In order to obtain high-efficiency organophosphorus solubilizing bacteria, 21 strains of organophosphate solubilizing bacteria were isolated from the rhizosphere soil of Camellia oleifera plants, and the transparent c...In order to obtain high-efficiency organophosphorus solubilizing bacteria, 21 strains of organophosphate solubilizing bacteria were isolated from the rhizosphere soil of Camellia oleifera plants, and the transparent circle method was used for rescreening. Only 4 strains of bacteria could form transparent circle on organophosphorus medium. The D/d value of 4 strains of bacteria was between 1.62 and 2.71, among which the D/d value of strain Y6 was the highest(2.71). The available phosphorus content of the fermentation supernatant was 8.50~14.79 mg/L, which was 7.88~14.17 mg/L higher than that of CK. The strain Y6 had the highest soluble phosphorus content of 14.79 mg/L in the fermentation supernatant, which was 14.17 mg/L higher than that of CK. According to the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis of strain Y6, it is preliminarily determined that strain Y6 is Pseudomonas. Strain Y6 is beneficial to improve the supply of phosphorus in rhizosphere soil of Camellia oleifera and promote the growth of Camellia oleifera. It has great potential in the development of bio-organic fertilizer.展开更多
基金湖南省科技厅重点研发计划(2017N K 2144)长沙市科技局重大专项(kq1804016)+1 种基金湖南农业科技创新联盟项目(2017LM0305)重金属污染耕地安全高效利用湖南省工程研究中心开放研究基金(TGOP-001)。
文摘In order to obtain high-efficiency organophosphorus solubilizing bacteria, 21 strains of organophosphate solubilizing bacteria were isolated from the rhizosphere soil of Camellia oleifera plants, and the transparent circle method was used for rescreening. Only 4 strains of bacteria could form transparent circle on organophosphorus medium. The D/d value of 4 strains of bacteria was between 1.62 and 2.71, among which the D/d value of strain Y6 was the highest(2.71). The available phosphorus content of the fermentation supernatant was 8.50~14.79 mg/L, which was 7.88~14.17 mg/L higher than that of CK. The strain Y6 had the highest soluble phosphorus content of 14.79 mg/L in the fermentation supernatant, which was 14.17 mg/L higher than that of CK. According to the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis of strain Y6, it is preliminarily determined that strain Y6 is Pseudomonas. Strain Y6 is beneficial to improve the supply of phosphorus in rhizosphere soil of Camellia oleifera and promote the growth of Camellia oleifera. It has great potential in the development of bio-organic fertilizer.
