metabolome composition.展开更多
In order to increase children’s daily intake of dietary fiber and protein,the effects of soluble dietary fiber(SDF)supplement including high-amylose corn starch(HACS),inulin,fructo-oligosaccharides(FOS),galactose oli...In order to increase children’s daily intake of dietary fiber and protein,the effects of soluble dietary fiber(SDF)supplement including high-amylose corn starch(HACS),inulin,fructo-oligosaccharides(FOS),galactose oligo-saccharides(GOS),β-glucan(BG),arabinogalactan(AG),arabinoxylan(AX),and xylo-oligosaccharides(XOS)on protein digestion of multigrain noodles and the fecal microbiota of school-age children were evaluated by in vitro digestion and fermentation in this study.The addition of SDF in multigrain noodles retained a high protein content,which is beneficial for children intake.Except HACS and inulin,the addition of other SDFs significantly increased protein hydrolysis(p<0.05)during gastrointestinal digestion,which will enhance the protein digestion for the children.In addition,noodles(after digestion)containing SDF could reduce fermentation pH,increase short-chain fatty acid levels,and regulate microbiota composition during in vitro fermentation for 24 h.Noodles with HACS resulted in the highest short-chain fatty acids production during fermentation.Noodles containing FOS,GOS and HACS significantly increased the abundance of Bifidobacterium,Lactobacillus and Clostridium-sensu_stricto_1(p<0.05)at genus level in fecal microbiota during fermentation.Results indicated that FOS,GOS and HACS are more suitable to be added to multigrain noodles to improve protein and fiber intake for school-age children.展开更多
基金The study was supported by the Health and Medical Research Fund(HMRF)Commissioned Research Grant(COVID193002)(F.K.L.C.)Enhanced start-up research grant of CUHK(H.M.T.)+2 种基金Hui Hoy&Chow Sin Lan Charity Fund Limited(S.C.N.)the National Research Foundation of Korea(NRF)grant funded through the Korea government(NRF-2018M3A9H4055203)(C.K.P.M.)Emergency Key Program of Guangzhou Laboratory(Grant No.EKPG22-30-6)(C.K.P.M.).
文摘The role of gut microbiota in modulating the durability of cOVID-19 vaccine immunity is yet to be characterised.In this cohort study,we collected blood and stool samples of 121 BNT162b2 and 40 CoronaVac vaccinees at baseline,1 month,and 6 months post vaccination(p.v).Neutralisation antibody,plasma cytokine and chemokines were measured and associated with the gut microbiota and metabolome composition.
基金financial support from the National Natural Science Foundation of China(32222065,31960464,31901648)Youth Talent project of scientific and technological innovation(S2019GDQN2825)+3 种基金Jiangxi high level talent cultivation project(20204BCJ24006)Project of State Key Laboratory of food science and technology(SKLF-ZZA-202111)Cultivation of national science and technology award project(20192AEI91004)Central Government Guide Local Special Fund Project for Scientific and Technological Development of Jiangxi Prov-ince(20221ZDD02001,20212ZDD02008)were gratefully acknowledged.
文摘In order to increase children’s daily intake of dietary fiber and protein,the effects of soluble dietary fiber(SDF)supplement including high-amylose corn starch(HACS),inulin,fructo-oligosaccharides(FOS),galactose oligo-saccharides(GOS),β-glucan(BG),arabinogalactan(AG),arabinoxylan(AX),and xylo-oligosaccharides(XOS)on protein digestion of multigrain noodles and the fecal microbiota of school-age children were evaluated by in vitro digestion and fermentation in this study.The addition of SDF in multigrain noodles retained a high protein content,which is beneficial for children intake.Except HACS and inulin,the addition of other SDFs significantly increased protein hydrolysis(p<0.05)during gastrointestinal digestion,which will enhance the protein digestion for the children.In addition,noodles(after digestion)containing SDF could reduce fermentation pH,increase short-chain fatty acid levels,and regulate microbiota composition during in vitro fermentation for 24 h.Noodles with HACS resulted in the highest short-chain fatty acids production during fermentation.Noodles containing FOS,GOS and HACS significantly increased the abundance of Bifidobacterium,Lactobacillus and Clostridium-sensu_stricto_1(p<0.05)at genus level in fecal microbiota during fermentation.Results indicated that FOS,GOS and HACS are more suitable to be added to multigrain noodles to improve protein and fiber intake for school-age children.
