Compound-specific stable isotope analysis of individual amino acids(CSIA-AA)has been widely used in ecological and biogeochemical studies.It has been proven to be powerful in tracing the diet sources and trophic inter...Compound-specific stable isotope analysis of individual amino acids(CSIA-AA)has been widely used in ecological and biogeochemical studies.It has been proven to be powerful in tracing the diet sources and trophic interactions.However,assessing the N sources of mesopelagic fishes has been inconclusive because the mesopelagic fishes’unique domain(water depth ranged from 0 to 1000 m)and unresolved nitrogen isotopes of various forms.This study proposes a new method for coupling instruments(ion chromatography and PreconIRMS)and chemical method of oxidation-reduction of amino acids,and also combinedδ15N of AAs withδ13C of fatty acids(FAs)to analyze the trophic interactions of mesopelagic fishes in the South China Sea(SCS).AAs were isolated by ion chromatography with high peak resolution and collected by an automated fraction collector.The chemical method then converted the AAs into N2 O with a robust oxidation yields and suitable molar ratio of NH2 OH to.Finally,theδ15N of AAs at 20 nmol were measured with a reasonable precision(<0.6‰).With this method,this study report the first batch high precisionδ15N of AAs andδ13C of FAs of mesopelagic fishes collected from SCS.Diaphus luetkeni,Chauliodus minimus and Bathygadus antrodes showed similarδ13C values of 20:4 n-6(~-28‰),while Argyropelecus affinis and Stomias had similar values(~-32‰).These results reflect that mesopelagic fishes had complex diet sources.An increase of 4‰inδ15N of glutamic acid(Glu)was found between piscivorous and planktivorous fishes,which might suggest a trophic discrimination factor of mesopelagic fishes in the SCS.This study usedδ13C of 20:4 n-6 to reveal the diet sources of mesopelagic fishes andδ15N of Glu to clarify trophic level between piscivorous and planktivorous fishes.Thus,this combinative method could therefore ultimately be applied in a variety of deep-sea ecosystem.展开更多
2008农历戊子年春节，春意遍天下，欢乐满中华。在欢乐、祥和的喜庆气氛之中，在国内外渔具界人士的翘首期待之下，第十八届中国国际钓鱼用品贸易展览会（China Fish 2008），于2008年2月17日在苏州国际博览中心盛大开幕，经过三天的专...2008农历戊子年春节，春意遍天下，欢乐满中华。在欢乐、祥和的喜庆气氛之中，在国内外渔具界人士的翘首期待之下，第十八届中国国际钓鱼用品贸易展览会（China Fish 2008），于2008年2月17日在苏州国际博览中心盛大开幕，经过三天的专业、紧张、有序的举办，已于2月19日圆满闭幕。大部分中国制造商和重要的国际渔具制造商齐聚苏州国际博览中心，与较之往届数量更为可观的国内外贸易商见面、洽谈。本届展会再次成为国际渔具界的盛会，一次收获商业硕果和体验展会快乐的契机。展开更多
基金The National Basic Research Program(973 Program)of China under contract No.2014CB441502the National Natural Science Foundation of China under contract No.41876074the Cross-research Center Project by QNLM under contract No.JCZX202007。
文摘Compound-specific stable isotope analysis of individual amino acids(CSIA-AA)has been widely used in ecological and biogeochemical studies.It has been proven to be powerful in tracing the diet sources and trophic interactions.However,assessing the N sources of mesopelagic fishes has been inconclusive because the mesopelagic fishes’unique domain(water depth ranged from 0 to 1000 m)and unresolved nitrogen isotopes of various forms.This study proposes a new method for coupling instruments(ion chromatography and PreconIRMS)and chemical method of oxidation-reduction of amino acids,and also combinedδ15N of AAs withδ13C of fatty acids(FAs)to analyze the trophic interactions of mesopelagic fishes in the South China Sea(SCS).AAs were isolated by ion chromatography with high peak resolution and collected by an automated fraction collector.The chemical method then converted the AAs into N2 O with a robust oxidation yields and suitable molar ratio of NH2 OH to.Finally,theδ15N of AAs at 20 nmol were measured with a reasonable precision(<0.6‰).With this method,this study report the first batch high precisionδ15N of AAs andδ13C of FAs of mesopelagic fishes collected from SCS.Diaphus luetkeni,Chauliodus minimus and Bathygadus antrodes showed similarδ13C values of 20:4 n-6(~-28‰),while Argyropelecus affinis and Stomias had similar values(~-32‰).These results reflect that mesopelagic fishes had complex diet sources.An increase of 4‰inδ15N of glutamic acid(Glu)was found between piscivorous and planktivorous fishes,which might suggest a trophic discrimination factor of mesopelagic fishes in the SCS.This study usedδ13C of 20:4 n-6 to reveal the diet sources of mesopelagic fishes andδ15N of Glu to clarify trophic level between piscivorous and planktivorous fishes.Thus,this combinative method could therefore ultimately be applied in a variety of deep-sea ecosystem.
文摘2008农历戊子年春节，春意遍天下，欢乐满中华。在欢乐、祥和的喜庆气氛之中，在国内外渔具界人士的翘首期待之下，第十八届中国国际钓鱼用品贸易展览会（China Fish 2008），于2008年2月17日在苏州国际博览中心盛大开幕，经过三天的专业、紧张、有序的举办，已于2月19日圆满闭幕。大部分中国制造商和重要的国际渔具制造商齐聚苏州国际博览中心，与较之往届数量更为可观的国内外贸易商见面、洽谈。本届展会再次成为国际渔具界的盛会，一次收获商业硕果和体验展会快乐的契机。
